大马士革蔷薇精油：抗酪氨酸酶活性和植物化学成分

IF 4.4 2区医学 Q1 PHARMACOLOGY & PHARMACY

Frontiers in Pharmacology Pub Date : 2024-09-11 DOI:10.3389/fphar.2024.1451452

Qiuyan Wu, Wanting Fang, Hao Liu, Zhong Liu, Xuetao Xu

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引用次数: 0

摘要

酪氨酸酶是黑色素合成的关键酶，其天然抑制剂正受到越来越多的关注。大马士革蔷薇精油（RDEO）作为重要的功能性代谢产物，因其生物活性而广为人知。但其对酪氨酸酶的抑制活性尚未得到详细研究。因此，本文全面研究了 RDEO 对酪氨酸酶的抑制作用，并对其进行了植物化学成分分析。活性筛选结果表明，RDEO具有有效的抗酪氨酸酶活性，是一种可逆的混合型抑制剂。CD 检测结果表明，RDEO 可影响酪氨酸酶的构象，从而降低其活性。在 B16F10 细胞中，RDEO（25-100 μg/mL）可抑制细胞内酪氨酸酶的活性，降低黑色素含量。最后，通过气相色谱-质谱分析发现，香茅醇（21.22%）、香叶醇（14.1%）、二十烷（11.03%）、二十烷（6.65%）和 1-壬二烯（5.16%）是 RDEO 的主要植物化学成分。这项研究为大马士革蔷薇精油作为一种潜在的天然酪氨酸酶抑制剂及其在化妆品和医药中的应用提供了数据支持。

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Rosa × damascena Herrm. essential oil: anti-tyrosinase activity and phytochemical composition

Tyrosinase is a key enzyme in melanin synthesis, and its natural inhibitors are receiving increasing attention. Rosa × damascena Herrm. essential oil (RDEO), as important functional metabolites, was widely known due to its biological activities. But its tyrosinase inhibitory activity has not been detailed investigated. Therefore, in this paper, RDEO was comprehensively investigated the tyrosinase inhibitory, followed by the phytochemical composition analysis. Activity screening results showed that RDEO exhibited effective anti-tyrosinase activity and was a reversible and mixed-type inhibitor. CD assay results revealed that RDEO could affect the conformation of tyrosinase to reduce the activity. In B16F10 cells, RDEO (25–100 μg/mL) could inhibit intracellular tyrosinase activity and decrease melanin content. Finally, GC-MS analysis of RDEO found that citronellol (21.22%), geraniol (14.1%), eicosane (11.03%), heneicosane (6.65%) and 1-nonadecene (5.16%) were its main phytochemical compositions. This study provided data support for Rosa × damascena Herrm. essential oil as one potential natural tyrosinase inhibitor and its applications in cosmetics and medicine.

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来源期刊

Frontiers in Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

7.80

自引率

8.90%

发文量

5163

审稿时长

14 weeks

期刊介绍： Frontiers in Pharmacology is a leading journal in its field, publishing rigorously peer-reviewed research across disciplines, including basic and clinical pharmacology, medicinal chemistry, pharmacy and toxicology. Field Chief Editor Heike Wulff at UC Davis is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.