Anait S Khalatyan, Anastasiya N Shishparenok, Konstantin S Avetisov, Yulia A Gladilina, Varvara G Blinova, Dmitry D Zhdanov
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引用次数: 0
摘要
背景:年龄在老年性黄斑变性(AMD)的发展过程中起着主要作用。端粒长度(TL)是与衰老最相关的生物标志物之一。在我们的研究中,我们旨在确定TL与T淋巴细胞、B淋巴细胞、NK细胞或单核细胞与不同形式AMD的关系:我们的研究包括 62 名 AMD 患者:地理性萎缩 (GA)、伴有或不伴有黄斑萎缩的新生血管性 AMD (NVAMD),以及 22 名健康对照者。通过免疫磁分离法从外周血中分离出每种白细胞亚型,并纯化其 DNA。基因组 DNA 中的 TL 采用 qPCR 方法测定,方法是用特异性寡核苷酸引物扩增端粒区,并与对照基因进行归一化。统计分析使用 R 4.5.1 版进行:我们观察到对照组和 NVAMD 组的 T 细胞端粒长度有明显的统计学增长,而 GA 组则没有。在所有 AMD 组中,B 细胞和单核细胞的 TL 均显著下降。结论:单核细胞的TL与AMD的关系最为密切。它反映了一个人的 "端粒状态",并可能成为这些退化过程的诊断标志。
Association of Telomere Length in T Lymphocytes, B Lymphocytes, NK Cells and Monocytes with Different Forms of Age-Related Macular Degeneration.
Background: Age plays a primary role in the development of age-related macular degeneration (AMD). Telomere length (TL) is one of the most relevant biomarkers of aging. In our study, we aimed to determine the association of TL with T lymphocytes, B lymphocytes, NK cells or monocytes with different forms of AMD.
Methods: Our study included 62 patients with AMD: geographic atrophy (GA), neovascular AMD (NVAMD) with and without macular atrophy and 22 healthy controls. Each leukocyte subtype was isolated from peripheral blood by immunomagnetic separation, and the DNA was purified. The TL in the genomic DNA was determined using qPCR by amplifying the telomere region with specific oligonucleotide primers and normalizing to the control gene. Statistical analysis was performed using R version 4.5.1.
Results: We observed a statistically significant increase in TL in the T cells between the control and NVAMD groups but not for the GA group. The B cells and monocytes showed a significant decrease in TL in all AMD groups. The TL in the NK cells did not decrease in any of the AMD groups.
Conclusions: The TL in the monocytes had the strongest association with AMD. It reflects a person's "telomeric status" and may become a diagnostic hallmark of these degenerative processes.
BiomedicinesBiochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology
CiteScore
5.20
自引率
8.50%
发文量
2823
审稿时长
8 weeks
期刊介绍:
Biomedicines (ISSN 2227-9059; CODEN: BIOMID) is an international, scientific, open access journal on biomedicines published quarterly online by MDPI.