小鼠固有活化浆细胞树突状细胞的淋巴来源

IF 6.4 1区生物学 Q1 BIOLOGY

eLife Pub Date : 2024-09-13 DOI:https://doi.org/10.7554/elife.96394.3

Alessandra Machado Araujo, Joseph D Dekker, Kendra Garrison, Zhe Su, Catherine Rhee, Zicheng Hu, Bum-Kyu Lee, Daniel Osorio, Jiwon Lee, Vishwanath R Iyer, Lauren IR Ehrlich, George Georgiou, Gregory Ippolito, Stephen Yi, Haley O Tucker

{"title":"小鼠固有活化浆细胞树突状细胞的淋巴来源","authors":"Alessandra Machado Araujo, Joseph D Dekker, Kendra Garrison, Zhe Su, Catherine Rhee, Zicheng Hu, Bum-Kyu Lee, Daniel Osorio, Jiwon Lee, Vishwanath R Iyer, Lauren IR Ehrlich, George Georgiou, Gregory Ippolito, Stephen Yi, Haley O Tucker","doi":"https://doi.org/10.7554/elife.96394.3","DOIUrl":null,"url":null,"abstract":"We identified a novel mouse plasmacytoid dendritic cell (pDC) lineage derived from the common lymphoid progenitors (CLPs) that is dependent on expression of Bcl11a. These CLP-derived pDCs, which we refer to as ‘B-pDCs’, have a unique gene expression profile that includes hallmark B cell genes, normally not expressed in conventional pDCs. Despite expressing most classical pDC markers such as SIGLEC-H and PDCA1, B-pDCs lack IFN-α secretion, exhibiting a distinct inflammatory profile. Functionally, B-pDCs induce T cell proliferation more robustly than canonical pDCs following Toll-like receptor 9 (TLR9) engagement. B-pDCs, along with another homogeneous subpopulation of myeloid-derived pDCs, display elevated levels of the cell surface receptor tyrosine kinase AXL, mirroring human AXL+ transitional DCs in function and transcriptional profile. Murine B-pDCs therefore represent a phenotypically and functionally distinct CLP-derived DC lineage specialized in T cell activation and previously not described in mice.","PeriodicalId":11640,"journal":{"name":"eLife","volume":null,"pages":null},"PeriodicalIF":6.4000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Lymphoid origin of intrinsically activated plasmacytoid dendritic cells in mice\",\"authors\":\"Alessandra Machado Araujo, Joseph D Dekker, Kendra Garrison, Zhe Su, Catherine Rhee, Zicheng Hu, Bum-Kyu Lee, Daniel Osorio, Jiwon Lee, Vishwanath R Iyer, Lauren IR Ehrlich, George Georgiou, Gregory Ippolito, Stephen Yi, Haley O Tucker\",\"doi\":\"https://doi.org/10.7554/elife.96394.3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We identified a novel mouse plasmacytoid dendritic cell (pDC) lineage derived from the common lymphoid progenitors (CLPs) that is dependent on expression of Bcl11a. These CLP-derived pDCs, which we refer to as ‘B-pDCs’, have a unique gene expression profile that includes hallmark B cell genes, normally not expressed in conventional pDCs. Despite expressing most classical pDC markers such as SIGLEC-H and PDCA1, B-pDCs lack IFN-α secretion, exhibiting a distinct inflammatory profile. Functionally, B-pDCs induce T cell proliferation more robustly than canonical pDCs following Toll-like receptor 9 (TLR9) engagement. B-pDCs, along with another homogeneous subpopulation of myeloid-derived pDCs, display elevated levels of the cell surface receptor tyrosine kinase AXL, mirroring human AXL+ transitional DCs in function and transcriptional profile. Murine B-pDCs therefore represent a phenotypically and functionally distinct CLP-derived DC lineage specialized in T cell activation and previously not described in mice.\",\"PeriodicalId\":11640,\"journal\":{\"name\":\"eLife\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.4000,\"publicationDate\":\"2024-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"eLife\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/https://doi.org/10.7554/elife.96394.3\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"eLife","FirstCategoryId":"99","ListUrlMain":"https://doi.org/https://doi.org/10.7554/elife.96394.3","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

我们发现了一种新型小鼠浆细胞树突状细胞（pDC）系，这种细胞系来源于普通淋巴祖细胞（CLPs），依赖于 Bcl11a 的表达。这些由 CLP 衍生的 pDC（我们称之为 "B-pDC"）具有独特的基因表达谱，其中包括 B 细胞标志基因，这些基因通常不在传统 pDC 中表达。尽管 B-pDCs 表达大多数经典的 pDC 标记，如 SIGLEC-H 和 PDCA1，但它们缺乏 IFN-α 分泌，表现出独特的炎症特征。在功能上，B-pDC 与典型 pDC 相比，在 Toll 样受体 9（TLR9）参与后能更强有力地诱导 T 细胞增殖。B-pDCs与另一个同质的髓源性pDCs亚群一起显示出细胞表面受体酪氨酸激酶AXL水平的升高，在功能和转录特征上与人类AXL+过渡性DCs相似。因此，小鼠 B-pDCs 代表了一种表型和功能上与众不同的 CLP 派生 DC 系，专门用于 T 细胞活化，以前未在小鼠中描述过。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Lymphoid origin of intrinsically activated plasmacytoid dendritic cells in mice

We identified a novel mouse plasmacytoid dendritic cell (pDC) lineage derived from the common lymphoid progenitors (CLPs) that is dependent on expression of Bcl11a. These CLP-derived pDCs, which we refer to as ‘B-pDCs’, have a unique gene expression profile that includes hallmark B cell genes, normally not expressed in conventional pDCs. Despite expressing most classical pDC markers such as SIGLEC-H and PDCA1, B-pDCs lack IFN-α secretion, exhibiting a distinct inflammatory profile. Functionally, B-pDCs induce T cell proliferation more robustly than canonical pDCs following Toll-like receptor 9 (TLR9) engagement. B-pDCs, along with another homogeneous subpopulation of myeloid-derived pDCs, display elevated levels of the cell surface receptor tyrosine kinase AXL, mirroring human AXL⁺ transitional DCs in function and transcriptional profile. Murine B-pDCs therefore represent a phenotypically and functionally distinct CLP-derived DC lineage specialized in T cell activation and previously not described in mice.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

eLife BIOLOGY-

CiteScore

12.90

自引率

3.90%

发文量

3122

审稿时长

17 weeks

期刊介绍： eLife is a distinguished, not-for-profit, peer-reviewed open access scientific journal that specializes in the fields of biomedical and life sciences. eLife is known for its selective publication process, which includes a variety of article types such as: Research Articles: Detailed reports of original research findings. Short Reports: Concise presentations of significant findings that do not warrant a full-length research article. Tools and Resources: Descriptions of new tools, technologies, or resources that facilitate scientific research. Research Advances: Brief reports on significant scientific advancements that have immediate implications for the field. Scientific Correspondence: Short communications that comment on or provide additional information related to published articles. Review Articles: Comprehensive overviews of a specific topic or field within the life sciences.