从花生壳上生长的链霉菌中分离和鉴定具有抗菌活性的新型邻苯二甲酸酯衍生物

IF 1.4 4区 生物学 Q3 BIOLOGY
Fatma H. Motawe, Mohamed A. M. El Gendy, Nadia H. Abd El-Nasser, Hemaia M. Motawe
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引用次数: 0

摘要

病原体的抗菌药耐药性是导致全球数百万人死亡的原因。因此,本研究的目的是从链霉菌中鉴定和分离出一种新的抗菌剂,利用农业废弃物对多种病原微生物(包括耐多药(MDR)病原体)有效。研究人员从埃及土壤中分离、鉴定了链霉菌,并在当地的农业废弃物上进行了培养。利用井扩散法测试了发酵培养基对几种病原体的抗菌活性。确定了最适合生产抗菌生物活性化合物的发酵和环境因素。最后,对生物活性化合物进行了纯化,并利用多项色谱和仪器分析对其进行了表征。我们确定了在花生壳废物上生长的链霉菌 Bingchenggensis FHM200 分离菌株。在温度为 28-30℃ 和 pH 值为 7.5-8 的条件下,用 2%(体积分数)的分离菌株(105 个孢子/毫升)培养 5 天,并辅以 0.2%(体积分数)的硝酸钾和磷酸氢二钾(1 克/升),可获得最高的抗菌活性。最后,我们成功分离并纯化了一种新型生物活性化合物:邻苯二甲酸(2-羟基己基)己酯,它对所有测试病原体都具有良好的抗菌活性,对蜡样芽孢杆菌 ATCC 33018 的 MIC 为 70 µg/mL,对黑曲霉 NRRL 326 的 MIC 为 150 µg/mL,对 MDR 病原体的 MIC 为 2500 µg/mL;耐甲氧西林金黄色葡萄球菌(MRSA)ATCC 43300 为 2500 µg/mL,大肠杆菌 797 和白色念珠菌 210 为 3000 µg/mL。我们得出结论,从冰城链霉菌 FHM200 中分离出的邻苯二甲酸己酯(2-羟基己酯)具有良好的抗菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Isolation and identification of a novel phthalate derivative from Streptomyces sp. grown on peanut shells with antimicrobial activity

Isolation and identification of a novel phthalate derivative from Streptomyces sp. grown on peanut shells with antimicrobial activity

Antimicrobial resistance to pathogens is the cause of several million deaths globally. Therefore, the aim of the current study is to identify and isolate a new antimicrobial agent from Streptomyces sp. effective against several pathogenic microbes, including multidrug resistant (MDR) pathogens, using agriculture waste. Streptomyces isolate strain from the Egyptian soil was isolated, identified and grown on a local agricultural waste. The antimicrobial activity of the fermentation medium was tested against several pathogens using the well diffusion method. The most suitable fermentation and environmental factors for the maximum production of the antimicrobial bioactive compound was determined. Finally, the bioactive compound was purified, and characterized using several chromatographic and instrumental analyses. We identified Streptomyces bingchenggensis FHM200 isolate strain grown on peanut shells wastes. The maximum antimicrobial activity was attained by incubation with 2% (v/v) of the isolated strain (105 spores / mL) at temperature range of 28–30℃ and pH range of 7.5 to 8 for 5 days, supplemented with potassium nitrate 0.2% (w/v) and dipotassium hydrogen phosphate (1 g/L). Finally, we managed to isolate and purify a novel bioactive compound; hexyl (2-hydroxyhexyl) phthalate, that possessed a promising antimicrobial activity against all tested pathogens with MIC of 70 µg/mL for Bacillus cereus ATCC 33018, 150 µg/mL for Aspergillus niger NRRL 326, and for MDR pathogens; 2500 µg/mL for methicillin resistance Staphylococcus aureus (MRSA) ATCC 43300, and 3000 µg/mL for both Escherichia coli 797, and Candida albicans 210. We concluded that hexyl (2-hydroxyhexyl) phthalate, isolated from Streptomyces bingchenggensis FHM200, possesses promising antimicrobial activity.

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来源期刊
Biologia
Biologia 生物-生物学
CiteScore
3.30
自引率
6.70%
发文量
290
审稿时长
6 months
期刊介绍: Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.
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