哈茨真菌挥发性有机化合物对造成采后蛇果黑腐病的 Thielaviopsis paradoxa 的生物防治作用

IF 2.9 4区 综合性期刊 Q1 Multidisciplinary
Toga Pangihotan Napitupulu, Des Saputro Wibowo, Muhammad Ilyas
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引用次数: 0

摘要

本研究的目的是通过生物探究各种毛霉菌株的挥发性有机化合物(VOCs)来控制采后蛇果的黑腐病,蛇果是东南亚的一种重要水果商品,由Thielaviopsis paradoxa真菌引起。通过间接对抗试验发现,T. harzianum InaCC F88 是抑制作用最强的菌株。该菌株对 T. paradoxa 的抑制率为 71.14%。利用顶空气相色谱-质谱(Headspace GC-MS)对该菌株进行的挥发性分析表明,最丰富的挥发性有机化合物是异戊醇(36.06%),其次是 2-甲基-1-丙醇(21.92%)和 2-环戊烯酮(10.72%)。在加入 10、20 和 30 µL 的纯化合物蒸气后,异戊醇作为主要化合物对 T. paradoxa 的抑制率分别为 71.44%、28.88% 和 2.86%。此外,在 1.5 升密闭容器试验中,加入 300 µL 异戊醇蒸气后,在室温下贮藏 7 天后,感染前果实的病变组织可减少 29.15%,而在没有纯化合物的情况下,病变组织可减少 58.97%。总之,T. harzianum InaCC F88 通过其挥发性有机化合物具有生物防治蛇果黑腐病的潜力,从而延长了蛇果的贮藏时间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biocontrol of Thielaviopsis paradoxa Causing Black Rot on Postharvest Snake Fruit by Volatile Organic Compounds of Trichoderma harzianum

Biocontrol of Thielaviopsis paradoxa Causing Black Rot on Postharvest Snake Fruit by Volatile Organic Compounds of Trichoderma harzianum

The purpose of this study was to bioprospect the volatile organic compounds (VOCs) of various Trichoderma harzianum strains to control black rot of postharvest snake fruit, an important fruit commodity in Southeast Asia, caused by the fungus Thielaviopsis paradoxa. Trough an indirect confrontation assay, T. harzianum InaCC F88 was found as the most suppressing strain among others. The strain inhibited T. paradoxa with growth relative to control (GRC) of 71.14%. A volatolomic analysis using Headspace GC–MS of this strain showed the most abundant VOC was isoamyl alcohol (36.06%), followed by 2-methyl-1-propanol (21.92%) and 2-cyclopentenone (10.72%). Isoamyl alcohol as the major compound inhibited T. paradoxa with GRC of 71.44, 28.88, and 2.86% after the addition of 10, 20, and 30 µL of the vapor of pure compound, respectively. Moreover, in a 1.5-L close-container assay, the addition of 300 µL isoamyl alcohol vapor was also able to reduce lesion tissue in the pre-infected fruit up to 29.15% after 7 days of storage in room temperature compared to 58.97% in the absence of the pure compound. In conclusion, T. harzianum InaCC F88 through its VOCs was potential to biocontrol black rot in snake fruit, thus extend its storage time.

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来源期刊
Arabian Journal for Science and Engineering
Arabian Journal for Science and Engineering 综合性期刊-综合性期刊
CiteScore
5.20
自引率
3.40%
发文量
0
审稿时长
4.3 months
期刊介绍: King Fahd University of Petroleum & Minerals (KFUPM) partnered with Springer to publish the Arabian Journal for Science and Engineering (AJSE). AJSE, which has been published by KFUPM since 1975, is a recognized national, regional and international journal that provides a great opportunity for the dissemination of research advances from the Kingdom of Saudi Arabia, MENA and the world.
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