针对海湾战争病小鼠模型中功能失调的内源性大麻素信号转导。

IF 4.6 2区 医学 Q1 NEUROSCIENCES
Erica Squire , Hye-Lim Lee , Woojin Jeong , Sumin Lee , V. Ravichandiran , Charles L. Limoli , Daniele Piomelli , Vipan Kumar Parihar , Kwang-Mook Jung
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引用次数: 0

摘要

海湾战争疾病(GWI)是一种慢性疾病,其特征是一系列不同的症状,包括疼痛、疲劳、焦虑和认知障碍。这些症状被认为是由于在不可预测的压力下暴露于海湾战争(GW)的有毒化学物质(包括杀虫剂、神经毒剂和预防性药物)所造成的损害。我们假设,海湾战争综合症的发病机制可能源于内源性大麻素(ECB)系统的长期破坏,该系统是一种信号复合体,在大脑中发挥着重要的保护功能。通过使用小鼠 GWI 模型,我们发现与健康对照组相比,患病小鼠大脑中 ECB 信使--anandamide--的组织水平显著降低。此外,在 GWI 小鼠的前额叶皮层和大脑小胶质细胞中,脂肪酸酰胺水解酶(FAAH)的编码基因 Faah 的转录也明显升高。服用 FAAH 抑制剂 URB597 后,这些动物表现出的行为缺陷得到了纠正,包括焦虑样和抑郁样行为增强,以及恐惧记忆消失缺陷,从而使脑内的苯甲酰胺水平恢复正常。此外,GWI 小鼠的小胶质细胞转录组也出现了意想不到的变化,这意味着免疫刺激会持续抑制同态监控基因和促炎基因的异常表达。这些结果表明,暴露于 GW 化学物质会导致大脑 ECB 信号的缺失,而这种缺失与小胶质细胞功能的持续改变有关。通过药物使安乃近介导的 ECB 信号转导正常化,可为改善 GWI 症状提供有效的治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Targeting dysfunctional endocannabinoid signaling in a mouse model of Gulf War illness

Targeting dysfunctional endocannabinoid signaling in a mouse model of Gulf War illness

Gulf War Illness (GWI) is a chronic disorder characterized by a heterogeneous set of symptoms that include pain, fatigue, anxiety, and cognitive impairment. These are thought to stem from damage caused by exposure under unpredictable stress to toxic Gulf War (GW) chemicals, which include pesticides, nerve agents, and prophylactic drugs. We hypothesized that GWI pathogenesis might be rooted in long-lasting disruption of the endocannabinoid (ECB) system, a signaling complex that serves important protective functions in the brain. Using a mouse model of GWI, we found that tissue levels of the ECB messenger, anandamide, were significantly reduced in the brain of diseased mice, compared to healthy controls. In addition, transcription of the Faah gene, which encodes for fatty acid amide hydrolase (FAAH), the enzyme that deactivates anandamide, was significant elevated in prefrontal cortex of GWI mice and brain microglia. Behavioral deficits exhibited by these animals, including heightened anxiety-like and depression-like behaviors, and defective extinction of fearful memories, were corrected by administration of the FAAH inhibitor, URB597, which normalized brain anandamide levels. Furthermore, GWI mice displayed unexpected changes in the microglial transcriptome, implying persistent dampening of homeostatic surveillance genes and abnormal expression of pro-inflammatory genes upon immune stimulation. Together, these results suggest that exposure to GW chemicals produce a deficit in brain ECB signaling which is associated with persistent alterations in microglial function. Pharmacological normalization of anandamide-mediated ECB signaling may offer an effective therapeutic strategy for ameliorating GWI symptomology.

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来源期刊
Neuropharmacology
Neuropharmacology 医学-神经科学
CiteScore
10.00
自引率
4.30%
发文量
288
审稿时长
45 days
期刊介绍: Neuropharmacology publishes high quality, original research and review articles within the discipline of neuroscience, especially articles with a neuropharmacological component. However, papers within any area of neuroscience will be considered. The journal does not usually accept clinical research, although preclinical neuropharmacological studies in humans may be considered. The journal only considers submissions in which the chemical structures and compositions of experimental agents are readily available in the literature or disclosed by the authors in the submitted manuscript. Only in exceptional circumstances will natural products be considered, and then only if the preparation is well defined by scientific means. Neuropharmacology publishes articles of any length (original research and reviews).
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