Luke Chu, Suya Luo, Qionglin Chen, Xiaojiao Chen, Nianjun Xu, Xue Sun
{"title":"通过生理反应和转录组分析揭示 24-表紫苏内酯对拟南芥高温胁迫的有益影响","authors":"Luke Chu, Suya Luo, Qionglin Chen, Xiaojiao Chen, Nianjun Xu, Xue Sun","doi":"10.1007/s00344-024-11454-0","DOIUrl":null,"url":null,"abstract":"<p>The commercial seaweed <i>Gracilariopsis lemaneiformis</i> is mainly used as raw material for agar production and feed for abalone. The heat-resistant strains <i>G</i>. <i>lemaneiformis</i> are extensively cultivated in the northern and southern coasts of China, yet high temperature in the summertime in southern coasts has hindered the growth and limited the cultivated periods of this seaweed<i>.</i> A vast majority of reports have manifested that exogenous phytohormone brassinosteroids (BRs) can improve the plant heat-tolerance. However, little is known about the effect and its underlying mechanism of BRs in algae. In this study, the effect of 24-epibrassinolide (EBR) on the physiological and transcriptional levels was investigated in the high-temperature stressed <i>G. lemaneiformis</i>. Physiological data indicated that EBR application could improve the growth with 1.43-fold on day 5 and non-photochemical quenching parameter, reduce the productions of reactive oxygen species (ROS) and malondialdehyde (MDA), enhance the accumulations of proline, trehalose, and the levels of endogenous 2-methylthio-N6-isopentenyladenine (2MeSiP), 2-methylthio-cis-zeatin riboside (2MeScZR), jasmonic acid (JA), <i>N</i>-[(-)-jasmonoyl]-(l)-phenylalanine (JA-Phe) and salicylic acid 2-<i>O</i>-β-glucoside (SAG) under high-temperature condition. In addition, transcriptomic analysis identified 656 upregulated- and 680 downregulated-genes following the EBR treatment compared to the control group, revealed that EBR activated the metabolic pathways of the glycolysis, tricarboxylic acid (TCA) cycle, pentose phosphate pathway, and synthesis of threonine, methionine, and serine. Taken together, the aforementioned results highlighted the beneficial effect of EBR via alleviating the oxidative stress, promoting the accumulations of osmolytes and stress-related phytohormones, and activating the metabolisms of carbohydrate and amino acid, thereby resulting in the mitigating of growth inhibition by high-temperature stress in <i>G. lemaneiformis</i>.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"60 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Beneficial Effect of 24-Epibrassinolide Against High-Temperature Stress in Gracilariopsis lemaneiformis Revealed by Physiological Response and Transcriptomic Profiling\",\"authors\":\"Luke Chu, Suya Luo, Qionglin Chen, Xiaojiao Chen, Nianjun Xu, Xue Sun\",\"doi\":\"10.1007/s00344-024-11454-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The commercial seaweed <i>Gracilariopsis lemaneiformis</i> is mainly used as raw material for agar production and feed for abalone. The heat-resistant strains <i>G</i>. <i>lemaneiformis</i> are extensively cultivated in the northern and southern coasts of China, yet high temperature in the summertime in southern coasts has hindered the growth and limited the cultivated periods of this seaweed<i>.</i> A vast majority of reports have manifested that exogenous phytohormone brassinosteroids (BRs) can improve the plant heat-tolerance. However, little is known about the effect and its underlying mechanism of BRs in algae. In this study, the effect of 24-epibrassinolide (EBR) on the physiological and transcriptional levels was investigated in the high-temperature stressed <i>G. lemaneiformis</i>. Physiological data indicated that EBR application could improve the growth with 1.43-fold on day 5 and non-photochemical quenching parameter, reduce the productions of reactive oxygen species (ROS) and malondialdehyde (MDA), enhance the accumulations of proline, trehalose, and the levels of endogenous 2-methylthio-N6-isopentenyladenine (2MeSiP), 2-methylthio-cis-zeatin riboside (2MeScZR), jasmonic acid (JA), <i>N</i>-[(-)-jasmonoyl]-(l)-phenylalanine (JA-Phe) and salicylic acid 2-<i>O</i>-β-glucoside (SAG) under high-temperature condition. In addition, transcriptomic analysis identified 656 upregulated- and 680 downregulated-genes following the EBR treatment compared to the control group, revealed that EBR activated the metabolic pathways of the glycolysis, tricarboxylic acid (TCA) cycle, pentose phosphate pathway, and synthesis of threonine, methionine, and serine. Taken together, the aforementioned results highlighted the beneficial effect of EBR via alleviating the oxidative stress, promoting the accumulations of osmolytes and stress-related phytohormones, and activating the metabolisms of carbohydrate and amino acid, thereby resulting in the mitigating of growth inhibition by high-temperature stress in <i>G. lemaneiformis</i>.</p>\",\"PeriodicalId\":16842,\"journal\":{\"name\":\"Journal of Plant Growth Regulation\",\"volume\":\"60 1\",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-08-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Growth Regulation\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00344-024-11454-0\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Growth Regulation","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00344-024-11454-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
The Beneficial Effect of 24-Epibrassinolide Against High-Temperature Stress in Gracilariopsis lemaneiformis Revealed by Physiological Response and Transcriptomic Profiling
The commercial seaweed Gracilariopsis lemaneiformis is mainly used as raw material for agar production and feed for abalone. The heat-resistant strains G. lemaneiformis are extensively cultivated in the northern and southern coasts of China, yet high temperature in the summertime in southern coasts has hindered the growth and limited the cultivated periods of this seaweed. A vast majority of reports have manifested that exogenous phytohormone brassinosteroids (BRs) can improve the plant heat-tolerance. However, little is known about the effect and its underlying mechanism of BRs in algae. In this study, the effect of 24-epibrassinolide (EBR) on the physiological and transcriptional levels was investigated in the high-temperature stressed G. lemaneiformis. Physiological data indicated that EBR application could improve the growth with 1.43-fold on day 5 and non-photochemical quenching parameter, reduce the productions of reactive oxygen species (ROS) and malondialdehyde (MDA), enhance the accumulations of proline, trehalose, and the levels of endogenous 2-methylthio-N6-isopentenyladenine (2MeSiP), 2-methylthio-cis-zeatin riboside (2MeScZR), jasmonic acid (JA), N-[(-)-jasmonoyl]-(l)-phenylalanine (JA-Phe) and salicylic acid 2-O-β-glucoside (SAG) under high-temperature condition. In addition, transcriptomic analysis identified 656 upregulated- and 680 downregulated-genes following the EBR treatment compared to the control group, revealed that EBR activated the metabolic pathways of the glycolysis, tricarboxylic acid (TCA) cycle, pentose phosphate pathway, and synthesis of threonine, methionine, and serine. Taken together, the aforementioned results highlighted the beneficial effect of EBR via alleviating the oxidative stress, promoting the accumulations of osmolytes and stress-related phytohormones, and activating the metabolisms of carbohydrate and amino acid, thereby resulting in the mitigating of growth inhibition by high-temperature stress in G. lemaneiformis.
期刊介绍：
The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches.
The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress.
In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports.
The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
