主要 m6A RNA 修饰调节因子在吸烟冠心病患者中的表达模式和临床价值。

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Epigenetics Pub Date : 2024-12-01 Epub Date: 2024-08-21 DOI:10.1080/15592294.2024.2392400
Huanwei Zhuang, Hua Ouyang, Yangfei Peng, Shuji Gong, Kun Xiang, Le Chen, Jinlan Chen
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引用次数: 0

摘要

尽管 N6-甲基腺苷(m6A)RNA 修饰与人类疾病的关系日益密切,但其在患有冠状动脉疾病(CAD)的吸烟者中的作用机制尚不完全清楚。研究人员从 GEO 中分析了 CAD 患者(吸烟者和非吸烟者)中 30 个 m6A 相关调节因子(MRRE)的表达。构建了支持向量机、随机森林和提名图模型来评估其临床价值。利用共识聚类、主成分分析和ssGSEA构建了患有CAD的吸烟者体内m6A相关调节因子的全貌。缺氧-葡萄糖剥夺(OGD)和 qRT-PCR 被用来验证缺氧对 MRRE 的影响。对患有 CAD 的吸烟者和对照组进行比较后发现,RBM15B、YTHDC2 和 ZC3H13 的表达水平较低。基于三个关键的 MRRE,所有模型都显示出良好的临床价值,并将患有 CAD 的吸烟者分为两个不同的分子亚组。关键 MRRE 与免疫浸润程度之间存在相关性。在 OGD 组中,HUVECs 和 FMC84 小鼠心肌细胞中的三个关键 MRRE 减少。通过缺氧,吸烟可能会降低吸烟者 CAD 中 RBM15B、YTHDC2 和 ZC3H13 的表达水平。我们的研究结果为治疗吸烟者的冠状动脉粥样硬化提供了重要的理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression patterns and clinical value of key m6A RNA modification regulators in smoking patients with coronary artery disease.

Even though N6-methyladenosine (m6A) RNA modifications are increasingly being implicated in human disease, their mechanisms are not fully understood in smokers with coronary artery disease (CAD). Thirty m6A-related regulators' expression (MRRE) in CAD individuals (smokers and non-smokers) were analyzed from GEO. Support Vector Machine, random forest, and nomogram models were constructed to assess its clinical value. Consensus clustering, principal component analysis, and ssGSEA were used to construct a full picture of m6A-related regulators in smokers with CAD. Oxygen-glucose deprivation (OGD) and qRT-PCR were used to validate hypoxia's effect on MRRE. A comparison between smokers with CAD and controls revealed lower expression levels of RBM15B, YTHDC2, and ZC3H13. Based on three key MRREs, all models showed good clinical value, and smokers with CAD were divided into two distinct molecular subgroups. The correlations were found between key MRRE and the degree of immune infiltration. Three key MRREs in HUVECs and FMC84 mouse cardiomyocytes were reduced in the OGD group. Through hypoxia, smoking might reduce the expression levels of RBM15B, YTHDC2, and ZC3H13 in smokers with CAD. Our findings provide an important theoretical basis for the treatment of smokers with CAD.

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来源期刊
Epigenetics
Epigenetics 生物-生化与分子生物学
CiteScore
6.80
自引率
2.70%
发文量
82
审稿时长
3-8 weeks
期刊介绍: Epigenetics publishes peer-reviewed original research and review articles that provide an unprecedented forum where epigenetic mechanisms and their role in diverse biological processes can be revealed, shared, and discussed. Epigenetics research studies heritable changes in gene expression caused by mechanisms others than the modification of the DNA sequence. Epigenetics therefore plays critical roles in a variety of biological systems, diseases, and disciplines. Topics of interest include (but are not limited to): DNA methylation Nucleosome positioning and modification Gene silencing Imprinting Nuclear reprogramming Chromatin remodeling Non-coding RNA Non-histone chromosomal elements Dosage compensation Nuclear organization Epigenetic therapy and diagnostics Nutrition and environmental epigenetics Cancer epigenetics Neuroepigenetics
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