揭示 Lathyrus 物种的生殖生物学、物候学和花粉活力,促进作物改良

IF 1.6 3区 农林科学 Q2 AGRONOMY
Muthusamy Shankar, Ravi Gowthami, Kuldeep Tripathi, Doddamalur Appaji Deepak, Kerur Vishwanath Raghavendra, Anuradha Agrawal
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引用次数: 0

摘要

由于对 Lathyrus 的生殖生物学了解有限,通过遗传改良和栽培品种开发提高 Lathyrus 的发展面临挑战。因此,本研究系统地研究了 Lathyrus sativus L. 和 L. cicera L. 的生殖生物学、物候学、柱头接受性、发芽和花粉活力。分别通过显微镜观察和过氧化氢测试确定了花药开裂和柱头接受能力。花粉活力通过体外花粉萌发和二醋酸荧光素(FDA)测试进行评估。研究发现,花朵为两性花和原花柱,花朵的清花性有利于自花授粉。两个物种都有五个花发育阶段和三个豆荚发育阶段。在所研究的品种中,所有阶段的时间差异都很大。据观察,柱头接受能力出现在花药开裂前 2-4 天,并一直持续到花枯萎。这项研究揭示了L. sativus和L. cicera主要是自花授粉,强调了在育种过程中成功人工杂交的关键雄蕊形成时间,最终提高结荚率和结籽率,促进植物育种人员优化杂交计划。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unveiling reproductive biology, phenology, and pollen viability in Lathyrus species to enhance crop improvement

Unveiling reproductive biology, phenology, and pollen viability in Lathyrus species to enhance crop improvement

The advancement of Lathyrus through genetic enhancement and cultivar development faces challenges due to limited knowledge about their reproductive biology. Thus, the present study systematically investigates the reproductive biology, phenology, receptivity of stigma, germination and viability of pollen in Lathyrus sativus L. and L. cicera L. Experiments were carried out using 20 accessions of L. sativus and three accessions of L. cicera. Anther dehiscence and stigma receptivity were determined through microscopic observation and hydrogen peroxide test, respectively. Pollen viability was assessed through in vitro pollen germination and fluorescein diacetate (FDA) test. Flowers were found to be bisexual and protogynous, with self-pollination favored by the cleistogamous nature of flowers. Five flower-development and three pod-development stages were classified in both species. The timing of all stages differed significantly among the accessions studied. Stigma receptivity was observed to occur 2–4 days before anther dehiscence and persisted until flower wilting. The study reveals that L. sativus and L. cicera are primarily self-pollinated, underscoring the critical timing of emasculation for successful manual crossing in breeding, ultimately enhancing pod and seed set, and facilitating plant breeders in optimizing hybridization programs.

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来源期刊
Genetic Resources and Crop Evolution
Genetic Resources and Crop Evolution 农林科学-农艺学
CiteScore
4.10
自引率
10.00%
发文量
178
审稿时长
3 months
期刊介绍: Genetic Resources and Crop Evolution is devoted to all aspects of plant genetic resources research. It publishes original articles in the fields of taxonomical, morphological, physiological, biochemical, genetical, cytological or ethnobotanical research of genetic resources and includes contributions to gene-bank management in a broad sense, that means to collecting, maintenance, evaluation, storage and documentation. Areas of particular interest include: -crop evolution -domestication -crop-weed relationships -related wild species -history of cultivated plants including palaeoethnobotany. Genetic Resources and Crop Evolution also publishes short communications, e.g. newly described crop taxa, nomenclatural notes, reports of collecting missions, evaluation results of gene-bank material etc. as well as book reviews of important publications in the field of genetic resources. Every volume will contain some review articles on actual problems. The journal is the internationalized continuation of the German periodical Die Kulturpflanze, published formerly by the Institute of Plant Genetics and Crop Plant Research at Gatersleben, Germany. All contributions are in the English language and are subject to peer reviewing.
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