{"title":"叶绿体五肽重复蛋白 RCN22 通过 TB1-RCN22-RbcL 模块影响糖分水平,从而调节水稻的分蘖数量。","authors":"Tianyu Mo, Tianhao Wang, Yinglu Sun, Ashmit Kumar, Humphrey Mkumbwa, Jingjing Fang, Jinfeng Zhao, Shoujiang Yuan, Zichao Li, Xueyong Li","doi":"10.1016/j.xplc.2024.101073","DOIUrl":null,"url":null,"abstract":"<p><p>As an important yield component, the rice tiller number controls panicle number and determines grain yield. The regulation of rice tiller number by chloroplast pentatricopeptide repeat (PPR) proteins has not been reported. Here, we report a rice reduced culm number22 (rcn22) mutant which produces few tillers due to suppressed tiller bud elongation. Map-based cloning revealed that RCN22 encodes a chloroplast-localized P-type PPR protein. We found that RCN22 specifically binds to the 5'-UTR of RbcL mRNA (encoding the large subunit of Rubisco) and enhances its stability. The reduced RbcL mRNA abundance in rcn22 led to a lower photosynthetic rate and decreased sugar levels. Consequently, transcript levels of DWARF3 (D3) and TEOSINTE BRANCHED1 (TB1) (encoding negative regulators of tiller bud elongation) increased, whereas protein levels of a positive regulator DWARF53 (D53) decreased. Furthermore, high concentrations of sucrose could rescue the tiller bud growth defect of the rcn22 mutant. On the other hand, TB1 directly binds to the RCN22 promoter and downregulates its expression. The tb1/rcn22 double mutant showed a tillering phenotype similar to rcn22. Our results suggest that the TB1-RCN22-RbcL module plays a vital role in rice tiller bud elongation by affecting sugar levels.</p>","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":null,"pages":null},"PeriodicalIF":9.4000,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The chloroplast pentatricopeptide repeat protein RCN22 regulates tiller number in rice by affecting sugar levels via the TB1-RCN22-RbcL module.\",\"authors\":\"Tianyu Mo, Tianhao Wang, Yinglu Sun, Ashmit Kumar, Humphrey Mkumbwa, Jingjing Fang, Jinfeng Zhao, Shoujiang Yuan, Zichao Li, Xueyong Li\",\"doi\":\"10.1016/j.xplc.2024.101073\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>As an important yield component, the rice tiller number controls panicle number and determines grain yield. The regulation of rice tiller number by chloroplast pentatricopeptide repeat (PPR) proteins has not been reported. Here, we report a rice reduced culm number22 (rcn22) mutant which produces few tillers due to suppressed tiller bud elongation. Map-based cloning revealed that RCN22 encodes a chloroplast-localized P-type PPR protein. We found that RCN22 specifically binds to the 5'-UTR of RbcL mRNA (encoding the large subunit of Rubisco) and enhances its stability. The reduced RbcL mRNA abundance in rcn22 led to a lower photosynthetic rate and decreased sugar levels. Consequently, transcript levels of DWARF3 (D3) and TEOSINTE BRANCHED1 (TB1) (encoding negative regulators of tiller bud elongation) increased, whereas protein levels of a positive regulator DWARF53 (D53) decreased. Furthermore, high concentrations of sucrose could rescue the tiller bud growth defect of the rcn22 mutant. On the other hand, TB1 directly binds to the RCN22 promoter and downregulates its expression. The tb1/rcn22 double mutant showed a tillering phenotype similar to rcn22. Our results suggest that the TB1-RCN22-RbcL module plays a vital role in rice tiller bud elongation by affecting sugar levels.</p>\",\"PeriodicalId\":52373,\"journal\":{\"name\":\"Plant Communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.4000,\"publicationDate\":\"2024-08-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Communications\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.xplc.2024.101073\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Communications","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.xplc.2024.101073","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
The chloroplast pentatricopeptide repeat protein RCN22 regulates tiller number in rice by affecting sugar levels via the TB1-RCN22-RbcL module.
As an important yield component, the rice tiller number controls panicle number and determines grain yield. The regulation of rice tiller number by chloroplast pentatricopeptide repeat (PPR) proteins has not been reported. Here, we report a rice reduced culm number22 (rcn22) mutant which produces few tillers due to suppressed tiller bud elongation. Map-based cloning revealed that RCN22 encodes a chloroplast-localized P-type PPR protein. We found that RCN22 specifically binds to the 5'-UTR of RbcL mRNA (encoding the large subunit of Rubisco) and enhances its stability. The reduced RbcL mRNA abundance in rcn22 led to a lower photosynthetic rate and decreased sugar levels. Consequently, transcript levels of DWARF3 (D3) and TEOSINTE BRANCHED1 (TB1) (encoding negative regulators of tiller bud elongation) increased, whereas protein levels of a positive regulator DWARF53 (D53) decreased. Furthermore, high concentrations of sucrose could rescue the tiller bud growth defect of the rcn22 mutant. On the other hand, TB1 directly binds to the RCN22 promoter and downregulates its expression. The tb1/rcn22 double mutant showed a tillering phenotype similar to rcn22. Our results suggest that the TB1-RCN22-RbcL module plays a vital role in rice tiller bud elongation by affecting sugar levels.
期刊介绍:
Plant Communications is an open access publishing platform that supports the global plant science community. It publishes original research, review articles, technical advances, and research resources in various areas of plant sciences. The scope of topics includes evolution, ecology, physiology, biochemistry, development, reproduction, metabolism, molecular and cellular biology, genetics, genomics, environmental interactions, biotechnology, breeding of higher and lower plants, and their interactions with other organisms. The goal of Plant Communications is to provide a high-quality platform for the dissemination of plant science research.