评估缺血再灌注损伤对大鼠同种异体肌肉和皮肤移植模型的影响。

Fatih Ceran, Salih Onur Basat, İdris Ersin, Deniz Filinte, Özgür Pilancı, Mehmet Bozkurt
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引用次数: 0

摘要

背景:缺血再灌注损伤(IRI)是影响移植存活率的一种现象。我们研究的目的是检测长时间温缺血对同种异体肌肉和皮肤移植模型的影响:方法:用 48 只 Lewis 大鼠和 16 只 Brown-Norway 大鼠分为四组:同种异体腹股沟皮瓣移植组(IST)、同种异体腓肠肌皮瓣移植组(IMT)、同种异体腹股沟皮瓣移植组(AST)和同种异体腓肠肌皮瓣移植组(AMT)。所有组别均在术后第 1、7、21、35、63、100 和 120 天测量丙二醛(MDA)和超氧化物歧化酶(SOD)水平。异体组在术后第 7、21、35、63、100 和 120 天评估了外周血中的捐献者特异性嵌合体(DSC)。术后第 1 天、第 7 天和第 120 天,对所有组的 microRNA-21 和 microRNA-205 水平进行了评估。研究结束时,进行了组织病理学检查:结果:各组间的 MDA 和 SOD 水平差异有统计学意义。AMT 组检测到 DSC。观察到 microRNA-205 明显增加,尤其是在 AMT 组。肌肉移植组之间功能性肌肉单位的数量没有明显差异:结论:值得注意的是,AMT 组存在 DSC,而 IMT 组和 AMT 组的功能性肌肉单位数量无明显差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the effects of ischemia-reperfusion injury in rat isogenic and allogeneic muscle and skin transplant models.

Background: Ischemia-reperfusion injury (IRI) is a phenomenon that affects transplant survival. The aim of our study was to examine the effects of IRI in isogenic and allogeneic muscle and skin transplantation models exposed to prolonged warm ischemia.

Methods: Forty-eight Lewis rats and 16 Brown-Norway rats were used to create four groups: Isogenic Inguinal Flap Transplantation (IST), Isogenic Gastrocnemius Muscle Flap Transplantation (IMT), Allogeneic Inguinal Flap Transplantation (AST), and Allogeneic Gastrocnemius Muscle Flap Transplantation (AMT). Malonyldialdehyde (MDA) and superoxide dismutase (SOD) levels were measured on postoperative days 1, 7, 21, 35, 63, 100, and 120 in all groups. Donor-specific chimerism (DSC) in peripheral blood was evaluated in the allogeneic groups on postoperative days 7, 21, 35, 63, 100, and 120. The microRNA-21 and microRNA-205 levels were evaluated on postoperative days 1, 7, and 120 in all groups. At the end of the study, a histopathological examination was performed.

Results: A statistically significant difference was found between the groups in terms of MDA and SOD levels. DSC was detected in the AMT group. A significant increase in microRNA-205 was observed, especially in the AMT group. There was no significant difference in the number of functional muscle units between the muscle transplantation groups.

Conclusion: The presence of DSC in the AMT group and the lack of a significant difference in the number of functional muscle units in the IMT and AMT groups are noteworthy findings.

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