利用灰度级和色彩通道评估方法分析生物斑点模式

IF 1.2 4区 物理与天体物理 Q4 OPTICS
Srivathsav Karnati and Biswajit Pathak
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引用次数: 0

摘要

生物斑纹为生物样本的非接触式测试和监测提供了一种实用工具,为生物过程的动态提供了独特的见解。在本研究中,我们设计了一种实验安排,利用生物斑点模式对生物样本进行质量评估。我们通过构建灰度级共现矩阵 (GLCM) 分析斑点模式并评估其重要参数。此外,我们还提出了另一种可靠的方法,即通过构建颜色通道评估矩阵来研究生物斑点模式。与 GLCM 方法不同的是,所提出的方法只需最小的斑点图像,对参数的正确选择没有严格要求,就能提供所研究样本的定性和定量信息。概念验证实验结果证明了所提方法在评估生物样本质量方面的可行性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of biospeckle pattern using grey-level and color-channel assessment methods
Biospeckle offers a practical tool for contact-free testing and monitoring of biological samples, providing unique insights into dynamics of biological processes. In the present work, we design an experimental arrangement to perform quality assessment on biological samples using biospeckle patterns. We analyse the speckle patterns and evaluate its important parameters by constructing a grey-level co-occurrence matrix (GLCM). Furthermore, we propose an alternative and reliable method to study the biospeckle patterns by constructing a color-channel assessment matrix. The proposed approach provides both qualitative and quantitative information of the sample under study, with minimum speckle images and no stringent requirement of correct parameter selection, unlike in the case of GLCM method. Proof-of-concept experimental results are provided that demonstrate the feasibility of the proposed method in evaluating the quality of biological samples.
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来源期刊
Laser Physics
Laser Physics 物理-光学
CiteScore
2.60
自引率
8.30%
发文量
127
审稿时长
2.2 months
期刊介绍: Laser Physics offers a comprehensive view of theoretical and experimental laser research and applications. Articles cover every aspect of modern laser physics and quantum electronics, emphasizing physical effects in various media (solid, gaseous, liquid) leading to the generation of laser radiation; peculiarities of propagation of laser radiation; problems involving impact of laser radiation on various substances and the emerging physical effects, including coherent ones; the applied use of lasers and laser spectroscopy; the processing and storage of information; and more. The full list of subject areas covered is as follows: -physics of lasers- fibre optics and fibre lasers- quantum optics and quantum information science- ultrafast optics and strong-field physics- nonlinear optics- physics of cold trapped atoms- laser methods in chemistry, biology, medicine and ecology- laser spectroscopy- novel laser materials and lasers- optics of nanomaterials- interaction of laser radiation with matter- laser interaction with solids- photonics
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