烧伤创面对糖萼失调的影响

Hannes Kühtreiber, Daniel Bormann, Melanie Salek, Thomas Haider, Caterina Selina Mildner, Marie-Therese Lingitz, Clemens Aigner, Christine Radtke, Hendrik Jan Ankersmit, Michael Mildner
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引用次数: 0

摘要

烧伤通常会导致严重的并发症,包括急性呼吸窘迫综合征(ARDS),部分原因是全身炎症和糖萼破坏。在这项研究中,我们分析了 28 名烧伤患者的血清,并利用转录组分析来解读烧伤对糖萼片失调的影响。我们观察到免疫细胞衍生的降解酶,尤其是基质金属蛋白酶-8(MMP8)明显上调,这与免疫细胞浸润增加和糖萼失调有关。烧伤患者的血清分析显示,脱落的糖萼成分和 MMP8 水平明显升高,两者都与吸入性损伤有关。因此,用 MMP8 处理人类体外肺组织模型可诱导内皮和上皮细胞中的糖萼明显脱落。我们的这些数据表明,MMP8 是造成糖萼破坏和烧伤后肺损伤的一个因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The impact of burn trauma on glycocalyx derangement
Burn injuries often lead to severe complications, including acute respiratory distress syndrome (ARDS), driven in part by systemic inflammation and glycocalyx disruption. In this study, we analyzed the sera of 28 patients after burn trauma and utilized transcriptomic analyses to decipher the impact of burn injury on glycocalyx derangement. We observed significant upregulation of immune cell-derived degrading enzymes, particularly matrix metalloproteinase-8 (MMP8), which correlated with increased immune cell infiltration and glycocalyx derangement. Serum analysis of burn patients revealed significantly elevated levels of shed glycocalyx components and MMP8, both correlating with the presence of inhalation injury. Consequently, treatment of human in vitro lung tissue models with MMP8 induced significant glycocalyx shedding in both, the endothelium and epithelium. Together our data suggest MMP8 as a contributor of glycocalyx disruption and lung injury post-burn.
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