低表达 Lnc-ENST00000535078 可抑制 CTPE 诱导的恶性转化 BEAS-2B 细胞的迁移、侵袭并增强其凋亡。

IF 2.2 4区 医学 Q3 TOXICOLOGY
Toxicology Research Pub Date : 2024-08-21 eCollection Date: 2024-08-01 DOI:10.1093/toxres/tfae121
Ping Lu, Liu Yang, Yanting Lei, Yuezeng Zhao, Zhihao Tang, Pingping Shang, Xiaolei Zhou, Pengpeng Wang, Wei Wang, Feifei Feng, Qiao Zhang
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引用次数: 0

摘要

长非编码RNA(LncRNA)在细胞恶性转化中发挥着重要作用。本研究旨在探讨 Lnc-ENST00000535078 在煤焦油沥青提取物(CTPE)诱导的永生化人支气管上皮细胞(BEAS-2B)恶性转化中的作用。暴露于 CTPE 的 BEAS-2B 细胞的恶性转化模型。用细胞计数试剂盒-8(CCK-8)检测细胞增殖。细胞集落形成试验用于评估细胞集落。通过 Transwell 分析检测细胞迁移和侵袭。流式细胞术评估细胞周期进展和凋亡状态。通过 RNA 测序筛选差异表达基因。结果显示,Lnc-ENST00000535078在恶性转化的BEAS-2B细胞传至第30代时表达量最高。敲除Lnc-ENST00000535078可抑制恶性转化BEAS-2B细胞的迁移、侵袭和抗凋亡能力。转录组分析发现了 608 个差异基因。CCND1和FOS基因的表达水平与Lnc-ENST00000535078的表达水平呈正相关,因此被筛选出来,这与RNA测序结果一致。总之,Lnc-ENST00000535078的低表达抑制了恶性转化BEAS-2B细胞的迁移和侵袭,并促进了这些细胞的凋亡。Lnc-ENST00000556926可能通过调控CCND1和FOS影响细胞的恶性转化。这项研究可能为干预 CTPE 诱导的肺癌提供了潜在靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Low expression of Lnc-ENST00000535078 inhibits the migration, invasion, and enhances apoptosis of CTPE-induced malignantly transformed BEAS-2B cells.

Long non-coding RNA (LncRNA) plays an important role in malignant transformation of cells. This study aimed to explore the role of Lnc-ENST00000535078 in the malignant transformation of immortalized human bronchial epithelial cells (BEAS-2B) induced by coal tar pitch extract (CTPE). The malignant transformation model of BEAS-2B cells exposed to CTPE. Cell proliferation was examined by Cell counting kit-8 (CCK-8) assay. Colony formation assay was used to assess the colony of cells. Cell migration and invasion were detected by Transwell analysis. Cell cycle progression and apoptotic status were assessed by flow cytometry. Differentially expressed genes were screened by RNA sequencing. The results showed that Lnc-ENST00000535078 expression was highest in malignantly transformed BEAS-2B cells passaged to the 30th generation. Knockdown of Lnc-ENST00000535078 inhibited the migration, invasion and anti-apoptotic abilities of malignantly transformed BEAS-2B cells. Transcriptome analysis found 608 differential genes. CCND1 and FOS genes were screened out because of their levels were positive correlation with the expression of Lnc-ENST00000535078, which were consistent with the RNA sequencing results. In conclusion, Low expression of Lnc-ENST00000535078 inhibits the migration and invasion of malignant transformed BEAS-2B cells and promotes apoptosis in these cells. Lnc-ENST00000556926 might affect the malignant transformation of cells through the regulation of CCND1 and FOS. This study may provide a potential target for intervention in CTPE-induced lung cancer.

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来源期刊
Toxicology Research
Toxicology Research TOXICOLOGY-
CiteScore
3.60
自引率
0.00%
发文量
82
期刊介绍: A multi-disciplinary journal covering the best research in both fundamental and applied aspects of toxicology
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