基于过氧化物酶样纳米酶的微环境响应型可注射水凝胶用于细菌感染伤口的愈合

IF 4.4 2区 工程技术 Q1 ENGINEERING, MULTIDISCIPLINARY
Wei Zhou, XiaNan Li, MengCheng Tang, LingFeng Pan, BeiBei Wang, Hao Zhang, Han Zhang, ShiBo Wang, XiangDong Kong
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引用次数: 0

摘要

水凝胶具有出色的保湿性能和吸收伤口渗出物的能力,是最有吸引力的伤口敷料之一。然而,传统的水凝胶敷料往往缺乏对微环境的反应能力,仅仅充当伤口的保护屏障。因此,它们在预防感染和促进伤口修复方面的效果有限。为了解决这些问题,我们开发了一种基于过氧化物酶类纳米酶的多功能可注射水凝胶 CF/MS@HG,旨在快速愈合细菌感染的伤口。该水凝胶主要由氧化海藻酸钠、胺化明胶和聚赖氨酸组成,包裹有 MIL-101(CuFe) 纳米粒子(CF)和硒化锰纳米粒子(MnSe2 NPs,或 MS NPs)。注射后,复合物通过希夫碱反应在感染伤口部位迅速凝胶化。体外实验证明,这种水凝胶具有很强的粘附性和自愈能力。此外,CF 还具有类似过氧化物酶 (POD) 的活性,能在伤口微环境中催化原位过氧化氢 (H2O2) 生成剧毒的羟基自由基 (-OH),诱导细菌氧化损伤。同时,MS 在微酸性伤口微环境中分解成 H2Se,破坏细菌的新陈代谢并抑制其增殖。聚赖氨酸的加入进一步增强了水凝胶的抗菌性能。体内实验表明,这种水凝胶具有极佳的生物安全性,能显著促进伤口愈合。这种多功能智能水凝胶在治疗细菌感染伤口方面大有可为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Peroxidase-like nanozyme based microenvironment-responsive injectable hydrogel for bacteria-infected wound healing

Hydrogel stands out as one of the most attractive wound dressings due to its excellent moisturizing properties and capacity to absorb wound exudates. However, conventional hydrogel dressings often lack responsiveness to the microenvironment, merely acting as protective barriers for the wound. Consequently, they exhibit limited effectiveness in preventing infection and facilitating wound repair. To address these problems, we have developed a multifunctional injectable hydrogel, CF/MS@HG, based on peroxidase-like nanozymes, aiming at rapidly healing bacterial-infected wounds. The hydrogel is mainly composed of oxidized sodium alginate, aminated gelatin, and polylysine, encapsulating MIL-101(CuFe) NPs (CF) and manganese selenide nanoparticles (MnSe2 NPs, or MS NPs). After injection, the complex rapidly gelatinizes at the infected wound site through a Schiff base reaction. In vitro experiments have demonstrated the hydrogel’s strong adhesion and self-healing capabilities. Moreover, CF exhibiting peroxidase (POD)-like activity, catalyzes in situ hydrogen peroxide (H2O2) to generate highly toxic hydroxyl radicals (·OH) within the wound microenvironment, inducing oxidative damage to bacteria. Meanwhile, MS decomposes into H2Se in the slightly acidic wound microenvironment, disrupting bacterial metabolism and inhibiting proliferation. The addition of polylysine further enhances the hydrogel’s antibacterial properties. In vivo experiments have shown that the hydrogel exhibits excellent biological safety and significantly promotes wound healing. This multifunctional smart hydrogel holds great promise for the treatment of bacterial-infected wounds.

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来源期刊
Science China Technological Sciences
Science China Technological Sciences ENGINEERING, MULTIDISCIPLINARY-MATERIALS SCIENCE, MULTIDISCIPLINARY
CiteScore
8.40
自引率
10.90%
发文量
4380
审稿时长
3.3 months
期刊介绍: Science China Technological Sciences, an academic journal cosponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China, and published by Science China Press, is committed to publishing high-quality, original results in both basic and applied research. Science China Technological Sciences is published in both print and electronic forms. It is indexed by Science Citation Index. Categories of articles: Reviews summarize representative results and achievements in a particular topic or an area, comment on the current state of research, and advise on the research directions. The author’s own opinion and related discussion is requested. Research papers report on important original results in all areas of technological sciences. Brief reports present short reports in a timely manner of the latest important results.
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