{"title":"利用灰度级和色彩通道评估方法分析生物斑点模式","authors":"Srivathsav Karnati and Biswajit Pathak","doi":"10.1088/1555-6611/ad7720","DOIUrl":null,"url":null,"abstract":"Biospeckle offers a practical tool for contact-free testing and monitoring of biological samples, providing unique insights into dynamics of biological processes. In the present work, we design an experimental arrangement to perform quality assessment on biological samples using biospeckle patterns. We analyse the speckle patterns and evaluate its important parameters by constructing a grey-level co-occurrence matrix (GLCM). Furthermore, we propose an alternative and reliable method to study the biospeckle patterns by constructing a color-channel assessment matrix. The proposed approach provides both qualitative and quantitative information of the sample under study, with minimum speckle images and no stringent requirement of correct parameter selection, unlike in the case of GLCM method. Proof-of-concept experimental results are provided that demonstrate the feasibility of the proposed method in evaluating the quality of biological samples.","PeriodicalId":17976,"journal":{"name":"Laser Physics","volume":"6 1","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of biospeckle pattern using grey-level and color-channel assessment methods\",\"authors\":\"Srivathsav Karnati and Biswajit Pathak\",\"doi\":\"10.1088/1555-6611/ad7720\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Biospeckle offers a practical tool for contact-free testing and monitoring of biological samples, providing unique insights into dynamics of biological processes. In the present work, we design an experimental arrangement to perform quality assessment on biological samples using biospeckle patterns. We analyse the speckle patterns and evaluate its important parameters by constructing a grey-level co-occurrence matrix (GLCM). Furthermore, we propose an alternative and reliable method to study the biospeckle patterns by constructing a color-channel assessment matrix. The proposed approach provides both qualitative and quantitative information of the sample under study, with minimum speckle images and no stringent requirement of correct parameter selection, unlike in the case of GLCM method. Proof-of-concept experimental results are provided that demonstrate the feasibility of the proposed method in evaluating the quality of biological samples.\",\"PeriodicalId\":17976,\"journal\":{\"name\":\"Laser Physics\",\"volume\":\"6 1\",\"pages\":\"\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Laser Physics\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://doi.org/10.1088/1555-6611/ad7720\",\"RegionNum\":4,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"OPTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Laser Physics","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1088/1555-6611/ad7720","RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"OPTICS","Score":null,"Total":0}
Analysis of biospeckle pattern using grey-level and color-channel assessment methods
Biospeckle offers a practical tool for contact-free testing and monitoring of biological samples, providing unique insights into dynamics of biological processes. In the present work, we design an experimental arrangement to perform quality assessment on biological samples using biospeckle patterns. We analyse the speckle patterns and evaluate its important parameters by constructing a grey-level co-occurrence matrix (GLCM). Furthermore, we propose an alternative and reliable method to study the biospeckle patterns by constructing a color-channel assessment matrix. The proposed approach provides both qualitative and quantitative information of the sample under study, with minimum speckle images and no stringent requirement of correct parameter selection, unlike in the case of GLCM method. Proof-of-concept experimental results are provided that demonstrate the feasibility of the proposed method in evaluating the quality of biological samples.
期刊介绍:
Laser Physics offers a comprehensive view of theoretical and experimental laser research and applications. Articles cover every aspect of modern laser physics and quantum electronics, emphasizing physical effects in various media (solid, gaseous, liquid) leading to the generation of laser radiation; peculiarities of propagation of laser radiation; problems involving impact of laser radiation on various substances and the emerging physical effects, including coherent ones; the applied use of lasers and laser spectroscopy; the processing and storage of information; and more.
The full list of subject areas covered is as follows:
-physics of lasers-
fibre optics and fibre lasers-
quantum optics and quantum information science-
ultrafast optics and strong-field physics-
nonlinear optics-
physics of cold trapped atoms-
laser methods in chemistry, biology, medicine and ecology-
laser spectroscopy-
novel laser materials and lasers-
optics of nanomaterials-
interaction of laser radiation with matter-
laser interaction with solids-
photonics