整合全息技术和独立于培养的系统可提高对持续性念珠菌血症的检测:一项观察性研究的数据。

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Anna Maria Peri, Kevin O'Callaghan, Nastaran Rafiei, Haakon Bergh, Alexis Tabah, Mark D Chatfield, Patrick Na Harris, David L Paterson
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引用次数: 0

摘要

简介血液培养对念珠菌血症的敏感性较低。不依赖培养的 T2 磁共振(T2)系统可提高灵敏度。SeptiCyte RAPID 是一种宿主反应检测方法,通过评分系统(SeptiScore)量化感染相关炎症的风险。我们研究了 SeptiScore 在检测传统培养和 T2 定义的持续性念珠菌血症方面的性能:这是一项针对念珠菌血症患者的前瞻性多中心观察研究。在指标培养后连续 4 天采集血培养物和用于 T2 和 SeptiCyte 评估的血样。研究人员探讨了 SeptiScore 预测持续性念珠菌血症的性能,持续性念珠菌血症的定义是:(1)随访血培养阳性;(2)随访血培养阳性或 T2 样本阳性:结果:10 名患者入选,包括用 3 种方法评估的 34 份采血样本。总体而言,4/34(12%)的随访血培养结果呈阳性,6/34(18%)的 T2 样本呈阳性。混合模型显示,如果将持续性念珠菌血症定义为随访血培养或T2样本阳性(0.82,95%CI 0.06-1.58),则SeptiScores得分明显较高,但如果仅定义为随访血培养阳性(-0.57,95%CI -1.28-0.14 ),则与持续性念珠菌血症无关。通过SeptiScore检测第1天随访时的持续性念珠菌血症的ROC曲线显示,当念珠菌血症由随访血培养阳性定义时,AUC为0.85(95%CI为0.52-1.00);当念珠菌血症由两种方法定义时,AUC为1.00(95%CI为1.00-1.00):结论:将转录组图谱分析与独立于培养的系统和传统培养相结合,可提高我们诊断顽固性念珠菌血症的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Integrating omics techniques and culture-independent systems may improve the detection of persistent candidemia: data from an observational study.

Introduction: Blood cultures have low sensitivity for candidemia. Sensitivity can be improved by the culture-independent system T2 Magnetic Resonance (T2). SeptiCyte RAPID is a host response assay quantifying the risk of infection-related inflammation through a scoring system (SeptiScore). We investigate the performance of SeptiScore in detecting persistent candidemia as defined by conventional cultures and T2.

Methods: This is a prospective multicentre observational study on patients with candidemia. Blood cultures and blood samples for assessment by T2 and SeptiCyte were collected for 4 consecutive days after the index culture. The performance of SeptiScore was explored to predict persistent candidemia as defined by (1) positive follow-up blood culture (2) either positive follow-up blood culture or T2 sample.

Results: 10 patients were enrolled including 34 blood collections assessed with the 3 methods. Overall, 4/34 (12%) follow-up blood cultures and 6/34 (18%) T2 samples were positive. A mixed model showed significantly higher SeptiScores associated with persistent candidemia when this was defined as either a positive follow-up blood culture or T2 sample (0.82, 95%CI 0.06 to 1.58) but not when this was defined as a positive follow-up blood culture only (-0.57, 95%CI -1.28 to 0.14). ROC curve for detection of persistent candidemia by SeptiScore at day 1 follow-up showed an AUC of 0.85 (95%CI 0.52-1.00) when candidemia was defined by positive follow-up blood culture, and an AUC of 1.00 (95%CI 1.00-1.00) when candidemia was defined according to both methods.

Conclusion: Integrating transcriptome profiling with culture-independent systems and conventional cultures may increase our ability to diagnose persistent candidemia.

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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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