经尿道活检/切除标本组织学检查后福尔马林溶液上清液的细胞学评估。

Ying Wang, Tamera J Strzepek, Dawn M Costello, Patricia A Crowley, John S Plavnicky, Donna K Russell, Guan Wu, Jerome Jean-Gilles, Hiroshi Miyamoto
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引用次数: 0

摘要

背景经尿道活检标本中出现的尿路上皮变性偶尔可能表明存在盘状高级别尿路上皮癌(HGUC):目的:确定从经尿道活检/切除标本容器中收集的福尔马林溶液上清液中是否能检测到变性尿路细胞:我们对福尔马林上清液进行了细胞块(n = 43)或薄层涂片(ThinPrep)(n = 57)处理评估:在细胞块组中,43 例病例中只有 2 例(5%)(1 例 pTa HGUC,1 例 pT1 HGUC)出现罕见的尿道细胞。相比之下,在 ThinPrep 方法中,57 个病例中有 52 个(91%)的涂片评估结果令人满意。12 例 pTa/pT1 病例中有 7 例(58%)和 9 例 pTis 病例中有 6 例(67%)的涂片通过细胞学诊断为 HGUC。值得注意的是,在 5 例组织学诊断为尿路上皮不典型性的病例中,有 2 例(40%)检测到 HGUC 细胞,怀疑为尿路上皮原位癌,但不能确诊。此外,在 31 个病例中,苏木精-伊红染色的切片显示尿道变性但无明确癌变,其中有 HGUC 细胞(31 例中有 2 例;6%)、非典型尿道细胞(31 例中有 5 例;16%)或良性尿道细胞(31 例中有 20 例;65%),31 例中只有 4 例(13%)不合格:结论:从福尔马林上清液中提取 ThinPrep 涂片进行细胞学检查,即使是在将整个经尿道活检/切除标本提交组织学检查之后,也有助于评估变性的尿路上皮细胞。这项技术尤其适用于出现广泛尿路上皮变性的非肿瘤性病例,以检测可能的恶性细胞和/或不确定病例,帮助做出更明确的诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytologic Assessment of the Supernatants of Formalin Solution Following Histologic Examination of Transurethral Biopsy/Resection Specimens.

Context.—: Urothelial denudation seen in transurethral biopsy specimens may occasionally indicate the presence of discohesive high-grade urothelial carcinoma (HGUC).

Objective.—: To determine if denuded urothelial cells can be detected in the supernatants of formalin solution collected from the containers of transurethral biopsy/resection specimens after the entire tissue was submitted for histologic examination.

Design.—: We assessed the formalin supernatants by processing for cell block (n = 43) or ThinPrep smear (n = 57).

Results.—: In the cell block cohort, only 2 of 43 cases (5%) (1 pTa HGUC, 1 pT1 HGUC) showed rare urothelial cells. By contrast, in the ThinPrep method, the smear was satisfactory for evaluation in 52 of 57 cases (91%). The cytologic diagnosis of HGUC was made in the smears from 7 of 12 (58%) pTa/pT1 cases and 6 of 9 (67%) pTis cases. Remarkably, HGUC cells were detected in 2 of 5 cases (40%) with histologic diagnosis of urothelial atypia suspicious for but not diagnostic of urothelial carcinoma in situ. Additionally, in 31 cases exhibiting urothelial denudation without definitive cancer on hematoxylin-eosin-stained slides, HGUC cells (2 of 31; 6%), atypical urothelial cells (5 of 31; 16%), or benign-appearing urothelial cells (20 of 31; 65%) were present, and only 4 of 31 (13%) were unsatisfactory.

Conclusions.—: Cytologic examination of ThinPrep smears from the formalin supernatants even following submission of the entire transurethral biopsy/resection specimens for histologic examination is useful for assessing denuded urothelial cells. This technique can particularly be applied to nonneoplastic cases showing extensive urothelial denudation to detect possible malignant cells and/or indeterminate cases to assist to make a more definitive diagnosis.

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