CCDC93 基因变异与中枢收缩压升高、动脉松弛功能受损和线粒体功能障碍有关

IF 4.5 2区 生物学 Q1 Agricultural and Biological Sciences
Nitin Kumar, Min-Lee Yang, Pengfei Sun, Kristina L. Hunker, Jianping Li, Jia Jia, Fangfang Fan, Jinghua Wang, Xianjia Ning, Wei Gao, Ming Xu, Jifeng Zhang, Lin Chang, Yuqing E. Chen, Yong Huo, Yan Zhang, Santhi K. Ganesh
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引用次数: 0

摘要

迄今为止,对血压(BP)特征的遗传研究都是通过肱动脉袖带血压计对收缩压(SBP)和舒张压进行传统测量,并综合了几种生理现象。与中心收缩压(cSBP)相关的遗传学研究还不多。有关血压的遗传发现研究多在欧洲裔样本中进行。在此,我们在中国人群中调查了 cSBP 的遗传关联,并从功能上验证了一个新的相关盘卷结构域含 93(CCDC93)基因对血压调节的影响。在中国北京的一个汉族人群(N = 5954)中,使用定制的 Illumina ExomeChip 阵列对无创 cSBP 和外周血压性状进行了混合线性模型分析,并开展了一项外显子全关联研究(EWAS)。rs33975708是CCDC93基因的编码变异,c.535C>T,p.Arg179Cys(MAF = 0.15%),与cSBP升高有关(β = 29.3 mmHg,P = 1.23x10-7)。CRISPR/Cas9基因组编辑被用来模拟小鼠Ccdc93缺失的影响。同基因 Ccdc93 缺失在胚胎发育第 10.5 天之前是致死的。与同窝 Ccdc93+/+ 对照组相比,Ccdc93+/- 杂合子小鼠存活率高且形态正常,主动脉 Ccdc93 蛋白表达量降低 1.3 倍(P = 0.0041),SBP 升高(110±8 mmHg vs 125±10 mmHg,P = 0.016)。Ccdc93+/- 主动脉的线性肌电图显示,乙酰胆碱诱导的松弛功能受损,而苯肾上腺素诱导的收缩功能增强。对Ccdc93+/-小鼠胸主动脉进行的RNA-Seq转录组分析发现,脂肪酸代谢和线粒体代谢的通路发生了显著的富集。Ccdc93+/-小鼠血浆游离脂肪酸水平升高(96±7mM vs 124±13mM,P = 0.0031),并且通过Parkin和Nix蛋白的异常表达观察到主动脉线粒体功能障碍。总之,我们的遗传和功能研究支持 CCDC93 通过影响血管线粒体功能和内皮功能在血压调节中发挥新的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic variation in CCDC93 is associated with elevated central systolic blood pressure, impaired arterial relaxation, and mitochondrial dysfunction
Genetic studies of blood pressure (BP) traits to date have been performed on conventional measures by brachial cuff sphygmomanometer for systolic BP (SBP) and diastolic BP, integrating several physiologic occurrences. Genetic associations with central SBP (cSBP) have not been well-studied. Genetic discovery studies of BP have been most often performed in European-ancestry samples. Here, we investigated genetic associations with cSBP in a Chinese population and functionally validated the impact of a novel associated coiled-coil domain containing 93 (CCDC93) gene on BP regulation. An exome-wide association study (EWAS) was performed using a mixed linear model of non-invasive cSBP and peripheral BP traits in a Han Chinese population (N = 5,954) from Beijing, China genotyped with a customized Illumina ExomeChip array. We identified four SNP-trait associations with three SNPs, including two novel associations (rs2165468-SBP and rs33975708-cSBP). rs33975708 is a coding variant in the CCDC93 gene, c.535C>T, p.Arg179Cys (MAF = 0.15%), and was associated with increased cSBP (β = 29.3 mmHg, P = 1.23x10-7). CRISPR/Cas9 genome editing was used to model the effect of Ccdc93 loss in mice. Homozygous Ccdc93 deletion was lethal prior to day 10.5 of embryonic development. Ccdc93+/- heterozygous mice were viable and morphologically normal, with 1.3-fold lower aortic Ccdc93 protein expression (P = 0.0041) and elevated SBP as compared to littermate Ccdc93+/+ controls (110±8 mmHg vs 125±10 mmHg, P = 0.016). Wire myography of Ccdc93+/- aortae showed impaired acetylcholine-induced relaxation and enhanced phenylephrine-induced contraction. RNA-Seq transcriptome analysis of Ccdc93+/- mouse thoracic aortae identified significantly enriched pathways altered in fatty acid metabolism and mitochondrial metabolism. Plasma free fatty acid levels were elevated in Ccdc93+/- mice (96±7mM vs 124±13mM, P = 0.0031) and aortic mitochondrial dysfunction was observed through aberrant Parkin and Nix protein expression. Together, our genetic and functional studies support a novel role of CCDC93 in the regulation of BP through its effects on vascular mitochondrial function and endothelial function.
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来源期刊
PLoS Genetics
PLoS Genetics 生物-遗传学
CiteScore
8.10
自引率
2.20%
发文量
438
审稿时长
1 months
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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