Jing Li, Ning Yu, Can-Can Lv, Long Tie, Jia-Ju Pang, Jin-Wang Zhang, Jun Wang
{"title":"黄檗四倍体的诱导及其对形态学和生物碱含量的影响","authors":"Jing Li, Ning Yu, Can-Can Lv, Long Tie, Jia-Ju Pang, Jin-Wang Zhang, Jun Wang","doi":"10.3390/agronomy14092090","DOIUrl":null,"url":null,"abstract":"Phellodendron amurense Rupr. is a precious medicinal tree species in northeast China. However, P. amurense resources have been severely destroyed due to uncontrolled overharvest and the limited innovation of new germplasms by traditional cross-breeding. In this study, polyploid breeding was introduced to the improvement program of P. amurense. Fifty-four tetraploid plants of P. amurense were first produced by colchicine-induced adventitious bud chromosome doubling in stem segment explants. The induction frequency reached 36.16% (1.0 g L−1 colchicine solution for 48 h treatment) and 50.00% (2.0 g L−1 colchicine solution for 24 h treatment), respectively, showing the high efficiency of the somatic chromosome doubling based on the organogenesis system. Tetraploidization resulted in significant phenotypic variation, such as larger and thicker leaves, thicker stems, and bigger stomata. Ultra-performance liquid chromatography–mass spectrometry (UPLC–MS/MS) analysis identified 59 differentially accumulated alkaloids (DAAs) between the leaf and stem samples of tetraploids, including 32 upregulated and 27 downregulated in stems. For both leaf and stem samples, 18 DAAs were identified between diploids and tetraploids, with 16 DAAs upregulated in tetraploid leaves and 8 upregulated in tetraploid stems, suggesting that polyploidization caused significant alterations in alkaloid contents in leaves and stems of P. amurense. The contents of the main medicinal compounds, such as berberine, jatrorrhizine, phellodendrine, and palmatine, increased significantly in the leaf and/or stem samples after polyploidization. This finding implied that polyploid breeding might be an effective approach for improving P. amurense, beneficial to preserving and exploiting natural resources.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Induction of Tetraploids in Phellodendron amurense Rupr. and Its Effects on Morphology and Alkaloid Content\",\"authors\":\"Jing Li, Ning Yu, Can-Can Lv, Long Tie, Jia-Ju Pang, Jin-Wang Zhang, Jun Wang\",\"doi\":\"10.3390/agronomy14092090\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Phellodendron amurense Rupr. is a precious medicinal tree species in northeast China. However, P. amurense resources have been severely destroyed due to uncontrolled overharvest and the limited innovation of new germplasms by traditional cross-breeding. In this study, polyploid breeding was introduced to the improvement program of P. amurense. Fifty-four tetraploid plants of P. amurense were first produced by colchicine-induced adventitious bud chromosome doubling in stem segment explants. The induction frequency reached 36.16% (1.0 g L−1 colchicine solution for 48 h treatment) and 50.00% (2.0 g L−1 colchicine solution for 24 h treatment), respectively, showing the high efficiency of the somatic chromosome doubling based on the organogenesis system. Tetraploidization resulted in significant phenotypic variation, such as larger and thicker leaves, thicker stems, and bigger stomata. Ultra-performance liquid chromatography–mass spectrometry (UPLC–MS/MS) analysis identified 59 differentially accumulated alkaloids (DAAs) between the leaf and stem samples of tetraploids, including 32 upregulated and 27 downregulated in stems. For both leaf and stem samples, 18 DAAs were identified between diploids and tetraploids, with 16 DAAs upregulated in tetraploid leaves and 8 upregulated in tetraploid stems, suggesting that polyploidization caused significant alterations in alkaloid contents in leaves and stems of P. amurense. The contents of the main medicinal compounds, such as berberine, jatrorrhizine, phellodendrine, and palmatine, increased significantly in the leaf and/or stem samples after polyploidization. This finding implied that polyploid breeding might be an effective approach for improving P. amurense, beneficial to preserving and exploiting natural resources.\",\"PeriodicalId\":7601,\"journal\":{\"name\":\"Agronomy\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agronomy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/agronomy14092090\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agronomy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/agronomy14092090","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
黄柏是中国东北地区的珍贵药用树种。然而,由于无节制的过度采伐和传统杂交育种对新种质创新的限制,黄柏资源遭到严重破坏。在本研究中,多倍体育种被引入到 P. amurense 的改良计划中。通过秋水仙素诱导茎段外植体不定芽染色体加倍,首次培育出 54 株四倍体 P. amurense。其诱导频率分别达到 36.16%(1.0 g L-1 秋水仙素溶液处理 48 小时)和 50.00%(2.0 g L-1 秋水仙素溶液处理 24 小时),显示了基于器官发生系统的体细胞染色体加倍的高效性。四倍体化导致了显著的表型差异,如叶片更大更厚、茎更粗、气孔更大等。超高效液相色谱-质谱(UPLC-MS/MS)分析在四倍体的叶片和茎样本中发现了59种差异积累的生物碱(DAAs),其中茎中上调32种,下调27种。在叶片和茎样本中,二倍体和四倍体之间共鉴定出 18 种 DAAs,其中 16 种 DAAs 在四倍体叶片中上调,8 种 DAAs 在四倍体茎中上调,这表明多倍体化引起了 P. amurense 叶片和茎中生物碱含量的显著变化。小檗碱、药根碱、黄柏碱和巴马汀等主要药用化合物的含量在多倍体化后的叶片和/或茎样本中显著增加。这一发现表明,多倍体育种可能是改良 P. amurense 的有效方法,有利于保护和开发自然资源。
Induction of Tetraploids in Phellodendron amurense Rupr. and Its Effects on Morphology and Alkaloid Content
Phellodendron amurense Rupr. is a precious medicinal tree species in northeast China. However, P. amurense resources have been severely destroyed due to uncontrolled overharvest and the limited innovation of new germplasms by traditional cross-breeding. In this study, polyploid breeding was introduced to the improvement program of P. amurense. Fifty-four tetraploid plants of P. amurense were first produced by colchicine-induced adventitious bud chromosome doubling in stem segment explants. The induction frequency reached 36.16% (1.0 g L−1 colchicine solution for 48 h treatment) and 50.00% (2.0 g L−1 colchicine solution for 24 h treatment), respectively, showing the high efficiency of the somatic chromosome doubling based on the organogenesis system. Tetraploidization resulted in significant phenotypic variation, such as larger and thicker leaves, thicker stems, and bigger stomata. Ultra-performance liquid chromatography–mass spectrometry (UPLC–MS/MS) analysis identified 59 differentially accumulated alkaloids (DAAs) between the leaf and stem samples of tetraploids, including 32 upregulated and 27 downregulated in stems. For both leaf and stem samples, 18 DAAs were identified between diploids and tetraploids, with 16 DAAs upregulated in tetraploid leaves and 8 upregulated in tetraploid stems, suggesting that polyploidization caused significant alterations in alkaloid contents in leaves and stems of P. amurense. The contents of the main medicinal compounds, such as berberine, jatrorrhizine, phellodendrine, and palmatine, increased significantly in the leaf and/or stem samples after polyploidization. This finding implied that polyploid breeding might be an effective approach for improving P. amurense, beneficial to preserving and exploiting natural resources.