基于钴铁氧体-氧化石墨烯的热疗和辣椒素在诱导乳腺癌细胞凋亡和抑制端粒酶活性方面的协同效应

IF 1.7 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY
Seyed Ali Lajevardian, Abbas Alibakhshi, Simzar Hosseinzadeh, Fatemeh Mobaraki, Monireh Movahedi, Shadie Hatamie, Maryam Tabarzad, Javad Ranjbari
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引用次数: 0

摘要

辣椒素是红辣椒中一种具有生物活性的植物化学物质。它具有治疗特性,包括抗癌活性。本研究调查了辣椒素潜在的抗端粒酶作用,以及该化合物与钴铁氧体-氧化石墨烯纳米复合材料结合对乳腺癌细胞系的协同抑制作用。为此,研究人员合成并表征了亚铁钴/氧化石墨烯(CoFe2O4/GO)纳米粒子。然后,采用 MTT 法和实时定量 PCR 法分析了不同浓度的辣椒素和 CoFe2O4/GO 纳米粒子及其组合对乳腺癌细胞株(MCF-7 和 MCF-10A)的影响,分别评估了它们对细胞活力的影响以及端粒酶逆转录酶(tert)、Bax 和 Bcl2 基因的表达变化。结果表明,辣椒素和 CoFe2O4/GO 纳米粒子对 MCF-7 细胞株有协同作用,使辣椒素和 CoFe2O4/GO 纳米粒子的 IC50 值分别从 0.1 和 1 毫克/毫升降至 0.05 和 0.5 毫克毫升-1。此外,辣椒素和 CoFe2O4/GO 纳米粒子处理后,端粒酶和 bcl2 基因的表达量减少;相反,bax 基因的表达量则显著增加。因此,辣椒素和 CoFe2O4/GO NPs 可诱导乳腺癌细胞凋亡并抑制其生长。总之,辣椒素和 CoFe2O4/GO NPs 的联合治疗可被视为乳腺癌的有效治疗方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic effect of cobalt ferrite-graphene oxide based hyperthermia and capsaicin to induce apoptosis and inhibit telomerase activity in breast cancer cells
Capsaicin is a bioactive phytochemical of red and chili peppers. It has shown therapeutic properties, including anticancer activities. In this study, the potential anti-telomerase effect of capsaicin, as well as synergic inhibitory effect of this compound in combination with cobalt ferrite-graphene oxide nanocomposites was investigated on breast cancer cell line. For this purpose, cobalt ferrite/graphene oxide (CoFe2O4/GO) nanoparticles were synthesized and characterized. Then, the effect of different concentrations of capsaicin and CoFe2O4/GO nanoparticles, and their combination on the breast adenocarcinoma cell lines (MCF-7 and MCF-10A) were analyzed using MTT assay and quantitative real-time PCR for assessing their effect on the cell viability and the expression changes in telomerase reverse transcriptase (tert), Bax and Bcl2 genes, respectively. The results showed a synergistic effect of capsaicin and CoFe2O4/GO NPs on MCF-7 cell lines that reduced the IC50 value from 0.1 and 1 mg/ml for capsaicin and CoFe2O4/GO nanoparticles, to 0.05 and 0.5 mg ml−1, respectively. Moreover, telomerase and bcl2 genes expression decreased after capsaicin and CoFe2O4/GO NPs treatment; while in contrast, bax gene expression significantly increased. Consequently, capsaicin and CoFe2O4/GO NPs treatment could induce apoptosis and inhibit the growth of breast cancer cells. In conclusion, combinational treatment with capsaicin and CoFe2O4/GO NPs could be considered as an efficient therapeutic regimen for breast cancer.
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来源期刊
Advances in Natural Sciences: Nanoscience and Nanotechnology
Advances in Natural Sciences: Nanoscience and Nanotechnology NANOSCIENCE & NANOTECHNOLOGYMATERIALS SCIE-MATERIALS SCIENCE, MULTIDISCIPLINARY
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