利用形态特征和分子标记对水稻(Oryza sativa L.)基因型的遗传变异、种群结构和聚类分析

IF 1.6 4区 农林科学 Q2 AGRONOMY
Mst Fatamatuzzohora, Md. Sayeedul Islam, Muhammad Fazle Rabbee, Md. Shahadat Hossain, Sang Gu Kang, Mohammad Nurul Matin
{"title":"利用形态特征和分子标记对水稻(Oryza sativa L.)基因型的遗传变异、种群结构和聚类分析","authors":"Mst Fatamatuzzohora, Md. Sayeedul Islam, Muhammad Fazle Rabbee, Md. Shahadat Hossain, Sang Gu Kang, Mohammad Nurul Matin","doi":"10.1007/s42976-024-00569-5","DOIUrl":null,"url":null,"abstract":"<p>Indigenous landraces are heterogeneous and exhibit greater gene diversity, and are therefore an excellent source of variation and material for crop improvement. However, they are very prone to genetic erosion, therefore exploration of genetic variability in landraces is essential step for crop breeding. Discovery of the genetics of a character in rice, that cope adverse condition, is important for maintaining world-food supply. In this study, fifty rice genotypes were assessed for the phenotypic and genetic diversity and their relatedness using morpho-physiological traits of the genotypes. Further clustering and population structure analysis were performed using phenotypic and simple sequence repeat (SSR) markers data through unweighted pair group method with arithmetic mean (UPGMA) and STRUCTURE analysis. Significant variation among yield components were identified. Phenotypic and genotypic coefficients of variability were higher for filled grains, whereas heritability was highest for plant height. Panicle number and 100-grain weight displayed significant relationship with yield. Phylogenetic clustering of the agromorphological traits divided the genotypes into eight clusters, whereas SSR markers yielded ten clusters. SSR markers produced a total of 138 alleles varying from two to nine with 77.53% polymorphic allele having 3.69 alleles per locus (average). The polymorphic information content indices and observed number of alleles varied among markers with an average of 0.649, exhibiting RM336, RM316, and RM287 have the greatest potentiality in exploring genetic diversity among genotypes. Among the genotypes, Dolkochu, Jolduba, BRRI Dhan-32, and Mokbul displayed significant variability, suggesting their suitability as potential parents in hybridization programs. Population structure study demonstrated that the genotypes were structurally diverse that grouped the accessions into two subpopulations (<i>K</i> = 2). Therefore, this research aimed to identify potential genotypes and suitable traits of rice germplasms for breeding programs.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic variation, population structure, and cluster analysis of rice (Oryza sativa L.) genotypes using morphological characteristics and molecular markers\",\"authors\":\"Mst Fatamatuzzohora, Md. Sayeedul Islam, Muhammad Fazle Rabbee, Md. Shahadat Hossain, Sang Gu Kang, Mohammad Nurul Matin\",\"doi\":\"10.1007/s42976-024-00569-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Indigenous landraces are heterogeneous and exhibit greater gene diversity, and are therefore an excellent source of variation and material for crop improvement. However, they are very prone to genetic erosion, therefore exploration of genetic variability in landraces is essential step for crop breeding. Discovery of the genetics of a character in rice, that cope adverse condition, is important for maintaining world-food supply. In this study, fifty rice genotypes were assessed for the phenotypic and genetic diversity and their relatedness using morpho-physiological traits of the genotypes. Further clustering and population structure analysis were performed using phenotypic and simple sequence repeat (SSR) markers data through unweighted pair group method with arithmetic mean (UPGMA) and STRUCTURE analysis. Significant variation among yield components were identified. Phenotypic and genotypic coefficients of variability were higher for filled grains, whereas heritability was highest for plant height. Panicle number and 100-grain weight displayed significant relationship with yield. Phylogenetic clustering of the agromorphological traits divided the genotypes into eight clusters, whereas SSR markers yielded ten clusters. SSR markers produced a total of 138 alleles varying from two to nine with 77.53% polymorphic allele having 3.69 alleles per locus (average). The polymorphic information content indices and observed number of alleles varied among markers with an average of 0.649, exhibiting RM336, RM316, and RM287 have the greatest potentiality in exploring genetic diversity among genotypes. Among the genotypes, Dolkochu, Jolduba, BRRI Dhan-32, and Mokbul displayed significant variability, suggesting their suitability as potential parents in hybridization programs. Population structure study demonstrated that the genotypes were structurally diverse that grouped the accessions into two subpopulations (<i>K</i> = 2). Therefore, this research aimed to identify potential genotypes and suitable traits of rice germplasms for breeding programs.</p>\",\"PeriodicalId\":9841,\"journal\":{\"name\":\"Cereal Research Communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-08-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cereal Research Communications\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s42976-024-00569-5\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cereal Research Communications","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s42976-024-00569-5","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

摘要

本土陆生品种具有异质性,基因多样性较强,因此是作物改良的绝佳变异和材料来源。然而,它们非常容易受到基因侵蚀,因此探索陆稻品种的基因变异性是作物育种的重要步骤。发现水稻应对不利条件的遗传特性对维持世界粮食供应非常重要。在这项研究中,利用基因型的形态-生理特征评估了 50 个水稻基因型的表型和遗传多样性及其亲缘关系。通过算术平均非加权配对组法(UPGMA)和 STRUCTURE 分析法,利用表型和简单序列重复(SSR)标记数据进行了进一步的聚类和种群结构分析。结果表明,各产量成分之间存在显著差异。饱满谷粒的表型变异系数和基因型变异系数较高,而株高的遗传率最高。圆锥花序数和 100 粒重与产量有显著关系。农业形态性状的系统发育聚类将基因型分为 8 个聚类,而 SSR 标记产生了 10 个聚类。SSR 标记共产生 138 个等位基因,从 2 个到 9 个不等,多态等位基因占 77.53%,平均每个位点有 3.69 个等位基因。不同标记的多态信息含量指数和观察到的等位基因数各不相同,平均为 0.649,表明 RM336、RM316 和 RM287 在探索基因型遗传多样性方面具有最大的潜力。在这些基因型中,Dolkochu、Jolduba、BRRI Dhan-32 和 Mokbul 表现出显著的变异性,表明它们适合作为杂交计划中的潜在亲本。种群结构研究表明,基因型在结构上具有多样性,可将加入的品种分为两个亚群(K = 2)。因此,本研究旨在为育种计划鉴定水稻种质的潜在基因型和合适性状。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genetic variation, population structure, and cluster analysis of rice (Oryza sativa L.) genotypes using morphological characteristics and molecular markers

Genetic variation, population structure, and cluster analysis of rice (Oryza sativa L.) genotypes using morphological characteristics and molecular markers

Indigenous landraces are heterogeneous and exhibit greater gene diversity, and are therefore an excellent source of variation and material for crop improvement. However, they are very prone to genetic erosion, therefore exploration of genetic variability in landraces is essential step for crop breeding. Discovery of the genetics of a character in rice, that cope adverse condition, is important for maintaining world-food supply. In this study, fifty rice genotypes were assessed for the phenotypic and genetic diversity and their relatedness using morpho-physiological traits of the genotypes. Further clustering and population structure analysis were performed using phenotypic and simple sequence repeat (SSR) markers data through unweighted pair group method with arithmetic mean (UPGMA) and STRUCTURE analysis. Significant variation among yield components were identified. Phenotypic and genotypic coefficients of variability were higher for filled grains, whereas heritability was highest for plant height. Panicle number and 100-grain weight displayed significant relationship with yield. Phylogenetic clustering of the agromorphological traits divided the genotypes into eight clusters, whereas SSR markers yielded ten clusters. SSR markers produced a total of 138 alleles varying from two to nine with 77.53% polymorphic allele having 3.69 alleles per locus (average). The polymorphic information content indices and observed number of alleles varied among markers with an average of 0.649, exhibiting RM336, RM316, and RM287 have the greatest potentiality in exploring genetic diversity among genotypes. Among the genotypes, Dolkochu, Jolduba, BRRI Dhan-32, and Mokbul displayed significant variability, suggesting their suitability as potential parents in hybridization programs. Population structure study demonstrated that the genotypes were structurally diverse that grouped the accessions into two subpopulations (K = 2). Therefore, this research aimed to identify potential genotypes and suitable traits of rice germplasms for breeding programs.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.40
自引率
6.20%
发文量
92
审稿时长
6-12 weeks
期刊介绍: This journal publishes original papers presenting new scientific results on breeding, genetics, physiology, pathology and production of primarily wheat, rye, barley, oats and maize.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信