Kaitlin Gattoni, Eli M. S. Gendron, J. Parr McQueen, Kirsten Powers, Thomas O. Powers, Mary J. Harner, Jessica R. Corman, Dorota L. Porazinska
{"title":"内布拉斯加沙丘地区微生物多样性和集合的性质取决于生物特征和栖息地类型","authors":"Kaitlin Gattoni, Eli M. S. Gendron, J. Parr McQueen, Kirsten Powers, Thomas O. Powers, Mary J. Harner, Jessica R. Corman, Dorota L. Porazinska","doi":"10.1007/s42974-024-00206-5","DOIUrl":null,"url":null,"abstract":"<p>The ecoregion of the Nebraska Sandhills is the largest intact temperate grassland in the world and is impacted by climate change, specifically drought. Despite the well-established role microorganisms play in ecosystem functioning, little is known about microbial community diversity and assembly processes in the Sandhills. To address this gap of knowledge, we examined microbial communities in three habitats (prairie soils, lake sediments, and lake water) across four lake basins ranging in alkalinity (pH 7–11) over two years (October 2020 and 2021). Replicate soil, sediment, and water samples were characterized for bacterial and microbial eukaryotic communities with 16S and 18S rRNA metabarcoding. Analyses for alpha diversity, community composition, and assembly processes were examined with general linear models, β-NTI (nearest taxon indices) analyses, Mantel tests, and modified Hubbell’s neutral models. Overall, diversity and composition varied by habitat type and lake basin regardless of organismal identity or year. Community assembly processes varied by habitat type as well as by organismal identity, but not by year. However, the role of deterministic selection and stochastic dispersal of individual taxa (i.e., OTUs) was interannually variable. Finally, the prairie soil communities were identified as a reservoir for bacterial diversity and the lake sediment communities as a target group for potential monitoring of the effects of climate change in the western Nebraska Sandhills.</p>","PeriodicalId":50994,"journal":{"name":"Community Ecology","volume":"7 1","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The nature of microbial diversity and assembly in the Nebraska Sandhills depends on organismal identity and habitat type\",\"authors\":\"Kaitlin Gattoni, Eli M. S. Gendron, J. Parr McQueen, Kirsten Powers, Thomas O. Powers, Mary J. Harner, Jessica R. Corman, Dorota L. Porazinska\",\"doi\":\"10.1007/s42974-024-00206-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The ecoregion of the Nebraska Sandhills is the largest intact temperate grassland in the world and is impacted by climate change, specifically drought. Despite the well-established role microorganisms play in ecosystem functioning, little is known about microbial community diversity and assembly processes in the Sandhills. To address this gap of knowledge, we examined microbial communities in three habitats (prairie soils, lake sediments, and lake water) across four lake basins ranging in alkalinity (pH 7–11) over two years (October 2020 and 2021). Replicate soil, sediment, and water samples were characterized for bacterial and microbial eukaryotic communities with 16S and 18S rRNA metabarcoding. Analyses for alpha diversity, community composition, and assembly processes were examined with general linear models, β-NTI (nearest taxon indices) analyses, Mantel tests, and modified Hubbell’s neutral models. Overall, diversity and composition varied by habitat type and lake basin regardless of organismal identity or year. Community assembly processes varied by habitat type as well as by organismal identity, but not by year. However, the role of deterministic selection and stochastic dispersal of individual taxa (i.e., OTUs) was interannually variable. Finally, the prairie soil communities were identified as a reservoir for bacterial diversity and the lake sediment communities as a target group for potential monitoring of the effects of climate change in the western Nebraska Sandhills.</p>\",\"PeriodicalId\":50994,\"journal\":{\"name\":\"Community Ecology\",\"volume\":\"7 1\",\"pages\":\"\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-08-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Community Ecology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1007/s42974-024-00206-5\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Community Ecology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s42974-024-00206-5","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ECOLOGY","Score":null,"Total":0}
The nature of microbial diversity and assembly in the Nebraska Sandhills depends on organismal identity and habitat type
The ecoregion of the Nebraska Sandhills is the largest intact temperate grassland in the world and is impacted by climate change, specifically drought. Despite the well-established role microorganisms play in ecosystem functioning, little is known about microbial community diversity and assembly processes in the Sandhills. To address this gap of knowledge, we examined microbial communities in three habitats (prairie soils, lake sediments, and lake water) across four lake basins ranging in alkalinity (pH 7–11) over two years (October 2020 and 2021). Replicate soil, sediment, and water samples were characterized for bacterial and microbial eukaryotic communities with 16S and 18S rRNA metabarcoding. Analyses for alpha diversity, community composition, and assembly processes were examined with general linear models, β-NTI (nearest taxon indices) analyses, Mantel tests, and modified Hubbell’s neutral models. Overall, diversity and composition varied by habitat type and lake basin regardless of organismal identity or year. Community assembly processes varied by habitat type as well as by organismal identity, but not by year. However, the role of deterministic selection and stochastic dispersal of individual taxa (i.e., OTUs) was interannually variable. Finally, the prairie soil communities were identified as a reservoir for bacterial diversity and the lake sediment communities as a target group for potential monitoring of the effects of climate change in the western Nebraska Sandhills.
期刊介绍:
Community Ecology, established by the merger of two ecological periodicals, Coenoses and Abstracta Botanica was launched in an effort to create a common global forum for community ecologists dealing with plant, animal and/or microbial communities from terrestrial, marine or freshwater systems. Main subject areas: (i) community-based ecological theory; (ii) modelling of ecological communities; (iii) community-based ecophysiology; (iv) temporal dynamics, including succession; (v) trophic interactions, including food webs and competition; (vi) spatial pattern analysis, including scaling issues; (vii) community patterns of species richness and diversity; (viii) sampling ecological communities; (ix) data analysis methods.