ZP3 噬菌体及其对黄单胞菌(Xanthomonas oryzae pv. oryzae)具有抗菌潜力的内溶素 LysZP 的基因组特征描述

Viruses Pub Date : 2024-09-11 DOI:10.3390/v16091450
Muchen Zhang, Xinyan Xu, Luqiong Lv, Jinyan Luo, Temoor Ahmed, Waleed A. A. Alsakkaf, Hayssam M. Ali, Ji’an Bi, Chengqi Yan, Chunyan Gu, Linfei Shou, Bin Li
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引用次数: 0

摘要

黄单胞菌(Xanthomonas oryzae pv. oryzae,Xoo)是一种重要的细菌病原体,是水稻细菌性叶枯病爆发的罪魁祸首,对全世界的水稻种植构成了重大威胁。有效管理这种病原体对于确保水稻产量和粮食安全至关重要。在这项研究中,我们鉴定并描述了从中国浙江水稻病叶中分离出的新型 Xoo 噬菌体 ZP3,它可能为针对 Xoo 的生物防治策略提供新的见解,并有助于开发防治细菌性叶枯病的创新方法。透射电子显微镜显示,ZP3 的尾巴很短,没有收缩性。基因组测序和生物信息学分析表明,ZP3 的双链 DNA 基因组长度为 44,713 bp,G + C 含量为 52.2%,预测基因为 59 个,与属于 Xipdecavirus 属的其他 OP1 型 Xoo 噬菌体相似。研究人员进一步研究了ZP3的内溶素LysZP的杀菌作用,并认为LysZP的N端跨膜结构域是信号捕获释放序列,可介导LysZP转运至外质。我们的研究有助于人们了解噬菌体与 Xoo 的相互作用,并表明噬菌体 ZP3 及其内溶素 LysZP 可以开发成针对这种植物病原体的生物控制剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genomic Characterization of Phage ZP3 and Its Endolysin LysZP with Antimicrobial Potential against Xanthomonas oryzae pv. oryzae
Xanthomonas oryzae pv. oryzae (Xoo) is a significant bacterial pathogen responsible for outbreaks of bacterial leaf blight in rice, posing a major threat to rice cultivation worldwide. Effective management of this pathogen is crucial for ensuring rice yield and food security. In this study, we identified and characterized a novel Xoo phage, ZP3, isolated from diseased rice leaves in Zhejiang, China, which may offer new insights into biocontrol strategies against Xoo and contribute to the development of innovative approaches to combat bacterial leaf blight. Transmission electron microscopy indicated that ZP3 had a short, non-contractile tail. Genome sequencing and bioinformatic analysis showed that ZP3 had a double-stranded DNA genome with a length of 44,713 bp, a G + C content of 52.2%, and 59 predicted genes, which was similar to other OP1-type Xoo phages belonging to the genus Xipdecavirus. ZP3’s endolysin LysZP was further studied for its bacteriolytic action, and the N-terminal transmembrane domain of LysZP is suggested to be a signal–arrest–release sequence that mediates the translocation of LysZP to the periplasm. Our study contributes to the understanding of phage–Xoo interactions and suggests that phage ZP3 and its endolysin LysZP could be developed into biocontrol agents against this phytopathogen.
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