印度半岛三个大象种群的遗传多样性、基因流和遗传结构模式表明了种群的连通性

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Reeta Sharma, Rahul De, Jean-Philippe Puyravaud, Jyotirmayee Parida, A. Sedhupathy, Tamanna Kalam, Abdul Rahim, K. Muthamizh Selvan, N. Arumugam, S. P. Goyal, Priya Davidar
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引用次数: 0

摘要

濒危亚洲象的印度半岛种群分布在西高止山脉和东高止山脉,以及更东北部的中印度东部(ECI)地区。本研究利用从新鲜大象粪便中获得的 DNA,评估了被西高止山脉的帕尔哈特山口(Palghat Gap)分隔开来的南部两个种群(SI1 和 SI2)以及东中印度地区第三个种群的遗传变异、种群结构和基因流动情况。由于这些种群在以前的研究中已被证明在基因上是相关联的,因此对它们的综合基因多样性较高和通过迁徙进行基因流动的假设进行了检验。共对 379 头大象的 10 个微卫星标记进行了基因分型,并对 33 头大象的 D 环区 630 bp 线粒体 DNA(mtDNA)片段进行了测序。确定了四种以前记录的 mtDNA 单倍型:SI1 和 ECI 各有一个单倍型(分别为 BN 和 BL),而 SI2 有两个单倍型(BA 和 BF)。mtDNA 标记表明这些种群之间存在很大的遗传分化,而微卫星数据显示的分化程度适中。这些种群被划分为三个遗传组:SI1、SI2 和 ECI。然而,其中 39% 的个体显示出混合血统,这表明尽管存在自然和人为障碍,基因仍在持续流动。一些第一代男性移民的出现进一步证明了当代基因的流动。性别比例偏向女性,这与现有的人口普查数据一致。这三个种群应作为一个单一的保护单位进行管理,以确保其长期生存能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Patterns of genetic diversity, gene flow and genetic structure of three Peninsular Indian elephant populations indicate population connectivity

Patterns of genetic diversity, gene flow and genetic structure of three Peninsular Indian elephant populations indicate population connectivity

The Peninsular Indian population of the endangered Asian elephant occurs in the Western and Eastern Ghats, and further north-east in the Eastern Central Indian (ECI) range. Using DNA obtained from fresh elephant dung, this study assessed the genetic variation, population structure, and gene flow in the two southern populations, SI1 and SI2, separated by the Palghat Gap in the Western Ghats, and the third population in the ECI range. As these populations have been shown to be genetically associated in previous studies, the hypotheses that their combined genetic diversity would be high and gene flow via migration would be evident, were tested. A total of 379 elephants were genotyped at 10 microsatellite markers, and a 630 bp mitochondrial DNA (mtDNA) fragment from the D-loop region was sequenced from 33 individuals. Four previously documented mtDNA haplotypes were identified: SI1 and ECI each had a single haplotype (BN and BL, respectively), while SI2 had two haplotypes (BA and BF). The mtDNA markers indicated substantial genetic differentiation among the populations, while differentiation using microsatellite data was moderate. The populations were assigned to three genetic groups: SI1, SI2, and the ECI. However, 39% of these individuals showed mixed ancestry, indicating ongoing gene flow despite natural and human-made barriers. Several first-generation male migrants were identified providing further evidence of contemporary gene flow. The sex ratio was female-biased, which is consistent with the existing census data. These three populations should be managed as a single conservation unit to ensure their long term viability.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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