传染性支气管炎病毒疫苗接种(而非 XCR1 的存在)与鸡盲肠微生物群的巨大差异有关。

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
Laura Glendinning, Zhiguang Wu, Lonneke Vervelde, Mick Watson, Adam Balic
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引用次数: 0

摘要

鸡的免疫系统和微生物群在维持肠道平衡和抵御病原体方面发挥着至关重要的作用。在哺乳动物中,XCR1+常规树突状细胞(cDCs)位于肠道引流淋巴结,在肠道稳态中发挥着重要作用。这些树突状细胞对肠道腔内容物中的抗原进行采样,并通过产生适当的调节性和效应性 T 细胞反应来限制对肠道共生微生物的炎症反应。我们假设这些细胞在维持鸡的肠道平衡中发挥着类似的作用,而缺乏 XCR1 的鸡很可能含有菌群失调的盲肠微生物群。在这里,我们比较了杂合或同源 XCR1 基因敲除鸡、接种或未接种传染性支气管炎病毒(IBV)疫苗鸡的盲肠微生物群。我们使用短线程(Illumina)和长线程(PacBio HiFi)元基因组测序技术重建了 670 个高质量的菌株级元基因组。我们发现不同基因型之间的阿尔法多样性或特定微生物类群的丰度没有明显差异。然而,我们发现 IBV 疫苗接种与微生物群的丰富度和β多样性以及 40 个细菌属的丰度之间存在显著差异。总之,我们发现缺乏 XCR1 与鸡微生物群的显著变化无关,但接种 IBV 疫苗却与之相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Infectious bronchitis virus vaccination, but not the presence of XCR1, is correlated with large differences in chicken caecal microbiota.

The chicken immune system and microbiota play vital roles in maintaining gut homeostasis and protecting against pathogens. In mammals, XCR1+ conventional dendritic cells (cDCs) are located in the gut-draining lymph nodes and play a major role in gut homeostasis. These cDCs sample antigens in the gut luminal contents and limit the inflammatory response to gut commensal microbes by generating appropriate regulatory and effector T-cell responses. We hypothesized that these cells play similar roles in sustaining gut homeostasis in chickens, and that chickens lacking XCR1 were likely to contain a dysbiotic caecal microbiota. Here we compare the caecal microbiota of chickens that were either heterozygous or homozygous XCR1 knockouts, that had or had not been vaccinated for infectious bronchitis virus (IBV). We used short-read (Illumina) and long-read (PacBio HiFi) metagenomic sequencing to reconstruct 670 high-quality, strain-level metagenome assembled genomes. We found no significant differences between alpha diversity or the abundance of specific microbial taxa between genotypes. However, IBV vaccination was found to correlate with significant differences in the richness and beta diversity of the microbiota, and to the abundance of 40 bacterial genera. In conclusion, we found that a lack of XCR1 was not correlated with significant changes in the chicken microbiota, but IBV vaccination was.

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来源期刊
Microbial Genomics
Microbial Genomics Medicine-Epidemiology
CiteScore
6.60
自引率
2.60%
发文量
153
审稿时长
12 weeks
期刊介绍: Microbial Genomics (MGen) is a fully open access, mandatory open data and peer-reviewed journal publishing high-profile original research on archaea, bacteria, microbial eukaryotes and viruses.
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