{"title":"口服胡椒碱可改善实验性自身免疫性葡萄膜炎","authors":"Alireza Ghavami, Seyyed Meysam Abtahi Froushani, Aliasghar Tehrani","doi":"10.1007/s10753-024-02131-1","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to evaluate the impact of piperine on experimental autoimmune uveitis (EAU). EAU was induced by immunization with interphotoreceptor retinoid-binding protein emulsified in complete Freund adjuvant. Starting from day 8 post-induction, Lewis rats were given piperine (0, 20, 40, and 80 mg/kg-P.O.) or prednisolone (10 mg/kg-P.O.) for 18 consecutive days. The 80 mg/kg dose of piperine demonstrated superior regression of clinical symptoms, increased nitric oxide levels, and enhanced IDO activity in eye homogenates compared to other doses. The 40 and 80 mg/kg doses of piperine were more effective in promoting weight gain in EAU rats than the 20 mg/kg dose. EAU rats treated with 80 mg/kg piperine showed more favorable mRNA expression of IL-10 and TGF-β in their eyes than other treatment groups. The interventions led to a significant decrease in mRNA ratios of T-bet/GATA-3, RORγt/T-bet, RORγt/Foxp3, and RORγt/GATA-3 in the eyes of EAU rats compared to untreated EAU rats. Specifically, EAU rats treated with 80 mg/kg piperine exhibited a greater reduction in the mRNA ratio of RORγt/Foxp3 expression compared to other treatment groups. Overall, oral administration of piperine may offer potential for clinical application in uveitis.</p>","PeriodicalId":13524,"journal":{"name":"Inflammation","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Oral Administration of Piperine Ameliorates Experimental Autoimmune Uveitis.\",\"authors\":\"Alireza Ghavami, Seyyed Meysam Abtahi Froushani, Aliasghar Tehrani\",\"doi\":\"10.1007/s10753-024-02131-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to evaluate the impact of piperine on experimental autoimmune uveitis (EAU). EAU was induced by immunization with interphotoreceptor retinoid-binding protein emulsified in complete Freund adjuvant. Starting from day 8 post-induction, Lewis rats were given piperine (0, 20, 40, and 80 mg/kg-P.O.) or prednisolone (10 mg/kg-P.O.) for 18 consecutive days. The 80 mg/kg dose of piperine demonstrated superior regression of clinical symptoms, increased nitric oxide levels, and enhanced IDO activity in eye homogenates compared to other doses. The 40 and 80 mg/kg doses of piperine were more effective in promoting weight gain in EAU rats than the 20 mg/kg dose. EAU rats treated with 80 mg/kg piperine showed more favorable mRNA expression of IL-10 and TGF-β in their eyes than other treatment groups. The interventions led to a significant decrease in mRNA ratios of T-bet/GATA-3, RORγt/T-bet, RORγt/Foxp3, and RORγt/GATA-3 in the eyes of EAU rats compared to untreated EAU rats. Specifically, EAU rats treated with 80 mg/kg piperine exhibited a greater reduction in the mRNA ratio of RORγt/Foxp3 expression compared to other treatment groups. Overall, oral administration of piperine may offer potential for clinical application in uveitis.</p>\",\"PeriodicalId\":13524,\"journal\":{\"name\":\"Inflammation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Inflammation\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10753-024-02131-1\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Inflammation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10753-024-02131-1","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Oral Administration of Piperine Ameliorates Experimental Autoimmune Uveitis.
This study aimed to evaluate the impact of piperine on experimental autoimmune uveitis (EAU). EAU was induced by immunization with interphotoreceptor retinoid-binding protein emulsified in complete Freund adjuvant. Starting from day 8 post-induction, Lewis rats were given piperine (0, 20, 40, and 80 mg/kg-P.O.) or prednisolone (10 mg/kg-P.O.) for 18 consecutive days. The 80 mg/kg dose of piperine demonstrated superior regression of clinical symptoms, increased nitric oxide levels, and enhanced IDO activity in eye homogenates compared to other doses. The 40 and 80 mg/kg doses of piperine were more effective in promoting weight gain in EAU rats than the 20 mg/kg dose. EAU rats treated with 80 mg/kg piperine showed more favorable mRNA expression of IL-10 and TGF-β in their eyes than other treatment groups. The interventions led to a significant decrease in mRNA ratios of T-bet/GATA-3, RORγt/T-bet, RORγt/Foxp3, and RORγt/GATA-3 in the eyes of EAU rats compared to untreated EAU rats. Specifically, EAU rats treated with 80 mg/kg piperine exhibited a greater reduction in the mRNA ratio of RORγt/Foxp3 expression compared to other treatment groups. Overall, oral administration of piperine may offer potential for clinical application in uveitis.
期刊介绍:
Inflammation publishes the latest international advances in experimental and clinical research on the physiology, biochemistry, cell biology, and pharmacology of inflammation. Contributions include full-length scientific reports, short definitive articles, and papers from meetings and symposia proceedings. The journal''s coverage includes acute and chronic inflammation; mediators of inflammation; mechanisms of tissue injury and cytotoxicity; pharmacology of inflammation; and clinical studies of inflammation and its modification.