Jens Runge, Sabine Kischkel, Jonas Keiler, Niels Grabow, Klaus-Peter Schmitz, Stefan Siewert, Andreas Wree, Rudolf F Guthoff, Thomas Stahnke
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After orbital exenterations, RGMs were placed in the anterior chamber draining in the subconjunctival space. X-ray contrast was increased by incubation in aqueous iodine solution for subsequent detailed micro-computed tomography (micro-CT)-based visualization and analysis.</p><p><strong>Results: </strong>In contrast to the human and porcine eyes, the stent extended far to the posterior pole with a more pronounced curvature along the globe in the rabbit eyes due to their smaller size. However, dysfunctional deformations were not depicted. Adequate positioning of the stent's inflow area in the anterior chamber and the outflow area in the Tenon space was achieved in both the animal models and the human eye.</p><p><strong>Conclusions: </strong>Micro-CT has proven to be a valuable tool for postoperative <i>ex vivo</i> evaluation of glaucoma drainage devices in its entire complexity. With regard to morphology, the porcine eye is the ideal animal model to test implantation procedures of the RGM. 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X-ray contrast was increased by incubation in aqueous iodine solution for subsequent detailed micro-computed tomography (micro-CT)-based visualization and analysis.</p><p><strong>Results: </strong>In contrast to the human and porcine eyes, the stent extended far to the posterior pole with a more pronounced curvature along the globe in the rabbit eyes due to their smaller size. However, dysfunctional deformations were not depicted. Adequate positioning of the stent's inflow area in the anterior chamber and the outflow area in the Tenon space was achieved in both the animal models and the human eye.</p><p><strong>Conclusions: </strong>Micro-CT has proven to be a valuable tool for postoperative <i>ex vivo</i> evaluation of glaucoma drainage devices in its entire complexity. With regard to morphology, the porcine eye is the ideal animal model to test implantation procedures of the RGM. 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引用次数: 0
摘要
背景:微创青光眼手术(MIGS)已成为治疗原发性开角型青光眼的重要方法。通过异体植入物恢复泪液引流是一种很有前景的治疗方法,其本身也是方法学发展的主题。在目标组织区域的适当定位对于我们自主研发的罗斯托克青光眼微支架(RGM)的性能至关重要。本研究旨在评估两种动物模型和人类供体眼球在 RGM 放置方面的适用性:方法:从兔子、猪和人体捐献者身上获取眼球。方法:从兔子、猪和人体捐献的眼球中获取眼球,眼眶外翻后,将 RGM 置入前房,在结膜下间隙引流。通过在碘水溶液中孵育来增加 X 射线对比度,以便随后进行基于显微计算机断层扫描(micro-CT)的详细观察和分析:结果:与人眼和猪眼相比,兔眼由于体积较小,支架向后极延伸较远,沿眼球的弧度更明显。然而,兔眼并未出现功能障碍变形。在动物模型和人眼中,支架的前房流入区和腱膜间隙流出区都得到了适当的定位:显微计算机断层扫描已被证明是对青光眼引流装置的整个复杂性进行术后活体评估的重要工具。就形态学而言,猪眼是测试 RGM 植入程序的理想动物模型。尽管如此,兔眼的形态也有助于获得成功的植入结果,并为临床前动物研究提供了所有先决条件。
Experimental glaucoma microstent implantation in two animal models and human donor eyes-an ex vivo micro-computed tomography-based evaluation of applicability.
Background: Minimally invasive glaucoma surgery (MIGS) has become an important treatment approach for primary open angle glaucoma. Restoration of aqueous humour drainage by means of alloplastic implants represents a promising treatment option and is itself subject of methodological development. An adequate positioning in the targeted tissue regions is essential is important for the performance of our in-house developed Rostock glaucoma microstent (RGM). The aim of this study was to evaluate the applicability of two animal models and human donor eyes regarding RGM placement.
Methods: Eyes were obtained from rabbits, pigs, and human body donations. After orbital exenterations, RGMs were placed in the anterior chamber draining in the subconjunctival space. X-ray contrast was increased by incubation in aqueous iodine solution for subsequent detailed micro-computed tomography (micro-CT)-based visualization and analysis.
Results: In contrast to the human and porcine eyes, the stent extended far to the posterior pole with a more pronounced curvature along the globe in the rabbit eyes due to their smaller size. However, dysfunctional deformations were not depicted. Adequate positioning of the stent's inflow area in the anterior chamber and the outflow area in the Tenon space was achieved in both the animal models and the human eye.
Conclusions: Micro-CT has proven to be a valuable tool for postoperative ex vivo evaluation of glaucoma drainage devices in its entire complexity. With regard to morphology, the porcine eye is the ideal animal model to test implantation procedures of the RGM. Nevertheless, rabbit eye morphology facilitates successful implantation results and provides all prerequisites for preclinical animal studies.