Nitin Kumar, Min-Lee Yang, Pengfei Sun, Kristina L. Hunker, Jianping Li, Jia Jia, Fangfang Fan, Jinghua Wang, Xianjia Ning, Wei Gao, Ming Xu, Jifeng Zhang, Lin Chang, Yuqing E. Chen, Yong Huo, Yan Zhang, Santhi K. Ganesh
{"title":"CCDC93 基因变异与中枢收缩压升高、动脉松弛功能受损和线粒体功能障碍有关","authors":"Nitin Kumar, Min-Lee Yang, Pengfei Sun, Kristina L. Hunker, Jianping Li, Jia Jia, Fangfang Fan, Jinghua Wang, Xianjia Ning, Wei Gao, Ming Xu, Jifeng Zhang, Lin Chang, Yuqing E. Chen, Yong Huo, Yan Zhang, Santhi K. Ganesh","doi":"10.1371/journal.pgen.1011151","DOIUrl":null,"url":null,"abstract":"Genetic studies of blood pressure (BP) traits to date have been performed on conventional measures by brachial cuff sphygmomanometer for systolic BP (SBP) and diastolic BP, integrating several physiologic occurrences. Genetic associations with central SBP (cSBP) have not been well-studied. Genetic discovery studies of BP have been most often performed in European-ancestry samples. Here, we investigated genetic associations with cSBP in a Chinese population and functionally validated the impact of a novel associated coiled-coil domain containing 93 (<jats:italic>CCDC93</jats:italic>) gene on BP regulation. An exome-wide association study (EWAS) was performed using a mixed linear model of non-invasive cSBP and peripheral BP traits in a Han Chinese population (N = 5,954) from Beijing, China genotyped with a customized Illumina ExomeChip array. We identified four SNP-trait associations with three SNPs, including two novel associations (rs2165468-SBP and rs33975708-cSBP). rs33975708 is a coding variant in the <jats:italic>CCDC93</jats:italic> gene, c.535C>T, p.Arg179Cys (MAF = 0.15%), and was associated with increased cSBP (β = 29.3 mmHg, <jats:italic>P</jats:italic> = 1.23x10<jats:sup>-7</jats:sup>). CRISPR/Cas9 genome editing was used to model the effect of <jats:italic>Ccdc93</jats:italic> loss in mice. Homozygous <jats:italic>Ccdc93</jats:italic> deletion was lethal prior to day 10.5 of embryonic development. <jats:italic>Ccdc93</jats:italic><jats:sup>+/-</jats:sup> heterozygous mice were viable and morphologically normal, with 1.3-fold lower aortic Ccdc93 protein expression (<jats:italic>P</jats:italic> = 0.0041) and elevated SBP as compared to littermate <jats:italic>Ccdc93</jats:italic><jats:sup>+/+</jats:sup> controls (110±8 mmHg vs 125±10 mmHg, <jats:italic>P</jats:italic> = 0.016). Wire myography of <jats:italic>Ccdc93</jats:italic><jats:sup>+/-</jats:sup> aortae showed impaired acetylcholine-induced relaxation and enhanced phenylephrine-induced contraction. RNA-Seq transcriptome analysis of <jats:italic>Ccdc93</jats:italic><jats:sup>+/-</jats:sup> mouse thoracic aortae identified significantly enriched pathways altered in fatty acid metabolism and mitochondrial metabolism. Plasma free fatty acid levels were elevated in <jats:italic>Ccdc93</jats:italic><jats:sup>+/-</jats:sup> mice (96±7mM vs 124±13mM, <jats:italic>P</jats:italic> = 0.0031) and aortic mitochondrial dysfunction was observed through aberrant Parkin and Nix protein expression. Together, our genetic and functional studies support a novel role of <jats:italic>CCDC93</jats:italic> in the regulation of BP through its effects on vascular mitochondrial function and endothelial function.","PeriodicalId":20266,"journal":{"name":"PLoS Genetics","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic variation in CCDC93 is associated with elevated central systolic blood pressure, impaired arterial relaxation, and mitochondrial dysfunction\",\"authors\":\"Nitin Kumar, Min-Lee Yang, Pengfei Sun, Kristina L. Hunker, Jianping Li, Jia Jia, Fangfang Fan, Jinghua Wang, Xianjia Ning, Wei Gao, Ming Xu, Jifeng Zhang, Lin Chang, Yuqing E. Chen, Yong Huo, Yan Zhang, Santhi K. Ganesh\",\"doi\":\"10.1371/journal.pgen.1011151\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Genetic studies of blood pressure (BP) traits to date have been performed on conventional measures by brachial cuff sphygmomanometer for systolic BP (SBP) and diastolic BP, integrating several physiologic occurrences. Genetic associations with central SBP (cSBP) have not been well-studied. Genetic discovery studies of BP have been most often performed in European-ancestry samples. Here, we investigated genetic associations with cSBP in a Chinese population and functionally validated the impact of a novel associated coiled-coil domain containing 93 (<jats:italic>CCDC93</jats:italic>) gene on BP regulation. An exome-wide association study (EWAS) was performed using a mixed linear model of non-invasive cSBP and peripheral BP traits in a Han Chinese population (N = 5,954) from Beijing, China genotyped with a customized Illumina ExomeChip array. We identified four SNP-trait associations with three SNPs, including two novel associations (rs2165468-SBP and rs33975708-cSBP). rs33975708 is a coding variant in the <jats:italic>CCDC93</jats:italic> gene, c.535C>T, p.Arg179Cys (MAF = 0.15%), and was associated with increased cSBP (β = 29.3 mmHg, <jats:italic>P</jats:italic> = 1.23x10<jats:sup>-7</jats:sup>). CRISPR/Cas9 genome editing was used to model the effect of <jats:italic>Ccdc93</jats:italic> loss in mice. Homozygous <jats:italic>Ccdc93</jats:italic> deletion was lethal prior to day 10.5 of embryonic development. <jats:italic>Ccdc93</jats:italic><jats:sup>+/-</jats:sup> heterozygous mice were viable and morphologically normal, with 1.3-fold lower aortic Ccdc93 protein expression (<jats:italic>P</jats:italic> = 0.0041) and elevated SBP as compared to littermate <jats:italic>Ccdc93</jats:italic><jats:sup>+/+</jats:sup> controls (110±8 mmHg vs 125±10 mmHg, <jats:italic>P</jats:italic> = 0.016). Wire myography of <jats:italic>Ccdc93</jats:italic><jats:sup>+/-</jats:sup> aortae showed impaired acetylcholine-induced relaxation and enhanced phenylephrine-induced contraction. RNA-Seq transcriptome analysis of <jats:italic>Ccdc93</jats:italic><jats:sup>+/-</jats:sup> mouse thoracic aortae identified significantly enriched pathways altered in fatty acid metabolism and mitochondrial metabolism. Plasma free fatty acid levels were elevated in <jats:italic>Ccdc93</jats:italic><jats:sup>+/-</jats:sup> mice (96±7mM vs 124±13mM, <jats:italic>P</jats:italic> = 0.0031) and aortic mitochondrial dysfunction was observed through aberrant Parkin and Nix protein expression. Together, our genetic and functional studies support a novel role of <jats:italic>CCDC93</jats:italic> in the regulation of BP through its effects on vascular mitochondrial function and endothelial function.\",\"PeriodicalId\":20266,\"journal\":{\"name\":\"PLoS Genetics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PLoS Genetics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1371/journal.pgen.1011151\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PLoS Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1371/journal.pgen.1011151","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Genetic variation in CCDC93 is associated with elevated central systolic blood pressure, impaired arterial relaxation, and mitochondrial dysfunction
Genetic studies of blood pressure (BP) traits to date have been performed on conventional measures by brachial cuff sphygmomanometer for systolic BP (SBP) and diastolic BP, integrating several physiologic occurrences. Genetic associations with central SBP (cSBP) have not been well-studied. Genetic discovery studies of BP have been most often performed in European-ancestry samples. Here, we investigated genetic associations with cSBP in a Chinese population and functionally validated the impact of a novel associated coiled-coil domain containing 93 (CCDC93) gene on BP regulation. An exome-wide association study (EWAS) was performed using a mixed linear model of non-invasive cSBP and peripheral BP traits in a Han Chinese population (N = 5,954) from Beijing, China genotyped with a customized Illumina ExomeChip array. We identified four SNP-trait associations with three SNPs, including two novel associations (rs2165468-SBP and rs33975708-cSBP). rs33975708 is a coding variant in the CCDC93 gene, c.535C>T, p.Arg179Cys (MAF = 0.15%), and was associated with increased cSBP (β = 29.3 mmHg, P = 1.23x10-7). CRISPR/Cas9 genome editing was used to model the effect of Ccdc93 loss in mice. Homozygous Ccdc93 deletion was lethal prior to day 10.5 of embryonic development. Ccdc93+/- heterozygous mice were viable and morphologically normal, with 1.3-fold lower aortic Ccdc93 protein expression (P = 0.0041) and elevated SBP as compared to littermate Ccdc93+/+ controls (110±8 mmHg vs 125±10 mmHg, P = 0.016). Wire myography of Ccdc93+/- aortae showed impaired acetylcholine-induced relaxation and enhanced phenylephrine-induced contraction. RNA-Seq transcriptome analysis of Ccdc93+/- mouse thoracic aortae identified significantly enriched pathways altered in fatty acid metabolism and mitochondrial metabolism. Plasma free fatty acid levels were elevated in Ccdc93+/- mice (96±7mM vs 124±13mM, P = 0.0031) and aortic mitochondrial dysfunction was observed through aberrant Parkin and Nix protein expression. Together, our genetic and functional studies support a novel role of CCDC93 in the regulation of BP through its effects on vascular mitochondrial function and endothelial function.
期刊介绍:
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