优化木薯(Manihot esculenta Crantz)嫁接技术,促进其在木薯种植中的应用

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES
MethodsX Pub Date : 2024-08-19 DOI:10.1016/j.mex.2024.102904
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引用次数: 0

摘要

嫁接技术已被成功应用于提高对生物和非生物胁迫的抗性、增加产量、提高果实质量和研究植物的系统信号传递。这项技术尚未在木薯上得到充分应用,目前还没有针对该物种的标准化嫁接方法,尤其是在非洲。这是第一份关于非洲木薯嫁接规程的报告,该规程具有宝贵的优势,包括利用成本效益高且环保的木质愈合室。在这项研究中,我们描述了利用木质愈合室进行木薯裂隙嫁接的优化方案,并概述了产生高嫁接成功率的最佳条件下的分步程序。利用具有高重现性和高成功率的顶楔嫁接技术,我们为木薯(M. esculenta)栽培品种开发出了一种简单、稳健的嫁接方案。对嫁接成功率进行了记录,该方案的嫁接成功率高达 90%,其可重复性使其适用于大规模生产,从而满足了高效木薯繁殖的需求。这种嫁接方法对专业设备和专业知识的要求较低,因此更容易为资源有限的农民和研究人员所接受,从而促进了嫁接在木薯生长、产量提高和植物系统性远距离信号传递等高级研究中的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Optimization of cassava (Manihot esculenta Crantz) grafting technique to enhance its adoption in cassava cultivation

Optimization of cassava (Manihot esculenta Crantz) grafting technique to enhance its adoption in cassava cultivation

Grafting techniques have been successfully adopted to improve resistance to biotic and abiotic stresses, increase yields, fruit quality and study systemic signaling in plants. This technique has not been fully explored in cassava and there is currently no standardized grafting method for this species published especially in Africa. This is the first report on cassava grafting protocol in Africa with valuable advantages including utilizing a cost-effective and environmentally friendly wooden healing chamber. In this study, we describe an optimized cleft grafting protocol for cassava utilizing a wooden healing chamber and outline the step-by-step procedure with optimum conditions to generate a high grafting success rate. Using a top wedge grafting technique with high reproducibility and success rates, we developed a straightforward and robust grafting protocol for cassava (M. esculenta) cultivars. Grafting success was recorded and this protocol produced a high grafting success of 90 % and its reproducibility makes it suitable for mass production thereby addressing the need for efficient cassava propagation. This grafting protocol requires less specialized equipment and expertise making it more accessible to farmers and researchers with limited resources to promote the use of grafting for cassava growth, yield improvement and advanced studies such as systemic long-distance signaling in plants.

  • Optimization of cleft grafting method obtains a high success grafting rate of cassava.

  • A wooden healing chamber provides a controlled environment for graft healing.

  • Promoting cassava grafting; a priority to produce new cultivars and explore breeding research prospects.

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来源期刊
MethodsX
MethodsX Health Professions-Medical Laboratory Technology
CiteScore
3.60
自引率
5.30%
发文量
314
审稿时长
7 weeks
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