转录组分析有助于深入了解受到副溶血性弧菌挑战的蝲蛄肝胰腺中的免疫反应基因。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Jun-Jie Jiang , Dan-Dan Bian , Xin Liu , Dai-Zhen Zhang , Qiu-Ning Liu , Bo-Ping Tang , Mei-Ling Zhang
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引用次数: 0

摘要

克氏原螯虾是中国的重要经济鱼类,但其因病原菌(尤其是副溶血性弧菌)引起的高死亡率造成了巨大的经济损失。本研究旨在通过比较和分析小龙虾肝胰腺组织的转录组数据,了解小龙虾对细菌感染的免疫反应。分析了生理指标(TP、Alb、ACP 和 AKP),并制备了组织切片。在对数据进行组合和注释后,确定了 18,756 个单基因。通过比较对照组和副溶血性弧菌组中这些单基因的表达水平,发现了 4037 个 DEGs,其中 2278 个单基因在副溶血性弧菌组中上调,1759 个下调。GO(基因本体)富集分析表明,DGEs主要富集在细胞解剖活动、结合和细胞过程中;KEGG(京都基因组百科全书)通路富集分析表明,DGEs主要富集在碱基切除修复、吞噬体和长寿调节通路中。同时,溶酶体也被富集。吞噬体和溶酶体通路在小龙虾对副溶血性弧菌注射的免疫防御中发挥着重要作用,这一点将得到强调。此外,还利用 qRT-PCR 技术测定了六个选定的免疫相关 DEGs 的表达水平,从而验证了 RNA-seq 分析的结果。这项研究为研究克氏原螯虾的免疫系统和防御机制提供了一个新的视角,为进一步研究该物种的分子免疫机制奠定了宝贵的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transcriptomic analysis provides insights into the immune responsive genes in the Procambarus clarkii hepatopancreas challenged with Vibrio parahaemolyticus

Transcriptomic analysis provides insights into the immune responsive genes in the Procambarus clarkii hepatopancreas challenged with Vibrio parahaemolyticus

Procambarus clarkii is an economically important species in China; however, its high mortality rate due to pathogenic bacteria, particularly Vibrio parahaemolyticus, results in significant economic loss. This study aimed to understand the immune response of crayfish to bacterial infection by comparing and analyzing transcriptome data of hepatopancreatic tissue from P. clarkii challenged with V. parahaemolyticus or treated with PBS. Physiological indices (TP, Alb, ACP, and AKP) were analyzed, and tissue sections were prepared. After assembling and annotating the data, 18,756 unigenes were identified. A comparison of the expression levels of these unigenes between the control and V. parahaemolyticus groups revealed 4037 DEGs, with 2278 unigenes upregulated and 1759 downregulated in the V. parahaemolyticus group. GO (Gene Ontology) enrichment analysis shows that the DGEs are mainly enriched in cellular anatomical activity, bindinga and cellular process, enrichment analysis of KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways showed that DGEs were mainly enriched in Base excision repair, Phagosome and Longevity regulating pathway. At the same time, lysosome was also enriched. The phagosome and lysosome pathways play a crucial role in the immune defense of crayfish against V. parahaemolyticus injection that will be highlighted. In addition, the expression levels of six selected immune-related DEGs were measured using qRT-PCR, which validated the results of RNA-seq analysis. This study provides a new perspective on the immune system and defense mechanisms of P. clarkii and a valuable foundation for further investigation of the molecular immune mechanisms of this species.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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