燃料储存和一天中的时间是造成停留的候鸟血清代谢组变化的原因

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Sean V. Zimin, Anna Zimin, Eyal Shochat, Yariv Brotman, Ofer Ovadia
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引用次数: 0

摘要

候鸟具有出色的表型灵活性,可根据生活史和环境的需要进行生理调整。因此,特别是在自然条件下,辨别迁徙鸟类生理机能的代谢过程具有挑战性。因此,分析快照采样的血清循环代谢物日益成为生理学推断的首选方法,因为这种方法用途广泛,而且很容易适用于候鸟。在本研究中,我们考察了在迁徙过程中起关键作用的脂肪和肌肉综合燃料储存的变化与春季迁徙的欧亚黑冠停歇鸟的血清循环代谢组之间的关系。我们的分析考虑了潜在的时空影响因素,即内盖夫沙漠三个地点的夜间禁食时间和随机的当地条件。我们将重点从预选代谢物的化合物级分析转移到了整体代谢组水平,通过超高效液相色谱-质谱/质谱非靶向代谢组技术对血清循环中的亲脂性和极性分子进行了量化。通过对已注释代谢物的浓度进行广义潜变量线性建模(GLLVM),我们确定了几个候选生物标志物,其中一些在迁徙背景下是新的,特别是与燃料相关的血清神经酰胺的增加可能来自循环中的极低密度脂蛋白(VLDLs)。根据估计的代谢物与燃料和觅食时间的联系以及代谢物之间的残差协变模型,我们证明了燃料与代谢物之间的联系,这种联系总体上与贮存较多的鸟类较高的脂肪动员率和较低的蛋白质动员率以及摄入营养物质随时间积累而降低的燃料利用率相一致,从而为迁徙鸟类的生理学研究引入了一种新方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fuel stores and time of day account for variation in serum metabolomes of passerine migrants stopping over
Migratory birds excel in phenotypic flexibility, adapting physiologically as their life histories and environments require. Discerning the metabolic processes underlying migrants' physiology, an emergent property of multiple continuous and dynamic organism–environment interactions, is therefore challenging, particularly under natural conditions. Accordingly, analyses of snapshot‐sampled serum‐circulating metabolites, versatile and readily applicable for migrating birds, have increasingly become the method of choice for such physiologic inference. However, the atemporal nature of single sampling might obscure the links between observed metabolite concentrations and the processes producing them, necessitating an analytical decoupling of focal processes from their broader biochemical background.In the present study, we examined how variation in combined fat and muscle fuel stores, traits pivotal in migratory context, relates to the serum‐circulating metabolomes of spring‐migrating Eurasian blackcaps stopping‐over. Our analyses accounted for potential spatiotemporal influences in the form of time past night's fasting and random local conditions across three sites within the Negev Desert. We shifted the focus from compound‐level analysis of preselected metabolites towards the level of inclusive metabolome, quantifying serum‐circulating lipophilic and polar molecules via UHPLC–MS/MS untargeted metabolomic technique.Our results indicated a general relationship between fuel stores and the metabolome, comprising 16 326 lipophilic and 6923 polar compounds, among which 918 and 44 were annotated, respectively. By applying generalized latent‐variable linear modeling (GLLVM) upon concentrations of annotated metabolites, we identified several candidate biomarkers, some novel in migratory context, notably the fuel‐associated increase in serum ceramides likely derived from circulating very low‐density lipoproteins (VLDLs). Relying on estimated metabolite links with fuel and foraging time and on modeled residual covariations among metabolites, we demonstrate fuel–metabolite associations generally consistent with higher fat‐ and lower protein mobilization in birds having greater stores and with decreased fuel utilization as ingested nutrients accumulate over time, thus introducing a novel approach for the physiological study of migrating birds.
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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