Saba Sane, Vera Ebrahimi, Zeinab Shirvani Farsani, Soudeh Ghafouri-Fard
{"title":"自闭症患者体内 lncRNAs 的表达评估","authors":"Saba Sane, Vera Ebrahimi, Zeinab Shirvani Farsani, Soudeh Ghafouri-Fard","doi":"10.1007/s12031-024-02258-8","DOIUrl":null,"url":null,"abstract":"<div><p>Autism is a severe neurodevelopmental condition with unknown pathobiology. Nevertheless, multiple pieces of evidence suggest long non-coding RNA (lncRNA) dysregulation may be a contributing factor to this disorder. We investigated the association between the expression of five specific lncRNAs and autism. Peripheral blood was collected from 30 children with autism and 41 healthy children. The expression levels of <i>PCAT-29</i>, <i>lincRNA-ROR</i>, <i>LINC-PINT</i>, <i>lincRNA-p21</i>, and <i>PCAT-1</i> were calculated. Then, their significance as biomarkers was also evaluated. The expression of <i>LincRNA-ROR</i> (27 times), <i>LINC-PINT</i> (5.26 times), <i>LincRNA-p21</i> (4.54 times), <i>PCAT-29</i> (16.66 times), and <i>PCAT-1</i> (25 times) genes was significantly decreased in patients compared to the control group (<i>p</i> values < 0.05). According to the ROC curve analysis for each lncRNA, <i>LincRNA-ROR</i>, <i>LINC-PINT</i>, <i>LincRNA-p21</i>, <i>PCAT-29</i>, and <i>PCAT-1</i> lncRNAs with diagnostic power of 0.85, 0.67, 0.64, 0.74, and 0.84, respectively, could be used as diagnostic biomarkers for autism. Additionally, significant positive correlations were reported between expression levels of <i>PCAT-1</i> and <i>PCAT-29</i> genes. Moreover, a positive correlation was detected between expression levels of <i>lincRNA-ROR</i> and patients’ age. The current study shows further pieces of evidence for deregulation of lncRNAs in autistic patients that show these lncRNAs may play an important part in the pathogenesis of ASD. However, the role of lncRNA in the neurobiology of autism needs to be investigated further.</p></div>","PeriodicalId":652,"journal":{"name":"Journal of Molecular Neuroscience","volume":"74 3","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Assessment of Expression of lncRNAs in Autistic Patients\",\"authors\":\"Saba Sane, Vera Ebrahimi, Zeinab Shirvani Farsani, Soudeh Ghafouri-Fard\",\"doi\":\"10.1007/s12031-024-02258-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Autism is a severe neurodevelopmental condition with unknown pathobiology. Nevertheless, multiple pieces of evidence suggest long non-coding RNA (lncRNA) dysregulation may be a contributing factor to this disorder. We investigated the association between the expression of five specific lncRNAs and autism. Peripheral blood was collected from 30 children with autism and 41 healthy children. The expression levels of <i>PCAT-29</i>, <i>lincRNA-ROR</i>, <i>LINC-PINT</i>, <i>lincRNA-p21</i>, and <i>PCAT-1</i> were calculated. Then, their significance as biomarkers was also evaluated. The expression of <i>LincRNA-ROR</i> (27 times), <i>LINC-PINT</i> (5.26 times), <i>LincRNA-p21</i> (4.54 times), <i>PCAT-29</i> (16.66 times), and <i>PCAT-1</i> (25 times) genes was significantly decreased in patients compared to the control group (<i>p</i> values < 0.05). According to the ROC curve analysis for each lncRNA, <i>LincRNA-ROR</i>, <i>LINC-PINT</i>, <i>LincRNA-p21</i>, <i>PCAT-29</i>, and <i>PCAT-1</i> lncRNAs with diagnostic power of 0.85, 0.67, 0.64, 0.74, and 0.84, respectively, could be used as diagnostic biomarkers for autism. Additionally, significant positive correlations were reported between expression levels of <i>PCAT-1</i> and <i>PCAT-29</i> genes. Moreover, a positive correlation was detected between expression levels of <i>lincRNA-ROR</i> and patients’ age. The current study shows further pieces of evidence for deregulation of lncRNAs in autistic patients that show these lncRNAs may play an important part in the pathogenesis of ASD. However, the role of lncRNA in the neurobiology of autism needs to be investigated further.</p></div>\",\"PeriodicalId\":652,\"journal\":{\"name\":\"Journal of Molecular Neuroscience\",\"volume\":\"74 3\",\"pages\":\"\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Molecular Neuroscience\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s12031-024-02258-8\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Neuroscience","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s12031-024-02258-8","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Assessment of Expression of lncRNAs in Autistic Patients
Autism is a severe neurodevelopmental condition with unknown pathobiology. Nevertheless, multiple pieces of evidence suggest long non-coding RNA (lncRNA) dysregulation may be a contributing factor to this disorder. We investigated the association between the expression of five specific lncRNAs and autism. Peripheral blood was collected from 30 children with autism and 41 healthy children. The expression levels of PCAT-29, lincRNA-ROR, LINC-PINT, lincRNA-p21, and PCAT-1 were calculated. Then, their significance as biomarkers was also evaluated. The expression of LincRNA-ROR (27 times), LINC-PINT (5.26 times), LincRNA-p21 (4.54 times), PCAT-29 (16.66 times), and PCAT-1 (25 times) genes was significantly decreased in patients compared to the control group (p values < 0.05). According to the ROC curve analysis for each lncRNA, LincRNA-ROR, LINC-PINT, LincRNA-p21, PCAT-29, and PCAT-1 lncRNAs with diagnostic power of 0.85, 0.67, 0.64, 0.74, and 0.84, respectively, could be used as diagnostic biomarkers for autism. Additionally, significant positive correlations were reported between expression levels of PCAT-1 and PCAT-29 genes. Moreover, a positive correlation was detected between expression levels of lincRNA-ROR and patients’ age. The current study shows further pieces of evidence for deregulation of lncRNAs in autistic patients that show these lncRNAs may play an important part in the pathogenesis of ASD. However, the role of lncRNA in the neurobiology of autism needs to be investigated further.
期刊介绍：
The Journal of Molecular Neuroscience is committed to the rapid publication of original findings that increase our understanding of the molecular structure, function, and development of the nervous system. The criteria for acceptance of manuscripts will be scientific excellence, originality, and relevance to the field of molecular neuroscience. Manuscripts with clinical relevance are especially encouraged since the journal seeks to provide a means for accelerating the progression of basic research findings toward clinical utilization. All experiments described in the Journal of Molecular Neuroscience that involve the use of animal or human subjects must have been approved by the appropriate institutional review committee and conform to accepted ethical standards.
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