贯叶连翘提取物对蓖麻棘阿米巴滋养体的杀阿米巴作用

IF 1.4 4区 生物学 Q3 BIOLOGY
Gülizar Aydoğdu, Melek çol Ayvaz, Zeynep Kolören, Onur Kolören, Panagiotis Karanis
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引用次数: 0

摘要

本研究调查了贯叶连翘乙醇提取物对卡氏棘阿米巴滋养体的体外效应。研究确定了植物提取物的细胞毒性作用。研究还显示了植物提取物对 DNA 损伤的保护作用。使用体外乙醇提取物(5、10、15、20、25 和 30 毫克/毫升)测定了穿孔草乙醇提取物的阿米巴痢疾活性。将不同浓度的穿孔草乙醇提取物和滋养体合并,以确定植物提取物的阿米巴痢疾活性。细胞毒性采用改良的 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2 H-溴化四氮唑(MTT)测定法进行评估。琼脂糖凝胶电泳法测定了植物提取物对羟基自由基诱导的 DNA 损伤的影响。滋养体在穿心莲乙醇提取物中的生长终止,抑制浓度为 50%(IC50)/24 小时(30 毫克/毫升)。在 5、2.5 和 1.25 毫克/毫升的浓度下,穿孔连翘乙醇提取物在 72 小时内对人类宫颈癌细胞株(HELA)具有更强的抑制作用。穿孔金丝桃提取物对阿卡阿米巴滋养体(棘阿米巴病的病原体)具有阿米巴杀灭潜力。研究结果表明,贯叶连翘提取物可能是开发治疗棘阿米巴病的有效天然药物的候选者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Amoebicidial effect of Hypericum perforatum extract on Acanthamoeba castellani trophozoites

Amoebicidial effect of Hypericum perforatum extract on Acanthamoeba castellani trophozoites

This study investigated the in vitro efficiency of Hypericum perforatum ethanolic extracts against Acanthamoeba castellani trophozoites. Cytotoxic effects of plant extract were determined. The protective action of plant extract against DNA damage was shown in the study, as well. The amoebicidial activity of H. perforatum ethanolic extracts was set on using in vitro ethanolic extraction (in quantities from 5, 10, 15, 20, 25, and 30 mg/ml). Different concentrations of H. perforatum ethanolic extracts and trophozoites were merged to determine the amoebicidial activity of the plant extract. Cytotoxicity was evaluated using a modified 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) assay. The effect of plant extract on hydroxyl radical-induced DNA damage was determined by electrophoresis on agarose gel. The growth of trophozoites terminated in H. perforatum ethanolic extracts with 50% inhibitory concentrations (IC50)/24 h for 30 mg/ml. The same extract solution had more vigorous amoebicidial activity on the trophozoites with IC50/72 h. Hypericum perforatum showed more substantial inhibitory effects on human cervical carcinoma cell lines (HELA) at concentrations of 5, 2.5, and 1.25 mg/mL at 72 h. The ethanolic extract of H. perforatum was more effective against Acanthamoeba and showed DNA protective activity. Hypericum perforatum extract has amoebicidial potential against A. castellani trophozoites, the causative agent of acanthamoebiasis. The results support that H. perforatum extract may be a nominee for developing an effective natural agent for treating acanthamoebiasis.

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来源期刊
Biologia
Biologia 生物-生物学
CiteScore
3.30
自引率
6.70%
发文量
290
审稿时长
6 months
期刊介绍: Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.
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