Amr Elkelish, Ahmad M. Alqudah, Dalia Z. Alomari, Badriah Saleh Alammari, Moodi Saham Alsubeie, Seham M. Hamed, Samar G. Thabet
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引用次数: 0
摘要
小麦生物强化是解决全球微量营养素缺乏问题的一条大有可为的途径。因此,了解支撑所有矿物质的理想等位基因的遗传基础对于小麦生物强化非常重要。我们对 111 个小麦基因型的核心集合进行了两年的评估,以测量包括镁(Mg)、钙(Ca)、磷(P)和钾(K)在内的宏量营养素含量。我们的研究表明,在这两年中,所有研究的常量营养元素都存在明显的自然表型变异,且遗传率很高。通过全基因组关联扫描(GWAS),发现了 474 个重要的 SNP 标记,这些标记与这两年所有评估的常量营养素都有关联。通过 GWAS 分析,发现几个可靠的基因组区域与不同的潜在候选基因在生物强化小麦中发挥着重要作用。例如,基因 TraesCS1A02G261200 位于染色体 1A,注释为钙离子结合蛋白。这些蛋白通过影响信号转导、矿物质转运调节、胁迫反应、细胞壁稳定性、种子发育和酶活性调节,在调节小麦籽粒中矿物质积累方面发挥着重要作用。值得注意的是,与携带 C 等位基因的品种相比,携带 T 等位基因的品种表现出更高的镁、磷和钙在小麦籽粒中的积累,这表明携带 T 等位基因的品种在小麦面板中得到了积极的选择。因此,探索这些蛋白质的作用机制和调控方法,可有助于提高小麦籽粒的营养价值,特别是矿物质含量。
Targeting candidate genes for the macronutrient accumulation of wheat grains for improved human nutrition
Wheat biofortification represents a promising avenue to combat global micronutrient deficiencies. Therefore, understanding the genetic basis of desirable alleles underpinning all minerals is important for wheat biofortification. A core collection of 111 wheat genotypes was evaluated for both years to measure macronutrient contents, including magnesium (Mg), calcium (Ca), phosphorus (P), and potassium (K). Our study showed significant natural phenotypic variation for all of the studied macronutrients underlying both years with high heritability values. Using a genome-wide association scan (GWAS), 474 significant SNP markers were detected and associated with all the evaluated macronutrients for both years. Upon GWAS analysis, several reliable genomic regions with different potential candidate genes were found to play essential roles in the biofortified wheat grains. For instance, the gene TraesCS1A02G261200 was located on chromosome 1A and annotated as calcium ion binding proteins. These proteins play a crucial role in regulating mineral accumulation in wheat grains by influencing signal transduction, mineral transporter regulation, stress responses, cell wall stability, seed development, and enzymatic activity modulation. Notably, the accessions carrying T allele showed a higher accumulation of Mg, P, and Ca in wheat grains than the accessions carrying C allele, suggesting the positive selection of the accessions carrying T allele in the wheat panel. Therefore, exploring the mechanisms and regulation of these proteins could lead to techniques for enhancing the nutritional value of wheat grains, specifically mineral content.
期刊介绍:
This journal publishes original papers presenting new scientific results on breeding, genetics, physiology, pathology and production of primarily wheat, rye, barley, oats and maize.