使用含有重氮和芳基叠氮弹头的光活性 DNA 在 RNA-DNA 异质双链中进行光交联反应

IF 1.4 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Chemistry Letters Pub Date : 2024-08-24 DOI:10.1093/chemle/upae152

Kazuya Matsuo, Machiko Kitamura, Kentaro Kobata, Daiki Unita, Tomonori Waku, Akio Kobori

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引用次数: 0

摘要

利用具有光活性的寡脱氧核苷酸（ODNs）可以显著增强高时空分辨率的反义效应。这种增强作用源于 RNA-DNA 异质双链体在光照下与互补 RNA 发生光交联，从而提高了稳定性。在本研究中，我们提出了一种利用 2′-氨基-2′-脱氧尿苷制备包含重氮和芳基叠氮弹头的 ODN 的后导入方法。我们研究了这些光活性 ODN 与其互补 RNA 配对时的光交联效率、速率和核碱基偏好。

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Photo-cross-linking reactions in RNA–DNA heteroduplexes using photoreactive DNA incorporated with diazirine and aryl azide warheads

Utilizing photoreactive oligodeoxyribonucleotides (ODNs) presents a promising avenue for significantly enhancing antisense effects with high spatiotemporal resolution. This enhancement arises from the increased stability of RNA–DNA heteroduplexes achieved through photo-cross-linking with complementary RNAs upon light illumination. In this study, we propose a post-introduction approach for preparing ODNs incorporating diazirine and aryl azide warheads, employing 2′-amino-2′-deoxyuridine. We investigate the photo-cross-linking efficiencies, rates, and nucleobase preferences of these photoreactive ODNs when paired with their complementary RNAs.

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来源期刊

Chemistry Letters 化学-化学综合

CiteScore

3.00

自引率

6.20%

发文量

260

审稿时长

1.2 months

期刊介绍： Chemistry Letters covers the following topics: -Organic Chemistry- Physical Chemistry- Inorganic Chemistry- Analytical Chemistry- Materials Chemistry- Polymer Chemistry- Supramolecular Chemistry- Organometallic Chemistry- Coordination Chemistry- Biomolecular Chemistry- Natural Products and Medicinal Chemistry- Electrochemistry