淀粉芽孢杆菌 W82T-44 对大豆生长的促进作用及其对大豆疫霉根腐病的生物防治潜力

IF 2.3 3区农林科学 Q1 AGRONOMY

Plant Pathology Pub Date : 2024-09-11 DOI:10.1111/ppa.13994

Haixu Liu, Zhuoqun Zhang, Ying Xu, Xiuming Du, Jingzhi Wen

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引用次数: 0

摘要

大豆最具破坏性的土传病害是由疫霉（Phytophthora sojae）引起的疫霉根腐病（PRR）。生物防治已成为减少土传病害的有效方法。本研究从抗病大豆品种 Williams 82 的根瘤土壤中分离出细菌菌株 W82T-44。经鉴定，该菌株为淀粉芽孢杆菌（Bacillus amyloliquefaciens），对 P. sojae 菌丝生长的抑制率为 92.4%。W82T-44 产生纤维素酶、苷元酶和蛋白酶，能显著促进大豆生长。与阴性对照相比，W82T-44 能显著增加大豆的株高（22.34 厘米对 18.47 厘米）、全株鲜重（2.57 克对 2.05 克）、干重（0.43 克对 0.32 克）、根系总长度（266.80 厘米对 182.53 厘米）、根系面积（27.10 平方厘米对 17.84 平方厘米）和根系体积（1.88 立方厘米对 1.16 立方厘米）。W82T-44 的发酵滤液对 P. sojae 的菌丝生长、卵孢子形成和子囊萌发有显著的抑制作用，此外还能促进子囊孢子的胞囊化。W82T-44 具有参与脂肽合成的基因，包括杆菌素、表面活性素、芬吉霉素和杆菌霉素。此外，它还能显著上调大豆根系中的致病相关基因（β-1,3-葡聚糖酶、致病相关基因 1 的非表达因子、几丁质酶）和编码防御酶的基因（多酚氧化酶、苯丙氨酸氨化酶、过氧化物酶）（p < 0.05）。用 W82T-44 株系处理的大豆的 PRR 疾病指数（11.67）显著低于阴性对照（40.00；p <；0.05）。本研究从多个角度表明了淀粉芽孢杆菌 W82T-44 作为生物防治剂防治 PRR 的潜力。

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The growth‐promoting effects of Bacillus amyloliquefaciens W82T‐44 on soybean and its biocontrol potential against soybean Phytophthora root rot

The most devastating soilborne disease of soybean is Phytophthora root rot (PRR) caused by Phytophthora sojae. Biological control has emerged as an effective method of reducing soilborne diseases. The present study isolated the bacterial strain W82T‐44 from the rhizosphere soil of a resistant soybean variety Williams 82. This strain was identified as Bacillus amyloliquefaciens and exhibited a 92.4% inhibition of mycelial growth of P. sojae. W82T‐44 produced cellulase, siderophore and protease and significantly promoted soybean growth. W82T‐44 treatment, compared to the negative control, was found to significantly (p < 0.05) increase soybean plant height (22.34 vs. 18.47 cm), fresh weight of the whole plant (2.57 vs. 2.05 g), dry weight (0.43 vs. 0.32 g), total root length (266.80 vs. 182.53 cm), root area (27.10 vs. 17.84 cm2) and root volume (1.88 vs. 1.16 cm3). The fermentation filtrate of W82T‐44 had significant inhibitory effects on mycelial growth, oospore formation and cyst germination in P. sojae and additionally promoted zoospore encystment. W82T‐44 possessed genes involved in lipopeptide synthesis, including bacilysin, surfactin, fengycin and bacillomycin. Moreover, it significantly upregulated pathogenesis‐related genes (β‐1,3‐glucanase, nonexpressor of pathogenesis‐related gene 1, chitinase) and genes encoding defence enzymes (polyphenol oxidase, phenylalanine ammonia‐lyase, peroxidase) in soybean roots (p < 0.05). The PRR disease index of soybean treated with the W82T‐44 strain was significantly lower (11.67) than the negative control (40.00; p < 0.05). The present study indicates the potential of B. amyloliquefaciens W82T‐44 as a biological control agent against PRR from various perspectives.

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来源期刊

Plant Pathology 生物-农艺学

CiteScore

5.60

自引率

7.40%

发文量

147

审稿时长

3 months

期刊介绍： This international journal, owned and edited by the British Society for Plant Pathology, covers all aspects of plant pathology and reaches subscribers in 80 countries. Top quality original research papers and critical reviews from around the world cover: diseases of temperate and tropical plants caused by fungi, bacteria, viruses, phytoplasmas and nematodes; physiological, biochemical, molecular, ecological, genetic and economic aspects of plant pathology; disease epidemiology and modelling; disease appraisal and crop loss assessment; and plant disease control and disease-related crop management.