Aleksey Kachalkin, Aleksandra Bekkarevich, Maria Tomashevskaya, Anna Glushakova
{"title":"桦木中长角甲幼虫（Cerambycidae）粪便中的酵母菌以及 Diddensiella monakovoensis f.a., sp.","authors":"Aleksey Kachalkin, Aleksandra Bekkarevich, Maria Tomashevskaya, Anna Glushakova","doi":"10.1007/s11756-024-01770-x","DOIUrl":null,"url":null,"abstract":"<p>In the study of yeasts from frass of longhorn beetle larvae, species with high abundance from the genera <i>Diddensiella</i><i>, </i><i>Scheffersomyces</i> and <i>Sugiyamaella</i> and some others with lower abundance were observed<i>.</i> The novel ascomycetous yeast species <i>Diddensiella monakovoensis</i> f.a., sp. nov. (holotype: KBP Y-6906; isotypes: VKM Y-3676 and DBVPG 8067) is proposed based on a conventional DNA-barcode sequence analysis and physiological characteristics. The new yeast species differs by 3.5% (11 nt substitutions and 7 indels) in the internal transcribed spacer (ITS region) of the rDNA sequence from the type strain <i>D. caesifluorescens</i> NCAIM Y.01949<sup> T</sup> and has about 2% (11 nt substitutions) and 9% (76 nt substitutions and 4 indels) differences from it by sequences of the partial large-subunit (LSU) region and translation elongation factor 1-alpha (<i>TEF1-α</i>) gene, respectively. The genetic differences between the species are also underpinned by different physiology.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"27 1","pages":""},"PeriodicalIF":1.4000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Yeasts from frass of longhorn beetle larvae (Cerambycidae) in birch wood and description of Diddensiella monakovoensis f.a., sp. nov\",\"authors\":\"Aleksey Kachalkin, Aleksandra Bekkarevich, Maria Tomashevskaya, Anna Glushakova\",\"doi\":\"10.1007/s11756-024-01770-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>In the study of yeasts from frass of longhorn beetle larvae, species with high abundance from the genera <i>Diddensiella</i><i>, </i><i>Scheffersomyces</i> and <i>Sugiyamaella</i> and some others with lower abundance were observed<i>.</i> The novel ascomycetous yeast species <i>Diddensiella monakovoensis</i> f.a., sp. nov. (holotype: KBP Y-6906; isotypes: VKM Y-3676 and DBVPG 8067) is proposed based on a conventional DNA-barcode sequence analysis and physiological characteristics. The new yeast species differs by 3.5% (11 nt substitutions and 7 indels) in the internal transcribed spacer (ITS region) of the rDNA sequence from the type strain <i>D. caesifluorescens</i> NCAIM Y.01949<sup> T</sup> and has about 2% (11 nt substitutions) and 9% (76 nt substitutions and 4 indels) differences from it by sequences of the partial large-subunit (LSU) region and translation elongation factor 1-alpha (<i>TEF1-α</i>) gene, respectively. The genetic differences between the species are also underpinned by different physiology.</p>\",\"PeriodicalId\":8978,\"journal\":{\"name\":\"Biologia\",\"volume\":\"27 1\",\"pages\":\"\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-08-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11756-024-01770-x\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biologia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11756-024-01770-x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
在对长角甲虫幼虫粪便中酵母菌的研究中，观察到了 Diddensiella 属、Scheffersomyces 属和 Sugiyamaella 属中含量较高的菌种，以及其他一些含量较低的菌种。根据常规 DNA 条形码序列分析和生理特征，提出了新的子囊酵母菌 Diddensiella monakovoensis f.a.，sp. nov.（主模式：KBP Y-6906；等模式：VKM Y-3676 和 DBVPG 8067）。该酵母新种在 rDNA 序列的内部转录间隔区（ITS 区）与模式菌株 D. caesifluorescens NCAIM Y.01949 T 的差异为 3.5%（11 nt 置换），在部分大亚基（LSU）区和翻译伸长因子 1-α（TEF1-α）基因序列上与模式菌株 D. caesifluorescens NCAIM Y.01949 T 的差异分别约为 2%（11 nt 置换）和 9%（76 nt 置换和 4 个缺口）。物种之间的遗传差异还受到不同生理机能的支撑。
Yeasts from frass of longhorn beetle larvae (Cerambycidae) in birch wood and description of Diddensiella monakovoensis f.a., sp. nov
In the study of yeasts from frass of longhorn beetle larvae, species with high abundance from the genera Diddensiella, Scheffersomyces and Sugiyamaella and some others with lower abundance were observed. The novel ascomycetous yeast species Diddensiella monakovoensis f.a., sp. nov. (holotype: KBP Y-6906; isotypes: VKM Y-3676 and DBVPG 8067) is proposed based on a conventional DNA-barcode sequence analysis and physiological characteristics. The new yeast species differs by 3.5% (11 nt substitutions and 7 indels) in the internal transcribed spacer (ITS region) of the rDNA sequence from the type strain D. caesifluorescens NCAIM Y.01949 T and has about 2% (11 nt substitutions) and 9% (76 nt substitutions and 4 indels) differences from it by sequences of the partial large-subunit (LSU) region and translation elongation factor 1-alpha (TEF1-α) gene, respectively. The genetic differences between the species are also underpinned by different physiology.
期刊介绍：
Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
