将内质网应激调节伴侣作为特发性非梗阻性无精子症生物标志物的研究。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Cigdem Cicek , Pelin Telkoparan-Akillilar , Semra Sertyel , Cumhur Bilgi , Osman Denizhan Ozgun
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引用次数: 0

摘要

无精子症是指男性射出的精液中完全没有精子细胞。通常情况下,精子是在睾丸中产生的,并受到一系列复杂的细胞和分子相互作用的调节。当细胞内的内质网(ER)正常功能发生偏离或损坏时,就会产生内质网应激。为了应对这种压力,一连串的反应机制被激活,以调节细胞内的内质网压力。本研究旨在探讨内质网(ER)应激调节伴侣作为男性不育症潜在生物标志物的作用。与无精子症相关的ER应激可在睾丸中的精原细胞等细胞中表现出来,并可影响精子的生成。ER应激会导致细胞内多种蛋白质的表达和活性发生改变。这些蛋白质包括调节ER应激反应的伴侣蛋白。经计算,每组样本量至少为 36 名患者。在这项初步研究中,我们测量并比较了特发性非梗阻性无精子症男性受试者与非不育男性对照组之间血清中蛋白二硫化物异构酶 A1(PDI1)、蛋白二硫化物异构酶 A3(PDIA3)、间脑星形胶质细胞源性神经营养因子(MANF)、葡萄糖调节蛋白 78(GRP78)、集束蛋白(CLU)、钙网蛋白(CRT)和钙粘连蛋白(CNX)的水平。血清 PDIA1(p=0.0004)、MANF(p=0.018)、PDIA3(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of endoplasmic reticulum stress-regulated chaperones as biomarkers in idiopathic nonobstructive azoospermia

Azoospermia is a condition in which sperm cells are completely absent in a male's ejaculate. Typically, sperm production occurs in the testes and is regulated by a complex series of cellular and molecular interactions. Endoplasmic reticulum (ER) stress arises when there is a deviation from or damage to the normal functions of the ER within cells. In response to this stress, a cascade of response mechanisms is activated to regulate ER stress within cells. This study aims to investigate the role of ER stress-regulated chaperones as potential biomarkers in male infertility. ER stress associated with azoospermia can manifest in cells such as spermatogonia in the testes and can impact sperm production. As a result of ER stress, the expression and activity of a variety of proteins within cells can be altered. Among these proteins are chaperone proteins that regulate the ER stress response. The sample size was calculated to be a minimum of 36 patients in each group. In this preliminary study, we measured and compared serum levels of protein disulfide-isomerase A1, protein disulfide-isomerase A3 (PDIA3), mesencephalic astrocyte-derived neurotrophic factor (MANF), glucose regulatory protein 78 (GRP78), clusterin (CLU), calreticulin (CRT), and calnexin (CNX) between male subjects with idiopathic nonobstructive azoospermia and a control group of noninfertile males. Serum PDIA1 (P = 0.0004), MANF (P = 0.018), PDIA3 (P < 0.0001), GRP78 (P = 0.0027), and CRT (P = 0.0009) levels were higher in the infertile group compared to the control. In summary, this study presents novel findings in a cohort of male infertile patients, emphasizing the significance of incorporating diverse biomarkers. It underscores the promising role of ER stress-regulated proteins as potential serum indicators for male infertility. By elucidating the impact of ER stress on spermatogenic cells, the research illuminates the maintenance or disruption of cellular health. A deeper understanding of these results could open the door to novel treatment approaches for reproductive conditions, including azoospermia.

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来源期刊
Cell Stress & Chaperones
Cell Stress & Chaperones 生物-细胞生物学
CiteScore
7.60
自引率
2.60%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Cell Stress and Chaperones is an integrative journal that bridges the gap between laboratory model systems and natural populations. The journal captures the eclectic spirit of the cellular stress response field in a single, concentrated source of current information. Major emphasis is placed on the effects of climate change on individual species in the natural environment and their capacity to adapt. This emphasis expands our focus on stress biology and medicine by linking climate change effects to research on cellular stress responses of animals, micro-organisms and plants.
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