自然光和 LED 光条件下珊瑚 Acropora digitifera 蛋白素基因的昼夜表达模式

IF 2.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY
Zongyan Shi, Ee Suan Tan, Akihiro Takemura
{"title":"自然光和 LED 光条件下珊瑚 Acropora digitifera 蛋白素基因的昼夜表达模式","authors":"Zongyan Shi, Ee Suan Tan, Akihiro Takemura","doi":"10.1007/s00338-024-02558-w","DOIUrl":null,"url":null,"abstract":"<p>Photoreception is essential to coral growth, reproduction, and stress responses. Thus far, opsin-based photoreception and potential photoadaptation in Scleractinian corals remains unclear. This study used natural and light-emitting diode (LED) lighting to investigate how <i>Acropora digitifera</i>, which is adapted to shallow-water environments, responds to day–night conditions. We successfully cloned three opsin genes (<i>Adopsin1, Adopsin2</i>, and <i>Adopsin3</i>)<i>. Adopsin1</i> and <i>Adopsin2</i> clustered with the Cnidopsins, whereas <i>Adopsin3</i> clustered with the anthozoan-specific opsin I group. In situ hybridization showed positive signals of these genes in coral endodermal and ectodermal layers. When <i>A. digitifera</i> branches were reared under a day–night cycle with natural light, a day-high and night-low pattern was observed in the transcript levels of <i>Adopsin1</i> and <i>Adopsin3.</i> Genes related to calcification [plasma membrane calcium transporting ATPase 2 (<i>PMCA</i>)] and oxygen homeostasis regulation [hypoxia-inducible factor 1 alpha (<i>HIF1α</i>)] showed similar patterns. Rearing of branches under a day–night cycle (photoperiod = 12:12, 26.5–29.3 μmol s<sup>−1</sup> m<sup>−2</sup>) with red (<i>λ</i><sub>max</sub> = 628 nm), but not blue (464 nm) or green (519 nm) LED lighting led to increases in transcript levels of <i>Adopsin1</i> and <i>Adopsin3</i> during photophase. The transcript levels of carbonic anhydrase, <i>PMCA</i>, <i>HIFα</i>, and sodium-glucose cotransporter were significantly higher during photophase than during scotophase. Furthermore, <i>Adopsin3</i> upregulation occurred within 4 h of exposure to a red LED light at night. These results suggest that <i>A. digitifera</i> can responding to long wavelengths of light, which play a crucial role in the photophysiology of the coral host. The capacity to perceive red light provides advantages in physiological adaptation and ecological niche occupation by <i>A. digitifera</i> in shallow waters.</p>","PeriodicalId":10821,"journal":{"name":"Coral Reefs","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Day–night expression patterns of opsin genes in the coral Acropora digitifera under natural and LED light conditions\",\"authors\":\"Zongyan Shi, Ee Suan Tan, Akihiro Takemura\",\"doi\":\"10.1007/s00338-024-02558-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Photoreception is essential to coral growth, reproduction, and stress responses. Thus far, opsin-based photoreception and potential photoadaptation in Scleractinian corals remains unclear. This study used natural and light-emitting diode (LED) lighting to investigate how <i>Acropora digitifera</i>, which is adapted to shallow-water environments, responds to day–night conditions. We successfully cloned three opsin genes (<i>Adopsin1, Adopsin2</i>, and <i>Adopsin3</i>)<i>. Adopsin1</i> and <i>Adopsin2</i> clustered with the Cnidopsins, whereas <i>Adopsin3</i> clustered with the anthozoan-specific opsin I group. In situ hybridization showed positive signals of these genes in coral endodermal and ectodermal layers. When <i>A. digitifera</i> branches were reared under a day–night cycle with natural light, a day-high and night-low pattern was observed in the transcript levels of <i>Adopsin1</i> and <i>Adopsin3.</i> Genes related to calcification [plasma membrane calcium transporting ATPase 2 (<i>PMCA</i>)] and oxygen homeostasis regulation [hypoxia-inducible factor 1 alpha (<i>HIF1α</i>)] showed similar patterns. Rearing of branches under a day–night cycle (photoperiod = 12:12, 26.5–29.3 μmol s<sup>−1</sup> m<sup>−2</sup>) with red (<i>λ</i><sub>max</sub> = 628 nm), but not blue (464 nm) or green (519 nm) LED lighting led to increases in transcript levels of <i>Adopsin1</i> and <i>Adopsin3</i> during photophase. The transcript levels of carbonic anhydrase, <i>PMCA</i>, <i>HIFα</i>, and sodium-glucose cotransporter were significantly higher during photophase than during scotophase. Furthermore, <i>Adopsin3</i> upregulation occurred within 4 h of exposure to a red LED light at night. These results suggest that <i>A. digitifera</i> can responding to long wavelengths of light, which play a crucial role in the photophysiology of the coral host. The capacity to perceive red light provides advantages in physiological adaptation and ecological niche occupation by <i>A. digitifera</i> in shallow waters.</p>\",\"PeriodicalId\":10821,\"journal\":{\"name\":\"Coral Reefs\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-09-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Coral Reefs\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00338-024-02558-w\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Coral Reefs","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00338-024-02558-w","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

光感知对珊瑚的生长、繁殖和应激反应至关重要。迄今为止,硬骨鱼类珊瑚基于蛋白的光感知和潜在的光适应仍不清楚。本研究利用自然光和发光二极管(LED)照明来研究适应浅水环境的数字珊瑚(Acropora digitifera)如何对昼夜条件做出反应。我们成功克隆了三个眼色素基因(Adopsin1、Adopsin2 和 Adopsin3)。Adopsin1 和 Adopsin2 与栉水母蛋白(Cnidopsins)聚类,而 Adopsin3 则与无脊椎动物特异性蛋白 I 组聚类。原位杂交显示,这些基因在珊瑚的内胚层和外胚层呈阳性信号。在昼夜循环的自然光照下饲养数码珊瑚枝条时,观察到 Adopsin1 和 Adopsin3 的转录水平呈昼高夜低的模式。与钙化相关的基因[质膜钙转运ATP酶2(PMCA)]和氧稳态调节相关的基因[缺氧诱导因子1α(HIF1α)]也表现出类似的模式。在昼夜循环(光周期 = 12:12,26.5-29.3 μmol s-1 m-2)条件下,用红色(λmax = 628 nm)而非蓝色(464 nm)或绿色(519 nm)LED光照饲养枝条,会导致光相期 Adopsin1 和 Adopsin3 的转录水平增加。碳酸酐酶、PMCA、HIFα和钠-葡萄糖共转运体的转录水平在光照阶段明显高于光照阶段。此外,Adopsin3的上调发生在夜间暴露于红色LED光的4小时内。这些结果表明,A. digitifera 能够对长波长的光做出反应,这在珊瑚宿主的光生理学中起着至关重要的作用。对红光的感知能力为A. digitifera在浅水区的生理适应和生态位占据提供了优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Day–night expression patterns of opsin genes in the coral Acropora digitifera under natural and LED light conditions

Day–night expression patterns of opsin genes in the coral Acropora digitifera under natural and LED light conditions

Photoreception is essential to coral growth, reproduction, and stress responses. Thus far, opsin-based photoreception and potential photoadaptation in Scleractinian corals remains unclear. This study used natural and light-emitting diode (LED) lighting to investigate how Acropora digitifera, which is adapted to shallow-water environments, responds to day–night conditions. We successfully cloned three opsin genes (Adopsin1, Adopsin2, and Adopsin3). Adopsin1 and Adopsin2 clustered with the Cnidopsins, whereas Adopsin3 clustered with the anthozoan-specific opsin I group. In situ hybridization showed positive signals of these genes in coral endodermal and ectodermal layers. When A. digitifera branches were reared under a day–night cycle with natural light, a day-high and night-low pattern was observed in the transcript levels of Adopsin1 and Adopsin3. Genes related to calcification [plasma membrane calcium transporting ATPase 2 (PMCA)] and oxygen homeostasis regulation [hypoxia-inducible factor 1 alpha (HIF1α)] showed similar patterns. Rearing of branches under a day–night cycle (photoperiod = 12:12, 26.5–29.3 μmol s−1 m−2) with red (λmax = 628 nm), but not blue (464 nm) or green (519 nm) LED lighting led to increases in transcript levels of Adopsin1 and Adopsin3 during photophase. The transcript levels of carbonic anhydrase, PMCA, HIFα, and sodium-glucose cotransporter were significantly higher during photophase than during scotophase. Furthermore, Adopsin3 upregulation occurred within 4 h of exposure to a red LED light at night. These results suggest that A. digitifera can responding to long wavelengths of light, which play a crucial role in the photophysiology of the coral host. The capacity to perceive red light provides advantages in physiological adaptation and ecological niche occupation by A. digitifera in shallow waters.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Coral Reefs
Coral Reefs 生物-海洋与淡水生物学
CiteScore
6.80
自引率
11.40%
发文量
111
审稿时长
4-8 weeks
期刊介绍: Coral Reefs, the Journal of the International Coral Reef Society, presents multidisciplinary literature across the broad fields of reef studies, publishing analytical and theoretical papers on both modern and ancient reefs. These encourage the search for theories about reef structure and dynamics, and the use of experimentation, modeling, quantification and the applied sciences. Coverage includes such subject areas as population dynamics; community ecology of reef organisms; energy and nutrient flows; biogeochemical cycles; physiology of calcification; reef responses to natural and anthropogenic influences; stress markers in reef organisms; behavioural ecology; sedimentology; diagenesis; reef structure and morphology; evolutionary ecology of the reef biota; palaeoceanography of coral reefs and coral islands; reef management and its underlying disciplines; molecular biology and genetics of coral; aetiology of disease in reef-related organisms; reef responses to global change, and more.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信