Leshaia Davis-Johnson BS, Rebecca Lightman BS, Conner Lynn BS, Dawn Caster BS, David Powell PhD, Nicholas Short PhD
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Logistic regression was used to generate a receiver operating characteristic (ROC) curve, which provided an optimal cutoff concentration. Comparisons of all groups were analyzed using a Kruskal-Wallis test with post-hoc corrected Dunn's tests. Paired LN patient samples (active LN dates to inactive LN) were compared using a two-tailed Wilcoxon matched pairs signed rank test with Bonferroni correction for multiple comparisons.</p></div><div><h3>Results</h3><p>A-1AG are significantly more abundant in urine of Active proliferative LN patients’ more α-1AG than HD, patients with membranous LN, MCD, or MGN. An optimal cutoff concentration was determined using ROC curves at 89,230.77 ng/mL.</p></div><div><h3>Conclusion</h3><p>⍺-1AG appears to be a promising diagnostic marker for proliferative LN. Moreover, it also appears to be a marker for disease activity in proliferative LN. Neutrophils may release some of the ⍺-1AG that appears in LN patients’ urine. The role of neutrophil-derived ⍺-1AG on renal injury remains to be determined.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Page 418"},"PeriodicalIF":2.5000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"α-1AG is a Useful Urinary Biomarker In Proliferative Lupus Nephritis\",\"authors\":\"Leshaia Davis-Johnson BS, Rebecca Lightman BS, Conner Lynn BS, Dawn Caster BS, David Powell PhD, Nicholas Short PhD\",\"doi\":\"10.1016/j.jnma.2024.07.018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>Lupus nephritis (LN) is kidney inflammation and injury that commonly results from systemic lupus erythematosus (SLE). Neutrophils contribute to LN is by releasing contents from preformed granules that damage the kidney. Neutrophils release α-1 acid glycoprotein (α-1AG) in secondary and tertiary granules. The purpose of this study was to investigate α-1AG as a candidate biomarker in proliferative lupus nephritis.</p></div><div><h3>Methods</h3><p>ELISAs were used to measure α-1AG in urine from patients with proliferative lupus nephritis, membranous lupus nephritis, minimal change disease (MCD), membranous glomerulonephritis (MGN), and healthy donor (HD) controls. α-1AG was also measured in neutrophil supernatants. Logistic regression was used to generate a receiver operating characteristic (ROC) curve, which provided an optimal cutoff concentration. Comparisons of all groups were analyzed using a Kruskal-Wallis test with post-hoc corrected Dunn's tests. Paired LN patient samples (active LN dates to inactive LN) were compared using a two-tailed Wilcoxon matched pairs signed rank test with Bonferroni correction for multiple comparisons.</p></div><div><h3>Results</h3><p>A-1AG are significantly more abundant in urine of Active proliferative LN patients’ more α-1AG than HD, patients with membranous LN, MCD, or MGN. An optimal cutoff concentration was determined using ROC curves at 89,230.77 ng/mL.</p></div><div><h3>Conclusion</h3><p>⍺-1AG appears to be a promising diagnostic marker for proliferative LN. Moreover, it also appears to be a marker for disease activity in proliferative LN. Neutrophils may release some of the ⍺-1AG that appears in LN patients’ urine. The role of neutrophil-derived ⍺-1AG on renal injury remains to be determined.</p></div>\",\"PeriodicalId\":17369,\"journal\":{\"name\":\"Journal of the National Medical Association\",\"volume\":\"116 4\",\"pages\":\"Page 418\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the National Medical Association\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0027968424000993\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the National Medical Association","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0027968424000993","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
α-1AG is a Useful Urinary Biomarker In Proliferative Lupus Nephritis
Purpose
Lupus nephritis (LN) is kidney inflammation and injury that commonly results from systemic lupus erythematosus (SLE). Neutrophils contribute to LN is by releasing contents from preformed granules that damage the kidney. Neutrophils release α-1 acid glycoprotein (α-1AG) in secondary and tertiary granules. The purpose of this study was to investigate α-1AG as a candidate biomarker in proliferative lupus nephritis.
Methods
ELISAs were used to measure α-1AG in urine from patients with proliferative lupus nephritis, membranous lupus nephritis, minimal change disease (MCD), membranous glomerulonephritis (MGN), and healthy donor (HD) controls. α-1AG was also measured in neutrophil supernatants. Logistic regression was used to generate a receiver operating characteristic (ROC) curve, which provided an optimal cutoff concentration. Comparisons of all groups were analyzed using a Kruskal-Wallis test with post-hoc corrected Dunn's tests. Paired LN patient samples (active LN dates to inactive LN) were compared using a two-tailed Wilcoxon matched pairs signed rank test with Bonferroni correction for multiple comparisons.
Results
A-1AG are significantly more abundant in urine of Active proliferative LN patients’ more α-1AG than HD, patients with membranous LN, MCD, or MGN. An optimal cutoff concentration was determined using ROC curves at 89,230.77 ng/mL.
Conclusion
⍺-1AG appears to be a promising diagnostic marker for proliferative LN. Moreover, it also appears to be a marker for disease activity in proliferative LN. Neutrophils may release some of the ⍺-1AG that appears in LN patients’ urine. The role of neutrophil-derived ⍺-1AG on renal injury remains to be determined.
期刊介绍：
Journal of the National Medical Association, the official journal of the National Medical Association, is a peer-reviewed publication whose purpose is to address medical care disparities of persons of African descent.
The Journal of the National Medical Association is focused on specialized clinical research activities related to the health problems of African Americans and other minority groups. Special emphasis is placed on the application of medical science to improve the healthcare of underserved populations both in the United States and abroad. The Journal has the following objectives: (1) to expand the base of original peer-reviewed literature and the quality of that research on the topic of minority health; (2) to provide greater dissemination of this research; (3) to offer appropriate and timely recognition of the significant contributions of physicians who serve these populations; and (4) to promote engagement by member and non-member physicians in the overall goals and objectives of the National Medical Association.
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