对西班牙养猪场土葡萄球菌分离物毒性和抗菌性的深入了解

Oscar Mencía-Ares, Eva Ramos-Calvo, Alba González-Fernández, Álvaro Aguarón-Turrientes, Ana Isabel Pastor-Calonge, Rubén Miguélez-Pérez, César B. Gutiérrez-Martín, Sonia Martínez-Martínez
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引用次数: 0

摘要

土葡萄球菌是猪的一种重要病原体,主要引起渗出性表皮炎。要解决猪葡萄球菌感染问题,就必须同时确定猪场分离物的毒力和抗菌药耐药性(AMR)。本研究旨在分析西班牙猪场分离的 S. hyicus 的毒力、AMR 和生物膜形成的特征。共分析了 49 个分离株,它们来自出现皮肤、生殖和全身临床症状的动物。半数分离株(49.0%)至少有一种毒力因子(VF)基因呈阳性,其中 SHETA 基因最常见(28.6%)。观察发现,耐多药(MDR)分离株的频率很高(83.7%),对常用抗菌药的耐药性很强,包括林可酰胺类(83.7%)、胸膜霉素类(81.6%)、青霉素类(75.5%)和四环素类(73.5%)。所有分离物均表现出强大的体外生物膜形成能力(DC = 15.6 ± 7.0)。在VFs、生物膜形成和AMR模式之间发现了显著的关联,突出了对林可酰胺类和胸腺嘧啶类(p < 0.001; Φ = 0.57)和大环内酯类(p < 0.001; Φ = 0.48)耐药性之间的联系,以及AMR与ExhC和ExhD VF基因之间的关联。这些研究结果表明,有必要进行有针对性的诊断,以改善管理和治疗策略,减轻猪丹毒对猪生产的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Insights into the Virulence and Antimicrobial Resistance of Staphylococcus hyicus Isolates from Spanish Swine Farms
Staphylococcus hyicus is a significant pathogen in swine, primarily causing exudative epidermitis. Addressing S. hyicus infections requires both the characterization of virulence and antimicrobial resistance (AMR) in farm-recovered isolates. This study aimed to characterize the virulence, AMR, and biofilm formation of S. hyicus isolates from Spanish swine farms. A total of 49 isolates were analyzed, originating from animals with cutaneous, reproductive, and systemic clinical signs. Half of the isolates (49.0%) were positive for at least one virulence factor (VF) gene, with SHETA being the most frequent (28.6%). A high frequency of multidrug resistant (MDR) isolates was observed (83.7%), with significant resistance to commonly used antimicrobials, including lincosamides (83.7%), pleuromutilins (81.6%), penicillins (75.5%), and tetracyclines (73.5%). All isolates exhibited robust in vitro biofilm formation capacity (DC = 15.6 ± 7.0). Significant associations were found between VFs, biofilm formation, and AMR patterns, highlighting the link between the resistance to lincosamides and pleuromutilins (p < 0.001; Φ = 0.57) and macrolides (p < 0.001; Φ = 0.48), and the association of AMR with the ExhC and ExhD VF genes. These findings underscore the need for targeted diagnostics to improve management and therapeutic strategies to mitigate the impact of S. hyicus on swine production.
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