与豹纹珊瑚石斑鱼(Plectropomus leopardus)定居过渡期有关的有氧代谢变化

IF 2.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY
Adam T. Downie, Caroline M. Phelps, Björn Illing, Jen Whan, Mark I. McCormick, Jodie L. Rummer
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引用次数: 0

摘要

变态是珊瑚礁鱼类生态学的一个重要方面。这一发育里程碑标志着形态和功能的变化,使浮游幼体成功过渡到珊瑚礁上的底栖生活方式。然而,我们对这一时期发生的生理变化知之甚少,特别是与游泳相关的能量的潜在变化。这一点至关重要,因为游泳是浮游幼体找到合适珊瑚礁定居的机制。当珊瑚石斑鱼(石斑鱼科:豹纹石斑鱼)幼体在夜间来到一个边缘礁附近定居时,对其进行采集,并对其生理变态过程进行描述。以与生态相关的游泳速度对幼体和定居 24 小时的幼体进行耐力游泳测试,并测量活动期间的摄氧量。为了描述组织的有氧和无氧特性在变态过程中的变化,我们还分别测量了全身柠檬酸合成酶和乳酸脱氢酶的活性,以及躯干和胸鳍的线粒体密度。我们的方法精确测量了这些生命阶段在招募过程中所需的摄氧量,尽管幼体的游泳速度每秒仅快 1.5 个体长(BLs-1),但其质量特异性摄氧量(ṀO2)却比定居的幼体高出 74%。柠檬酸合成酶活性在沉降时明显下降;幼体的柠檬酸合成酶活性是幼体的 3.7 倍,这表明组织有氧代谢的快速变化可能是该物种变态过程中的一个重要过程。相比之下,乳酸脱氢酶在沉降时没有明显差异。这些发现突显了中上层珊瑚石斑鱼幼体在 24 小时内的一些生理变化,这些变化有助于成功地在珊瑚礁上定居。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Changes in aerobic metabolism associated with the settlement transition for the leopard coral grouper (Plectropomus leopardus)

Changes in aerobic metabolism associated with the settlement transition for the leopard coral grouper (Plectropomus leopardus)

Metamorphosis is a critical aspect of coral reef fish ecology. This developmental milestone marks changes in form and function that permit successful transition of pelagic larvae to the demersal lifestyle on coral reefs. However, we know very little about the physiological changes that occur during this period, specifically potential changes in energetics associated with swimming. This is critical, as swimming is the mechanism by which pelagic larvae find a suitable reef on which to settle. Coral grouper larvae (Serranidae: Plectropomas leopardus) were collected at night as they came into the vicinity of a fringing reef to settle, and their physiological metamorphosis was characterized. Larvae and 24 h-settled juveniles were exposed to an endurance swimming test at ecologically relevant swimming speeds, and oxygen uptake rates were measured during activity. To describe how aerobic and anaerobic properties of tissues change during metamorphosis, we also measured whole body citrate synthase and lactate dehydrogenase activity, respectively, as well as mitochondrial density in the trunk and pectoral fins. Our approach accurately measures the oxygen uptake rates these life stages need during the recruitment process, with larvae having a 74% higher mass-specific oxygen uptake rate (O2) than settled juveniles despite swimming at speeds that are only 1.5 body-lengths per second (BLs−1) faster. Citrate synthase activity significantly decreased upon settlement; as larvae had 3.7 times higher activities than juveniles, suggesting that rapid changes in aerobic metabolism of tissues may be an important process during metamorphosis in this species. In contrast, lactate dehydrogenase did not significantly differ upon settlement. These findings highlight some physiological modifications that pelagic coral grouper larvae undertake within 24 h that contribute to successfully settling onto a coral reef.

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来源期刊
Coral Reefs
Coral Reefs 生物-海洋与淡水生物学
CiteScore
6.80
自引率
11.40%
发文量
111
审稿时长
4-8 weeks
期刊介绍: Coral Reefs, the Journal of the International Coral Reef Society, presents multidisciplinary literature across the broad fields of reef studies, publishing analytical and theoretical papers on both modern and ancient reefs. These encourage the search for theories about reef structure and dynamics, and the use of experimentation, modeling, quantification and the applied sciences. Coverage includes such subject areas as population dynamics; community ecology of reef organisms; energy and nutrient flows; biogeochemical cycles; physiology of calcification; reef responses to natural and anthropogenic influences; stress markers in reef organisms; behavioural ecology; sedimentology; diagenesis; reef structure and morphology; evolutionary ecology of the reef biota; palaeoceanography of coral reefs and coral islands; reef management and its underlying disciplines; molecular biology and genetics of coral; aetiology of disease in reef-related organisms; reef responses to global change, and more.
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