叠加 Hi-C：为识别单细胞染色体相互作用而提出的解决方案

arXiv - QuanBio - Genomics Pub Date : 2024-08-23 DOI:arxiv-2408.13039

Jia Zhang, Li Xiao, Peng Qi, Yaling Zeng, Xumeng Chen, Duan-fang Liao, Kai Li

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引用次数: 0

摘要

Hi-C 测序被广泛用于分析染色体相互作用。在本研究中，我们提出了 "叠加Hi-C"，其特点是在连接子中加入成对的EcoP15I位点，以促进与目标DNA的粘端连接。叠加Hi-C克服了Hi-C的技术局限性，可鉴定单个细胞的染色体相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Superimposed Hi-C: A Solution Proposed for Identifying Single Cell's Chromosomal Interactions

Hi-C sequencing is widely used for analyzing chromosomal interactions. In this study, we propose "superimposed Hi-C" which features paired EcoP15I sites in a linker to facilitate sticky-end ligation with target DNAs. Superimposed Hi-C overcomes Hi-C's technical limitations, enabling the identification of single cell's chromosomal interactions.

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arXiv - QuanBio - Genomics

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