印度北卡纳塔克邦一家三级医院阴道微生物群与自然流产的横断面比较研究。

Nursing for Women''s Health Pub Date : 2024-10-01 DOI:10.1016/j.nwh.2024.04.006

Babita Belgundkar, Sangeeta Kharde, Suneel Dodamani, Shivani Tendulkar

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引用次数: 0

摘要

目的：比较胎龄不足 20 周的自然流产妇女与同胎龄继续妊娠妇女的阴道微生物群：比较胎龄小于 20 周的自然流产妇女与胎龄相同的继续妊娠妇女的阴道微生物群：设计：横断面比较研究设计：研究在印度北卡纳塔克邦的一家三级医院进行。在这个农村地区，许多妇女缺乏产前保健意识和妇女健康信息。鉴于自然流产率不断上升，研究阴道微生物组对妊娠结局的影响对于提高当地的认识至关重要：200 名妇女，其中 100 名在妊娠 20 周内经历过自然流产，另外 100 名在相同孕龄下保持妊娠：采用阴道拭子检测参与者的阴道微生物群。从阴道样本中提取 DNA，采用 16S 核糖体 RNA 测序法鉴定微生物：阴道培养的病原体检出率为 13.15%（n = 200）。自然流产妇女和继续妊娠妇女的细菌检出率分别为 19.9% 和 7.9%（P < .05）。与继续妊娠的妇女相比，自然流产的妇女有五种常见的细菌：大肠杆菌（48%）、粪肠球菌（28%）、粪肠球菌（12%）、荧光假单胞菌（7%）和雪松假单胞菌（5%）；在持续妊娠的妇女中，最常见的菌种是内氏乳杆菌（58%）、脆片乳杆菌（32%）和大肠杆菌（10%；P < .05）：自然流产妇女的阴道微生物组更多样化，细菌分布也更不规则。结论：自然流产妇女阴道微生物组的细菌种类更多，分布更不规则，所发现的细菌可能会影响妇女的妊娠结局，也可能与自然流产有关。护士向处于生育期的妇女提供有关产前检查、性传播感染和会阴护理的教育至关重要。

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A Cross-Sectional Comparative Study of Vaginal Microbiota and Spontaneous Abortion at a Tertiary Care Hospital in North Karnataka, India

Objective

To compare the vaginal microbiome in women who have undergone spontaneous abortion with a gestational age of less than 20 weeks to that of women who have a continued pregnancy of the same gestational age.

Design

Cross-sectional comparative research design.

Setting

The study was conducted in a tertiary care hospital of North Karnataka, India. In this rural area, many women lack awareness of prenatal care and women’s health information. Given rising rates of spontaneous abortion, studying the impact of the vaginal microbiome on pregnancy outcomes is crucial for raising local awareness.

Participants

Two hundred women; 100 experienced spontaneous abortion within 20 weeks’ gestation, and 100 maintained pregnancy with the same gestational age.

Measures

Vaginal swab was used to detect participants’ vaginal microbiota. DNA was extracted from the vaginal samples; the 16S ribosomal RNA sequencing method was used to identify the organisms present.

Results

A pathogen detection rate of 13.15% (n = 200 women) was found in vaginal cultures. The percentages of bacterial detection in women who had spontaneous abortion and those whose pregnancies continued were 19.9% and 7.9%, respectively (p < .05). Compared to women who sustained their pregnancy, women who experienced spontaneous abortion had five common species: Escherichia coli (48%), Enterococcus faecalis (28%), Enterococcus faecium (12%), Pseudomonas fluorescens (7%), and Pseudomonas cedrina (5%); the most prevalent species among those with sustained pregnancy were Lactobacillus iners (58%), Lactobacillus crispatus (32 %), and E. coli (10%; p < .05).

Conclusion

The vaginal microbiomes of women with spontaneous abortion were more varied and irregularly distributed with bacteria. The bacteria identified could affect a woman’s pregnancy outcome and perhaps be associated with spontaneous abortion. It is vital for nurses to provide education to women who are in the fertile period regarding prenatal screening, sexually transmitted infections, and perineal care.

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来源期刊

Nursing for Women''s Health Nursing-Nursing (all)

CiteScore

2.10

自引率

0.00%

发文量

期刊介绍： Nursing for Women"s Health publishes the most recent and compelling health care information on women"s health, newborn care and professional nursing issues. As a refereed, clinical practice journal, it provides professionals involved in providing optimum nursing care for women and their newborns with health care trends and everyday issues in a concise, practical, and easy-to-read format.