PICKLE介导的核小体缩聚驱动H3K27me3扩散,从而在分化过程中继承多角体记忆

IF 14.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhenwei Liang, Tao Zhu, Yaoguang Yu, Caihong Wu, Yisui Huang, Yuanhao Hao, Xin Song, Wei Fu, Liangbing Yuan, Yuhai Cui, Shangzhi Huang, Chenlong Li
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引用次数: 0

摘要

在动物和植物中,H3K27me3的扩散对于维持有丝分裂可遗传的多角体介导的染色质沉默至关重要。然而,Polycomb抑制复合体2(PRC2)如何在扩散区域获得未修饰的核小体以扩散H3K27me3仍不清楚。在这里,我们在拟南芥中发现,染色质重塑因子 PICKLE(PKL)在 H3K27me3 扩增过程中发挥着特殊作用,以保护分化过程中的细胞特性。PKL特异性地定位在H3K27me3扩散区域,而不是成核位点,并与PRC2发生物理结合。PKL的缺失会破坏PRC2催化亚基CLF在扩增区域的占据,并导致异常的去分化。PKL的ATP酶功能提高了核小体密度,确保了PRC2催化活性可以获得未修饰的核小体,以进行H3K27me3扩增。我们的研究结果表明,依赖于PKL的核小体压实对于PRC2在H3K27me3扩散过程中介导的H3K27me3读写功能至关重要,从而揭示了抑制性染色质域的建立和传播机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

PICKLE-mediated nucleosome condensing drives H3K27me3 spreading for the inheritance of Polycomb memory during differentiation

PICKLE-mediated nucleosome condensing drives H3K27me3 spreading for the inheritance of Polycomb memory during differentiation

Spreading of H3K27me3 is crucial for the maintenance of mitotically inheritable Polycomb-mediated chromatin silencing in animals and plants. However, how Polycomb repressive complex 2 (PRC2) accesses unmodified nucleosomes in spreading regions for spreading H3K27me3 remains unclear. Here, we show in Arabidopsis thaliana that the chromatin remodeler PICKLE (PKL) plays a specialized role in H3K27me3 spreading to safeguard cell identity during differentiation. PKL specifically localizes to H3K27me3 spreading regions but not to nucleation sites and physically associates with PRC2. Loss of PKL disrupts the occupancy of the PRC2 catalytic subunit CLF in spreading regions and leads to aberrant dedifferentiation. Nucleosome density increase endowed by the ATPase function of PKL ensures that unmodified nucleosomes are accessible to PRC2 catalytic activity for H3K27me3 spreading. Our findings demonstrate that PKL-dependent nucleosome compaction is critical for PRC2-mediated H3K27me3 read-and-write function in H3K27me3 spreading, thus revealing a mechanism by which repressive chromatin domains are established and propagated.

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来源期刊
Molecular Cell
Molecular Cell 生物-生化与分子生物学
CiteScore
26.00
自引率
3.80%
发文量
389
审稿时长
1 months
期刊介绍: Molecular Cell is a companion to Cell, the leading journal of biology and the highest-impact journal in the world. Launched in December 1997 and published monthly. Molecular Cell is dedicated to publishing cutting-edge research in molecular biology, focusing on fundamental cellular processes. The journal encompasses a wide range of topics, including DNA replication, recombination, and repair; Chromatin biology and genome organization; Transcription; RNA processing and decay; Non-coding RNA function; Translation; Protein folding, modification, and quality control; Signal transduction pathways; Cell cycle and checkpoints; Cell death; Autophagy; Metabolism.
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