噬菌体 PEF1 中两种新型内溶酶的特征及其对控制粪肠球菌浮游细胞和生物膜细胞的综合效果评估

Chen Wang, Junxin Zhao, Yunzhi Lin, Su Zar Chi Lwin, Mohamed El-Telbany, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto
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引用次数: 0

摘要

内溶酶是一种源于噬菌体的溶菌酶,已成为一种很有前途的替代抗菌剂,可用于抗击日益增多的耐多药细菌感染。我们克隆了两种新型内溶酶 LysPEF1-1 和 LysPEF1-2,它们来自肠球菌噬菌体 PEF1,并在大肠杆菌中进行了过表达,以测试它们对耐多药粪肠球菌菌株及其生物膜的抗菌效果。LysPEF1-1 包含一个酶活性结构域和一个细胞壁结合结构域,分别来自 NLPC-P60 和 SH3 超家族,而 LysPEF1-2 则包含一个属于 PGRP 超家族的假定肽聚糖识别结构域。与噬菌体 PEF1 相比,LysPEF1-1 对 89.86%(62/69)的受试肠球菌具有活性,显示出更广的抗菌谱。此外,两种内溶酶体对氯仿预处理的其他革兰氏阳性和革兰氏阴性菌种具有溶菌活性。与 LysPEF1-2 相比,LysPEF1-1 对耐多药粪肠球菌 E5 菌株的活性更高。与单独使用相比,两种内溶素的组合能有效减少肉汤中 E5 的浮游细胞,并能更有效地抑制生物膜的形成和清除粪肠球菌 JCM 7783T 的生物膜细胞。特别是在 4 °C条件下,在玻璃载玻片表面处理 2 小时后,它们能将存活的生物膜细胞减少 4.5 log。结果表明,这两种新型内溶素可作为控制粪肠球菌感染的替代抗菌剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Two Novel Endolysins from Bacteriophage PEF1 and Evaluation of Their Combined Effects on the Control of Enterococcus faecalis Planktonic and Biofilm Cells
Endolysin, a bacteriophage-derived lytic enzyme, has emerged as a promising alternative antimicrobial agent against rising multidrug-resistant bacterial infections. Two novel endolysins LysPEF1-1 and LysPEF1-2 derived from Enterococcus phage PEF1 were cloned and overexpressed in Escherichia coli to test their antimicrobial efficacy against multidrug-resistant E. faecalis strains and their biofilms. LysPEF1-1 comprises an enzymatically active domain and a cell-wall-binding domain originating from the NLPC-P60 and SH3 superfamilies, while LysPEF1-2 contains a putative peptidoglycan recognition domain that belongs to the PGRP superfamily. LysPEF1-1 was active against 89.86% (62/69) of Enterococcus spp. tested, displaying a wider antibacterial spectrum than phage PEF1. Moreover, two endolysins demonstrated lytic activity against additional gram-positive and gram-negative species pretreated with chloroform. LysPEF1-1 showed higher activity against multidrug-resistant E. faecalis strain E5 than LysPEF1-2. The combination of two endolysins effectively reduced planktonic cells of E5 in broth and was more efficient at inhibiting biofilm formation and removing biofilm cells of E. faecalis JCM 7783T than used individually. Especially at 4 °C, they reduced viable biofilm cells by 4.5 log after 2 h of treatment on glass slide surfaces. The results suggest that two novel endolysins could be alternative antimicrobial agents for controlling E. faecalis infections.
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