巴戟天多糖通过调节 miR-214-3p/NEDD4L 抑制绝经后骨质疏松症小鼠的破骨细胞分化

IF 3.3 3区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Calcified Tissue International Pub Date : 2024-11-01 Epub Date: 2024-08-28 DOI:10.1007/s00223-024-01271-8
Hui Huang, Jian Chen, Xiaomei Lin, Zhengkun Lin
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引用次数: 0

摘要

研究巴戟天多糖(MOPs)通过miR-214-3p及其靶蛋白调节破骨细胞分化和凋亡的潜在机制。对8周龄的雌性C57BL6小鼠进行卵巢切除术,建立绝经后骨质疏松症(PMOP)模型。小鼠立即接受每公斤 500 毫克的澳门巴黎人娱乐官网治疗(预防组);其他小鼠在手术后 2 周接受澳门巴黎人娱乐官网治疗(治疗组)。检测左股骨骨矿密度(BMD)。给 RAW264.7 细胞注射 NF-κB 配体受体激活剂(RANKL)以建立破骨细胞(OC)模型,并用含 1 或 2 g/kg MOPs 的血清进行处理。通过 Western 印迹、定量实时逆转录聚合酶链反应(qRT-PCR)和流式细胞术检测凋亡相关指标、miR-214-3p 和表达发育下调 4-Like(NEDD4L)。OC接受miR-214-3p抑制剂或NEDD4L小干扰RNA(siRNA)。澳门巴黎人娱乐官网逆转了PMOP诱导的骨骼变化。与 RANKL 组相比,MOPs 增加了 OCs 的凋亡和相关标记物。MOPs降低了PMOP小鼠股骨的miR-214-3p(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Morinda Officinalis Polysaccharides Inhibit Osteoclast Differentiation by Regulating miR-214-3p/NEDD4L in Postmenopausal Osteoporosis Mice.

Morinda Officinalis Polysaccharides Inhibit Osteoclast Differentiation by Regulating miR-214-3p/NEDD4L in Postmenopausal Osteoporosis Mice.

To investigate the potential mechanism of Morinda officinalis F. C. How polysaccharides (MOPs) in regulating osteoclast differentiation and apoptosis through miR-214-3p and its target protein. Ovariectomy was performed in 8-week female C57BL6 mice to establish the postmenopausal osteoporosis (PMOP) model. Mice were treated immediately with 500 mg/kg of MOPs (prevention group); others were treated 2 weeks after operation (treatment group). Left femur bone mineral density (BMD) was examined. RAW264.7 cells were administered with receptor activator of NF-κB ligand (RANKL) to establish the osteoclast (OC) model and treated with serum containing 1 or 2 g/kg of MOPs. Apoptosis-related indexes, miR-214-3p, and Expressed Developmentally Down-regulated 4-Like (NEDD4L) were detected by western blot, quantitative real-time-reverse transcription polymerase chain reaction (qRT-PCR), and flow cytometry. OC received a miR-214-3p inhibitor or NEDD4L small interfering RNA (siRNA). MOPs reversed the PMOP-induced changes in bones. Compared with the RANKL group, MOPs increased the apoptosis and related markers in OCs. MOPs decreased the femur miR-214-3p of PMOP mice (P < 0.001). Higher concentrations of MOPs reversed the upregulation of miR-214 mRNA in OCs (P < 0.001). miR-214-3p inhibitor increased the expression of Bax and CC3 (P < 0.01) and decreased the expression of Bcl-2 (P < 0.05). NEDD4L is targeted by miR-214. NEDD4L was upregulated in the RANKL + MOPs group (P < 0.01). miR-214-3p inhibitor increased the upregulation of NEDD4L induced by MOPs (P < 0.05). siRNA NEDD4L significantly reversed the inhibition of MOPs on osteoclast differentiation with miR-214-3p inhibitor (P < 0.01). MOPs effectively prevent PMOP by inhibiting osteoclastogenesis and inducing OC apoptosis through the miR-214-3p/NEDD4L pathway.

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来源期刊
Calcified Tissue International
Calcified Tissue International 医学-内分泌学与代谢
CiteScore
8.00
自引率
2.40%
发文量
112
审稿时长
4-8 weeks
期刊介绍: Calcified Tissue International and Musculoskeletal Research publishes original research and reviews concerning the structure and function of bone, and other musculoskeletal tissues in living organisms and clinical studies of musculoskeletal disease. It includes studies of cell biology, molecular biology, intracellular signalling, and physiology, as well as research into the hormones, cytokines and other mediators that influence the musculoskeletal system. The journal also publishes clinical studies of relevance to bone disease, mineral metabolism, muscle function, and musculoskeletal interactions.
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