IL-34 赋予调节性 T 细胞新的非经典功能,以保护神经炎症期间脑屏障的完整性。

Lien Van Hoecke, Janne Verreycken, Lore Van Acker, Laura Amelinck, Junhua Xie, Jonas Castelein, Elien Van Wonterghem, Griet Van Imschoot, Marlies Burgelman, Sarah Vanherle, Ilse Dewachter, Paulien Baeten, Bieke Broux, Roosmarijn E. Vandenbroucke
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引用次数: 0

摘要

近年来,在寻找脑损伤修复策略的过程中,脑相关调节性 T 细胞(Tregs)越来越受到关注。除了其教科书上的免疫调节功能外,Tregs 还是应对脑损伤和修复受损脑组织的关键角色。在这里,我们首次描述了 Tregs 在维持血脑屏障(BBB)和血-脑脊液(CSF)屏障的密封能力方面的一种新的非经典功能。此外,我们还发现细胞因子 IL-34 是这一新揭示的 Treg 功能的关键决定因素。从机理上讲,IL-34 通过调节 BBB 内皮细胞和脉络丛上皮细胞中紧密连接蛋白 ZO-1 的表达和定位来施加影响,从而加强脑屏障的强度。鉴于脑屏障渗漏的概念已经确立,而且免疫成分参与了阿尔茨海默病(AD)和多发性硬化(MS)等神经系统疾病,我们进一步证明了来自复发性多发性硬化(RR-MS)患者、AD 甚至轻度认知障碍(MCI)患者的 Tregs 中 IL-34 表达的减少。值得注意的是,我们的研究揭示了 IL-34 治疗在模拟这些神经系统疾病的小鼠模型中恢复大脑屏障完整性的潜力。这些突破性发现揭示了Tregs、IL-34和脑屏障完整性之间错综复杂的关系。它们为改善神经系统疾病中脑屏障功能障碍的治疗方法提供了新的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IL-34 empowers regulatory T cells with novel non-canonical function to safeguard brain barrier integrity during neuro-inflammation.
In efforts to find reparative strategies for brain damage, brain-associated regulatory T cells (Tregs) have gained increasing attention in recent years. Beyond their textbook immunoregulatory function, Tregs have emerged as key players in the response to brain trauma and the restoration of damaged brain tissue. Here, we are the first to describe a novel, non-canonical function of Tregs in maintaining the sealing capacity of both the blood-brain barrier (BBB) and the blood-cerebrospinal fluid (CSF) barrier. Moreover, we identified the cytokine IL-34 as a critical determinant in this newly unveiled Treg function. Mechanistically, IL-34 exerts its influence by modulating the expression and localization of the tight junction protein ZO-1 in both BBB endothelial cells and choroid plexus epithelial cells, thereby reinforcing the strength of the brain barriers. Given the well-established notion of leaky brain barriers and the involvement of immunological components in neurological diseases such as Alzheimer's disease (AD) and multiple sclerosis (MS), we further demonstrate diminished IL-34 expression in Tregs derived from patients with relapsing-remitting MS (RR-MS) and patients with AD and even mild cognitive impairment (MCI). Remarkably, our study reveals the potential of IL-34 treatment in reinstating the integrity of brain barriers within murine models mimicking these neurological disorders. These ground-breaking findings shed light on the intricate relationship between Tregs, IL-34, and the integrity of brain barriers. They offer novel avenues for therapeutic approaches to ameliorate brain barrier dysfunction in the context of neurological disorders.
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