OsWRKY9 参与转录调控级联,增强广谱抗病性

IF 2.2 4区 生物学 Q2 PLANT SCIENCES
Giha Song, Sang Ryeol Park, Yujeong Jeong, Jinjeong Lee, Duk-Ju Hwang, Nam-Chon Paek, Seungmin Son
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引用次数: 0

摘要

包括 Fusarium fujikuroi 和 Xanthomonas oryzae pv. oryzae(Xoo)在内的多种病原体给水稻(Oryza sativa)造成了严重的产量损失。由于气候变化迅速,预计这种情况还会恶化。因此,鉴定能赋予这些病原体先天免疫力的新基因对全球粮食安全至关重要。WRKY 转录因子参与各种植物过程,包括先天免疫。在水稻中,有 125 个 OsWRKYs,其中一些功能已被报道。然而,许多 OsWRKYs 在水稻免疫中的作用在很大程度上仍然未知。本研究调查了 OsWRKY9 在广谱抗病中的作用。OsWRKY9 转录本在对 F. fujikuroi 和 Xoo 的反应中增加。OsWRKY9的启动子被OsWRKY65间接激活,而OsWRKY65赋予了OsWRKY9对F. fujikuroi和Xoo的广谱抗性。此外,OsWRKY9-overexpressing转基因植物对这两种病原体的抗性增强,其方式与过表达 OsWRKY65 的转基因植物类似。此外,OsWRKY9 还调节了受 OsWRKY65 调控的各种防御相关基因的表达。这些结果表明,OsWRKY65-OsWRKY9 模块增强了对巴卡奈病和细菌性枯萎病的抗性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

OsWRKY9 is Involved in Transcriptional Regulatory Cascade Enhancing Broad-Spectrum Disease Resistance

OsWRKY9 is Involved in Transcriptional Regulatory Cascade Enhancing Broad-Spectrum Disease Resistance

Diverse pathogens, including Fusarium fujikuroi and Xanthomonas oryzae pv. oryzae (Xoo), cause significant yield losses in rice (Oryza sativa). The situation is expected to worsen due to rapid climate change. Thus, identifying novel genes conferring innate immunity against these pathogens is crucial for global food security. WRKY transcription factors are involved in various plant processes, including innate immunity. In rice, there are 125 OsWRKYs, with some functions reported. However, the roles of many OsWRKYs in rice immunity remain largely unknown. In this study, we investigate the role of OsWRKY9 in broad-spectrum disease resistance. OsWRKY9 transcripts increased in response to F. fujikuroi and Xoo. The promoter of OsWRKY9 was indirectly activated by OsWRKY65, which confers broad-spectrum resistance to F. fujikuroi and Xoo. Moreover, OsWRKY9-overexpressing transgenic plants exhibited enhanced resistance to both pathogens in a manner similar to transgenic plants overexpressing OsWRKY65. Additionally, OsWRKY9 modulated the expression of various defense-related genes regulated by OsWRKY65. These results indicate that the OsWRKY65-OsWRKY9 module enhances resistance to bakanae disease and bacterial blight.

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来源期刊
Journal of Plant Biology
Journal of Plant Biology 生物-植物科学
CiteScore
5.40
自引率
3.40%
发文量
29
审稿时长
1 months
期刊介绍: Journal of Plant Biology, an official publication of the Botanical Society of Korea, is an international journal devoted to basic researches in biochemistry, cellular biology, development, ecology, genetics, molecular biology, physiology, and systematics of plants. The Journal publishes the following categories of paper: Original articles -- For publication in Journal of Plant Biology the manuscript must provide a significant new contribution to our understanding of plants. All areas of plant biology are welcome. No limit on the length, but a concise presentation is encouraged. Reviews -- Invited by the EiC. Brief Communications -- Concise but independent report representing significant contribution to plant science. The Botanical Society of Korea was founded on November 30, 1957 to promote studies, disseminate and exchange information on the field of plant biology. The first issue of The Korean Journal of Botany, the official publication of the society, was published on April 1, 1958. It was published twice a year, but quarterly from 5th volume in 1962. In 1994, it was renamed to Journal of Plant Biology and published in English since 1996. The journal entered its 50th year of publication in 2007.
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