Karima Ben Mansour, Josef Špak, Petr Komínek, Miloslav Zouhar, Pavel Ryšánek, Adrian J. Gibbs
{"title":"从捷克分离到的菊形目潜伏病毒的基因组序列","authors":"Karima Ben Mansour, Josef Špak, Petr Komínek, Miloslav Zouhar, Pavel Ryšánek, Adrian J. Gibbs","doi":"10.3390/plants13182554","DOIUrl":null,"url":null,"abstract":"The Erysimum latent virus (ELV), a tymovirus, was first isolated from several wild and cultivated brassicas in Germany. Its virions were shown to be serologically distinct from those of the turnip yellow mosaic virus (TYMV), which is also found in wild and cultivated plants in several European countries but also in other parts of the world. TYMV and ELV were among the first plant viruses to have had their genomes sequenced, and when other tymovirus genomes were sequenced, it was found that, in phylogenies, ELV is probably the basal outlier to all other tymoviruses. Here, we report the near-complete genomic sequence of another isolate of ELV from Czechia. This isolate was found in 1990 in Sisymbrium altissimum plants showing mosaic symptoms. It was detected using ELISA tests and electron microscopy. We have now sequenced the full coding sequence of this isolate using contemporary high throughput methods and found that the German and Czech isolates of ELV are closely related and are of the same virus species.","PeriodicalId":20103,"journal":{"name":"Plants","volume":"8 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomic Sequence of a Czech Isolate of Erysimum Latent Virus from Sisymbrium altissimum\",\"authors\":\"Karima Ben Mansour, Josef Špak, Petr Komínek, Miloslav Zouhar, Pavel Ryšánek, Adrian J. Gibbs\",\"doi\":\"10.3390/plants13182554\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The Erysimum latent virus (ELV), a tymovirus, was first isolated from several wild and cultivated brassicas in Germany. Its virions were shown to be serologically distinct from those of the turnip yellow mosaic virus (TYMV), which is also found in wild and cultivated plants in several European countries but also in other parts of the world. TYMV and ELV were among the first plant viruses to have had their genomes sequenced, and when other tymovirus genomes were sequenced, it was found that, in phylogenies, ELV is probably the basal outlier to all other tymoviruses. Here, we report the near-complete genomic sequence of another isolate of ELV from Czechia. This isolate was found in 1990 in Sisymbrium altissimum plants showing mosaic symptoms. It was detected using ELISA tests and electron microscopy. We have now sequenced the full coding sequence of this isolate using contemporary high throughput methods and found that the German and Czech isolates of ELV are closely related and are of the same virus species.\",\"PeriodicalId\":20103,\"journal\":{\"name\":\"Plants\",\"volume\":\"8 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plants\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/plants13182554\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plants","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/plants13182554","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
莴苣潜伏病毒(Erysimum latent virus,ELV)是一种酪病毒,最早是从德国的几种野生和栽培莴苣中分离出来的。其病毒与芜菁黄镶嵌病毒(TYMV)的病毒在血清学上截然不同,后者也存在于欧洲多个国家以及世界其他地区的野生和栽培植物中。TYMV和ELV是最早对其基因组进行测序的植物病毒之一,当对其他斑潜蝇病毒基因组进行测序时,发现在系统发育中,ELV可能是所有其他斑潜蝇病毒的基干。在此,我们报告了来自捷克的另一个ELV分离株的近乎完整的基因组序列。该分离株于 1990 年在出现马赛克症状的茜草植物中发现。它是通过 ELISA 试验和电子显微镜检测到的。现在,我们利用现代高通量方法对该分离株的全编码序列进行了测序,发现德国和捷克的 ELV 分离株关系密切,属于同一病毒种类。
Genomic Sequence of a Czech Isolate of Erysimum Latent Virus from Sisymbrium altissimum
The Erysimum latent virus (ELV), a tymovirus, was first isolated from several wild and cultivated brassicas in Germany. Its virions were shown to be serologically distinct from those of the turnip yellow mosaic virus (TYMV), which is also found in wild and cultivated plants in several European countries but also in other parts of the world. TYMV and ELV were among the first plant viruses to have had their genomes sequenced, and when other tymovirus genomes were sequenced, it was found that, in phylogenies, ELV is probably the basal outlier to all other tymoviruses. Here, we report the near-complete genomic sequence of another isolate of ELV from Czechia. This isolate was found in 1990 in Sisymbrium altissimum plants showing mosaic symptoms. It was detected using ELISA tests and electron microscopy. We have now sequenced the full coding sequence of this isolate using contemporary high throughput methods and found that the German and Czech isolates of ELV are closely related and are of the same virus species.