{"title":"转录组分析揭示了醋酸法尼酯处理 Metisa plana 后的代谢解毒和免疫反应机制","authors":"","doi":"10.1016/j.compbiolchem.2024.108176","DOIUrl":null,"url":null,"abstract":"<div><p><em>Metisa plana</em> is a widespread insect pest infesting oil palm plantations in Malaysia. Farnesyl acetate (FA), a juvenile hormone analogue, has been reported to exert <em>in vitro</em> and <em>in vivo</em> insecticidal activity against other insect pests. However, the insecticidal mechanism of FA on <em>M. plana</em> remains unclear. Therefore, this study aims to elucidate responsive genes in <em>M. plana</em> in response to FA treatment. The RNA-sequencing reads of FA-treated <em>M. plana</em> were <em>de novo</em>-assembled with existing raw reads from non-treated third instar larvae, and 55,807 transcripts were functionally annotated to multiple protein databases. Several insecticide detoxification-related genes were differentially regulated among the 321 differentially expressed transcripts. Cytochrome P450 monooxygenase, carboxylesterase, and ATP-binding cassette protein were upregulated, while peptidoglycan recognition protein was downregulated. Innate immune response genes, such as glutathione S-transferases, acetylcholinesterase, and heat shock protein, were also identified in the transcriptome. The findings signify that changes occurred in the insect’s receptor and signaling, metabolic detoxification of insecticides, and immune responses upon FA treatment on <em>M. plana</em>. This valuable information on FA toxicity may be used to formulate more effective biorational insecticides for better <em>M. plana</em> pest management strategies in oil palm plantations.</p></div>","PeriodicalId":10616,"journal":{"name":"Computational Biology and Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transcriptome analysis reveals mechanisms of metabolic detoxification and immune responses following farnesyl acetate treatment in Metisa plana\",\"authors\":\"\",\"doi\":\"10.1016/j.compbiolchem.2024.108176\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Metisa plana</em> is a widespread insect pest infesting oil palm plantations in Malaysia. Farnesyl acetate (FA), a juvenile hormone analogue, has been reported to exert <em>in vitro</em> and <em>in vivo</em> insecticidal activity against other insect pests. However, the insecticidal mechanism of FA on <em>M. plana</em> remains unclear. Therefore, this study aims to elucidate responsive genes in <em>M. plana</em> in response to FA treatment. The RNA-sequencing reads of FA-treated <em>M. plana</em> were <em>de novo</em>-assembled with existing raw reads from non-treated third instar larvae, and 55,807 transcripts were functionally annotated to multiple protein databases. Several insecticide detoxification-related genes were differentially regulated among the 321 differentially expressed transcripts. Cytochrome P450 monooxygenase, carboxylesterase, and ATP-binding cassette protein were upregulated, while peptidoglycan recognition protein was downregulated. Innate immune response genes, such as glutathione S-transferases, acetylcholinesterase, and heat shock protein, were also identified in the transcriptome. The findings signify that changes occurred in the insect’s receptor and signaling, metabolic detoxification of insecticides, and immune responses upon FA treatment on <em>M. plana</em>. This valuable information on FA toxicity may be used to formulate more effective biorational insecticides for better <em>M. plana</em> pest management strategies in oil palm plantations.</p></div>\",\"PeriodicalId\":10616,\"journal\":{\"name\":\"Computational Biology and Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Computational Biology and Chemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1476927124001646\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Computational Biology and Chemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1476927124001646","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
Metisa plana 是马来西亚油棕种植园中广泛存在的一种害虫。据报道,乙酸法呢酯(FA)是一种幼虫激素类似物,对其他害虫具有体外和体内杀虫活性。然而,FA 对 M. plana 的杀虫机制仍不清楚。因此,本研究旨在阐明 M. plana 对 FA 处理的响应基因。用未经处理的第三龄幼虫的现有原始读数重新组装了经 FA 处理的 M. plana 的 RNA 序列读数,并将 55 807 个转录本与多个蛋白质数据库进行了功能注释。在 321 个差异表达的转录本中,有几个与杀虫剂解毒相关的基因受到了差异调控。细胞色素 P450 单氧化酶、羧酸酯酶和 ATP 结合盒蛋白被上调,而肽聚糖识别蛋白被下调。转录组中还发现了谷胱甘肽 S-转移酶、乙酰胆碱酯酶和热休克蛋白等先天免疫反应基因。这些研究结果表明,在对 M. plana 进行 FA 处理后,昆虫的受体和信号传导、杀虫剂的代谢解毒以及免疫反应都发生了变化。这些有关 FA 毒性的宝贵信息可用于配制更有效的生物杀虫剂,以改进油棕种植园中的扁叶金龟子害虫管理策略。
Transcriptome analysis reveals mechanisms of metabolic detoxification and immune responses following farnesyl acetate treatment in Metisa plana
Metisa plana is a widespread insect pest infesting oil palm plantations in Malaysia. Farnesyl acetate (FA), a juvenile hormone analogue, has been reported to exert in vitro and in vivo insecticidal activity against other insect pests. However, the insecticidal mechanism of FA on M. plana remains unclear. Therefore, this study aims to elucidate responsive genes in M. plana in response to FA treatment. The RNA-sequencing reads of FA-treated M. plana were de novo-assembled with existing raw reads from non-treated third instar larvae, and 55,807 transcripts were functionally annotated to multiple protein databases. Several insecticide detoxification-related genes were differentially regulated among the 321 differentially expressed transcripts. Cytochrome P450 monooxygenase, carboxylesterase, and ATP-binding cassette protein were upregulated, while peptidoglycan recognition protein was downregulated. Innate immune response genes, such as glutathione S-transferases, acetylcholinesterase, and heat shock protein, were also identified in the transcriptome. The findings signify that changes occurred in the insect’s receptor and signaling, metabolic detoxification of insecticides, and immune responses upon FA treatment on M. plana. This valuable information on FA toxicity may be used to formulate more effective biorational insecticides for better M. plana pest management strategies in oil palm plantations.
期刊介绍:
Computational Biology and Chemistry publishes original research papers and review articles in all areas of computational life sciences. High quality research contributions with a major computational component in the areas of nucleic acid and protein sequence research, molecular evolution, molecular genetics (functional genomics and proteomics), theory and practice of either biology-specific or chemical-biology-specific modeling, and structural biology of nucleic acids and proteins are particularly welcome. Exceptionally high quality research work in bioinformatics, systems biology, ecology, computational pharmacology, metabolism, biomedical engineering, epidemiology, and statistical genetics will also be considered.
Given their inherent uncertainty, protein modeling and molecular docking studies should be thoroughly validated. In the absence of experimental results for validation, the use of molecular dynamics simulations along with detailed free energy calculations, for example, should be used as complementary techniques to support the major conclusions. Submissions of premature modeling exercises without additional biological insights will not be considered.
Review articles will generally be commissioned by the editors and should not be submitted to the journal without explicit invitation. However prospective authors are welcome to send a brief (one to three pages) synopsis, which will be evaluated by the editors.